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3.4.24.63: meprin B

This is an abbreviated version!
For detailed information about meprin B, go to the full flat file.

Word Map on EC 3.4.24.63

Reaction

Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His5-/-Leu-, -Leu6-/-Cys-, -Ala14-/-Leu- and -Cys19-/-Gly- bonds in insulin B chain =

Synonyms

beta-secretase, cell surface sheddase, h-meprin beta, MEP1B, mephrin beta, meprin A subunit beta, Meprin b, meprin B metalloprotease, meprin beta, meprin beta metalloproteinase, meprin metalloprotease, meprin metalloproteinase, meprin metalloproteinase beta, meprin-beta, meprinbeta, metalloprotease meprin, metalloprotease meprin B, metalloprotease meprin beta, metalloproteinase meprin beta, Mmepb, More, mouse meprin beta, procollagen proteinase, Rmepb

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.63 meprin B

Purification

Purification on EC 3.4.24.63 - meprin B

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
C3H/He mouse
-
expressed in human kidney cell line 293, papain-solubilized
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isolation of meprins from the mouse kidneys by immunoaffinity chromatography using mannan-binding protein resin
papain-solubilized
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purified from stably transfected human embryonic kidney HEK-293 cells
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recombinant Flag-tagged meprin beta from HEK-293 cells by protein G affinity chromatography. Co-immunoprecipitation of C-terminal Flag-tagged meprin beta and C-terminal Myc-tagged ADAM proteases is performed in HEK-293T cells
recombinant His-tagged enzyme
-
recombinant His-tagged enzyme from Spodoptera frugiperda Sf9 insect cells by affinity chromatography
recombinant His6-tagged enzyme from Pichia pastoris strain X33 or SMD1168H by nickel affinity chromatography, enzyme activation by trypsin, and hydrophobic interaction chromatography, followed by gel filtration
recombinant His6-tagged meprin beta protein (1-648) from human HEK-293 cells by metal-chelating affinity chromatography
recombinant meprin B from HEK-293 cells
recombinant N-terminally His-tagged human meprin beta zymogen from Hi5 insect cells by nickel affinity chromatography
recombinant N-terminally His-tagged meprin beta from HEK-293 cells by nickel affinity chromatography
recombinant N-terminally His6-tagged meprin beta from Pichia pastoris strain X33 by nickel affinity chromatography, activation of the enzyme by trypsin, followed by hydrophobic interaction chromatography, and gel filtration
recombinant soluble tail switch mutant pro-meprinbeta from BT1-TN-5B1-4 insect cells by nickel affinity chromatography after activation to meprinbeta by trypsin, trypsin is removed by chicken ovomucoid affinity chromatography
-