3.5.1.13: aryl-acylamidase

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For detailed information about aryl-acylamidase, go to the full flat file.

Reaction

Synonyms

AAA, aryl acylamidase, aryl acylaminidase, Azl13, EC 3.5.1.13 AAA-2, EC 3.5.1.13, AAA-1, M-1 amidase, More, PamH, polyamidase, pseudocholinesterase

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.13 aryl-acylamidase

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Results	in table
13	Activating Compound
531	AA Sequence
5	Application
1	CAS Registry Number
6	Cloned(Commentary)
2	Crystallization (Commentary)
3	Disease/ Diagnostics
10	General Information
1	General Stability
3	IC50 Value
206	Inhibitors
3	kcat/KM [mM/s]
15	Ki Value [mM]
51	KM Value [mM]
13	Localization
8	Metals/Ions
14	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
92	Organism
2	PDB ID
40	pH Optimum
9	pH Range
7	pH Stability
2	pI Value
1	Posttranslational Modification
13	Protein Variants
23	Purification (Commentary)
3	Reaction
3	Reaction Type
90	Reference
54	Source Tissue
50	Specific Activity [micromol/min/mg]
7	Storage Stability
205	Substrates and Products (Substrate)
14	Subunits
40	Synonyms
1	Systematic Name
33	Temperature Optimum [°C]
3	Temperature Range [°C]
25	Temperature Stability [°C]
19	Turnover Number [1/s]

Cloned

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Cloned on EC 3.5.1.13 - aryl-acylamidase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Homo sapiens

-

697867

cloned in a modified pET21 vector encoding a 6x-histidine tag at the carboxyl terminus and overexpressed in Escherichia coli BL21 (DE3)

bacterium CSBL00001

C3UWD1

752657

DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic library construction and cloning in Escherichia coli strains NM522 and DH5alpha, expression in Escherichia coli strain BL21(DE3)

bacterium CSBL00001

C3UWD1

713035

gene azl13, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Streptomyces sp.

A0A088BHP3

733190

gene pamH, DNA and amino acid sequence determination and analysis, sequence comparison, cloning and expression of His6-tagged PamH in Escherichia coli strains DH5alpha and BL21 (DE3), respectively

Paracoccus sp. M1-1

F6N111

718672

gene pamh, genetic organization, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3). The enzyme is only successfully expressed using autoinduction media under low-temperature incubation at 25°C, otherwise, the expressed proteins forms inclusion bodies

Paracoccus sp. M1-1

F6N111

718672