3.4.24.63: meprin B
This is an abbreviated version!
For detailed information about meprin B, go to the full flat file.
Word Map on EC 3.4.24.63
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3.4.24.63
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alzheimer
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amyloid
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abeta
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gamma-secretase
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beta-amyloid
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plaque
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amyloid-beta
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beta-site
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amyloidogenic
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alpha-secretase
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aspartyl
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cerebral
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presenilins
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senile
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neurodegenerative
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bace-1
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swedish
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sappalpha
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neuropathology
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tau
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neurotoxic
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app-cleaving
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neuroblastoma
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beta-peptide
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ectodomains
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dementia
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protein-cleaving
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medicine
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nicastrin
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drug development
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amyloidogenesis
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tangles
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isostere
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memapsin
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peptidomimetic
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abeta1-40
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appswe
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ad-like
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hydroxyethylene
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neurofibrillary
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adam10
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beta-protein
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ad-associated
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endoproteolytic
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insulin-degrading
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non-amyloidogenic
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betaapp
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neprilysin
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pharmacology
- 3.4.24.63
- alzheimer
-
amyloid
- abeta
- gamma-secretase
- beta-amyloid
- plaque
- amyloid-beta
-
beta-site
-
amyloidogenic
- alpha-secretase
-
aspartyl
- cerebral
-
presenilins
-
senile
- neurodegenerative
- bace-1
-
swedish
-
sappalpha
-
neuropathology
- tau
-
neurotoxic
-
app-cleaving
- neuroblastoma
- beta-peptide
- ectodomains
- dementia
-
protein-cleaving
- medicine
-
nicastrin
- drug development
-
amyloidogenesis
-
tangles
-
isostere
-
memapsin
-
peptidomimetic
- abeta1-40
-
appswe
-
ad-like
-
hydroxyethylene
-
neurofibrillary
- adam10
- beta-protein
-
ad-associated
-
endoproteolytic
-
insulin-degrading
-
non-amyloidogenic
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betaapp
- neprilysin
- pharmacology
Reaction
Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His5-/-Leu-, -Leu6-/-Cys-, -Ala14-/-Leu- and -Cys19-/-Gly- bonds in insulin B chain =
Synonyms
beta-secretase, cell surface sheddase, h-meprin beta, MEP1B, mephrin beta, meprin A subunit beta, Meprin b, meprin B metalloprotease, meprin beta, meprin beta metalloproteinase, meprin metalloprotease, meprin metalloproteinase, meprin metalloproteinase beta, meprin-beta, meprinbeta, metalloprotease meprin, metalloprotease meprin B, metalloprotease meprin beta, metalloproteinase meprin beta, Mmepb, More, mouse meprin beta, procollagen proteinase, Rmepb
ECTree
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Engineering
Engineering on EC 3.4.24.63 - meprin B
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D240A
site-directed mutagenesis, the D204A mutant shows almost no signal at the cell surface, but strongly colocalized with the endoplasmic reticulum marker, low cell surface activity of D204A enzyme
D47A
the mutation is localized in the pro-peptide of the protease, the mutant is transported to the cell surface and exhibit expression comparable with wild-type meprin beta
G32R
naturally occuring mutant, the mutation is localized in the pro-peptide of the protease and identified in endometrium cancer, the mutant is transported to the cell surface and exhibit expression comparable with wild-type meprin beta, the mutant is more active against a peptide substrate (meprin beta-specific peptide substrate that is linked to a fluorogenic group (MCA) at the N-terminus and a quencher (DPN) at the C-terminus) and the interleukin-6 receptor than wild-type meprin beta. The change to an arginine residue at position 32 represents an additional activation site used by furin-like proteases in the Golgi, which consequently leads to reduced shedding by ADAM17. The meprin beta G32R variant assesses cell proliferation, invasion through a collagen IV matrix, and outgrowth from tumor spheroids. Increased meprin beta G32R activity at the cell surface reduces cell proliferation, but increases cell invasion. The G32R meprin beta variant shows increased activity. Phenotype, overview
G32R/R61S
site-directed mutagenesis, the double mutant has two altered sites that can no longer be proteolytically cleaved and cannot be activated or shedded
additional information
the expression patterns and cellular localizations of the two amino acid exchange variants do not differ from that of wild-type meprin beta
additional information
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homozygous knockout mice deficient in beta-subunit are produced from wild-type C57BL/6 mice with 129/Sv F2 embryonic stem cells and subjected to ischemia-reperfusion, they show a higher susceptability to renal injury, while wild-type mice show rapid redistribution of meprin beta-subunits in proximal tubule cells, overview
additional information
generation and genotyping of meprin beta-knockout mice
additional information
generation of knock-out mice deficient for meprin alpha, phenotype, overview
additional information
generation of meprin B deficient Mep1beta-/- mice, penotype overview. Maturation of type I procollagen is reduced in skin and primary fibroblasts from Mep1b?/? mice, the skin shows reduced tensile strength
additional information
generation of bleomycin-treated meprin alpha, meprin beta, and the double meprins alphabeta knock-out (KO) mice. No difference in lung function parameters and no change in inflammatory cells infiltrating the lung are observed between wild-type and all meprins KO mice after 14 days bleomycin. Morphological analysis in the bleomycin-treated mice reveals a decrease in collagen deposition and tissue density in meprin beta KO mice, but not in meprin alpha and meprin alphabeta KO mice. This finding is accompanied by localization of meprin beta to epithelial cells in regions with immature collagen in mice
additional information
generation of enzyme knockout Mep1beta-/- mice from wild-type C57/BL6N, isolation of bone marrow-derived macrophages and phenotype, overview
additional information
generation of meprin alphabeta knockout mice (alphabetaKO) which lack endogenous meprins, phenotype after CoCl2 exposure, overview
additional information
generation of meprin beta knockout mice. Knockout of meprin beta leads to increased sAPPalpha secretion in cortical neurons. Decrease of Abeta2-40 and increase of mature APP in primary neurons of meprin beta knockout mice
additional information
meprin beta silencing by nanoparticle-based application of meprin beta specific siRNA
additional information
Mus musculus C57/BL6N
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generation of enzyme knockout Mep1beta-/- mice from wild-type C57/BL6N, isolation of bone marrow-derived macrophages and phenotype, overview
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additional information
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generation of meprin alphabeta knockout mice (alphabetaKO) which lack endogenous meprins, phenotype after CoCl2 exposure, overview
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additional information
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generation of meprin beta knockout mice. Knockout of meprin beta leads to increased sAPPalpha secretion in cortical neurons. Decrease of Abeta2-40 and increase of mature APP in primary neurons of meprin beta knockout mice
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additional information
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generation and genotyping of meprin beta-knockout mice
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