Cloned (Comment) | Organism |
---|---|
recombinant expression of FLAG-tagged enzyme mutants in HEK-293 and COS-7 cells, recombinant expression of N-terminally His-tagged human meprin beta zymogen in Hi5 insect cells via viral transfection method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D240A | site-directed mutagenesis, the D204A mutant shows almost no signal at the cell surface, but strongly colocalized with the endoplasmic reticulum marker, low cell surface activity of D204A enzyme | Homo sapiens |
D245A | site-directed mutagenesis | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | calcium negatively regulates meprin beta activity and attenuates substrate cleavage | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | physiologic calcium concentrations display a dual role by (1) directly inhibiting meprin beta activity and (2) supporting meprin beta folding during biosynthesis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid precursor protein + H2O | Homo sapiens | - |
? | - |
? | |
dentin phosphoprotein + H2O | Homo sapiens | - |
? | - |
? | |
dentin sialophosphoprotein + H2O | Homo sapiens | - |
? | - |
? | |
mucin-2 + H2O | Homo sapiens | - |
? | - |
? | |
Procollagen + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16820 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | deglycosylation of recombinant enzyme by peptide-N-glycosidase | Homo sapiens |
proteolytic modification | the recombinant N-terminally His-tagged human meprin beta zymogen is activated by cleavage with trypsin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged human meprin beta zymogen from Hi5 insect cells by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(7-methyloxycoumarin-4-yl)acetyl-EDEDED-NH2-(2,4-dinitrophenyl) + H2O | - |
Homo sapiens | ? | - |
? | |
amyloid precursor protein + H2O | - |
Homo sapiens | ? | - |
? | |
amyloid precursor protein + H2O | recombinant soluble human APP695, cleavage at the beta-secretase site | Homo sapiens | ? | - |
? | |
dentin phosphoprotein + H2O | - |
Homo sapiens | ? | - |
? | |
dentin sialophosphoprotein + H2O | - |
Homo sapiens | ? | - |
? | |
mucin-2 + H2O | - |
Homo sapiens | ? | - |
? | |
Procollagen + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
meprin beta | - |
Homo sapiens |
meprin beta metalloproteinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | calcium negatively regulates meprin beta activity and attenuates substrate cleavage | Homo sapiens |
physiological function | meprin beta metalloproteinase is an important enzyme in extracellular matrix turnover, inflammation, and neurodegeneration in humans | Homo sapiens |