Information on EC 3.4.24.63 - meprin B

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The expected taxonomic range for this enzyme is: Euteleostomi

EC NUMBER
COMMENTARY hide
3.4.24.63
-
RECOMMENDED NAME
GeneOntology No.
meprin B
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of proteins, including azocasein, and peptides. Hydrolysis of -His5-/-Leu-, -Leu6-/-Cys-, -Ala14-/-Leu- and -Cys19-/-Gly- bonds in insulin B chain
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
150679-52-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Mus musculus BALB/c
-
SwissProt
Manually annotated by BRENDA team
strain C57BL
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6N
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(7-methoxycoumarin-4-yl)acetyl-YVADAPK-(K-epsilon-DNP) + H2O
?
show the reaction diagram
-
-
-
-
?
(7-methyloxycoumarin-4-yl) acetyl-EDEDED-2,4-dinitrophenyl + H2O
?
show the reaction diagram
-
-
-
-
?
2-aminobenzoyl-MGWM--DEIDK(2,4-dinitrophenyl)SG-OH + H2O
?
show the reaction diagram
-
-
-
?
Abz-VKMDAE-EDDnp + H2O
?
show the reaction diagram
-
wild-type beta-site fluorogenic substrate sequence
-
-
?
Abz-VNLDAE-EDDnp + H2O
?
show the reaction diagram
-
Swedish mutant beta-site fluorogenic substrate sequence
-
-
?
actin + H2O
?
show the reaction diagram
alpha-MSH + H2O
?
show the reaction diagram
alpha-secretase + H2O
?
show the reaction diagram
meprin beta-mediated activation of the alpha-secretase
-
-
?
amyloid precursor protein + H2O
?
show the reaction diagram
amyloid precursor protein + H2O
amyloid beta peptides
show the reaction diagram
meprin beta initially sheds amyloid precursor protein, releasing different amyloid beta species with several cleavage sites identical with or proximal to the known beta-secretase cleavage site
-
-
?
amyloid precursor protein + H2O
amyloid precursor protein N-terminal fragments + amyloid beta protein
show the reaction diagram
amyloid precursor protein APP751 + H2O
amyloid precursor protein N-terminal fragments + amyloid beta protein
show the reaction diagram
-
recombinant human substrate, coexpressed with the enzyme in HEK-293T cells, or exogenous soluble meprin beta incubated with APP751 stably overexpressing 7WD10 cells, is able to produce amyloid precursor fragment N-APP20
-
-
?
antileucoproteinase + H2O
?
show the reaction diagram
a serine protease inhibitor
-
-
?
azocasein + H2O
?
show the reaction diagram
Boc-CCK8NH2 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Bradykinin + H2O
?
show the reaction diagram
-
-
-
?
CCK4 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
CCK5 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
CCK6 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
CCK7 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
CCK8 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
CCK8NH2 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
cerulein + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Cholecystokinin + H2O
?
show the reaction diagram
-
-
-
?
cholecystokinin 8 sulfate + H2O
?
show the reaction diagram
-
-
-
?
collagen I + H2O
?
show the reaction diagram
-
-
-
?
Collagen IV + H2O
?
show the reaction diagram
-
-
-
?
dentin phosphoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
dentin sialophosphoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
E-cadherin + H2O
?
show the reaction diagram
elafin + H2O
?
show the reaction diagram
a serine protease inhibitor
-
-
?
fibrillar procollagen type I + H2O
fibrillar collagen type I + fibrillar collagen type I propeptide
show the reaction diagram
fibrillar procollagen type III + H2O
fibrillar collagen type III + fibrillar collagen type I propeptide
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
gastrin 17 + H2O
?
show the reaction diagram
Gelatin + H2O
?
show the reaction diagram
-
-
-
?
Glucagon + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
GRP-(14-27) + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Insulin B-chain + H2O
?
show the reaction diagram
interleukin-1beta precursor + H2O
interleukin-1beta + interleukin-1beta propeptide
show the reaction diagram
proteolytic cleavage to a biologically active form
-
-
?
interleukin-6 + H2O
?
show the reaction diagram
Laminin + H2O
?
show the reaction diagram
-
-
-
?
lymphoepithelial Kazal-type-related inhibitor + H2O
?
show the reaction diagram
a serine protease inhibitor
-
-
?
miniprocollagen alpha1(I) homotrimers + H2O
?
show the reaction diagram
-
-
-
-
?
MMP1 protein + H2O
?
show the reaction diagram
Muc2 protein + H2O
?
show the reaction diagram
mucin-2 + H2O
?
show the reaction diagram
-
-
-
-
?
N-Benzoyl-Tyr 4-aminobenzoate + H2O
?
show the reaction diagram
neuropeptide Y + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
nidogen 1 + H2O
?
show the reaction diagram
nidogen-1 + H2O
?
show the reaction diagram
orcokinin + H2O
?
show the reaction diagram
osteopontin + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Procollagen + H2O
?
show the reaction diagram
-
-
-
-
?
procollagen I + H2O
collagen I + propeptide of collagen III
show the reaction diagram
protein kinase A + H2O
?
show the reaction diagram
the enzyme cleaves at defined sites, isoform-specific interactions between the catalytic subunit of PKA (PKA C) and meprins, overview
-
-
?
protein kinase A catalytic subunit Calpha + H2O
?
show the reaction diagram
the enzyme cleaves at defined sites, cytosolic-enriched kidney proteins from meprin alphabeta double knockout mice, and purified forms of recombinant mouse PKA Calpha, Cbeta1, and Cbeta2, are incubated with activated forms of either homomeric meprinA, EC 3.4.24.18, or meprin B, product analysis by mass spectrometry, overview. Meprin B cleaves all three PKA C isoforms
-
-
?
protein kinase A catalytic subunit Cbeta1 + H2O
?
show the reaction diagram
the enzyme cleaves at defined sites, cytosolic-enriched kidney proteins from meprin alphabeta double knockout mice, and purified forms of recombinant mouse PKA Calpha, Cbeta1, and Cbeta2, are incubated with activated forms of either homomeric meprinA, EC 3.4.24.18, or meprin B, product overview. Meprin B cleaves all three PKA C isoforms
-
-
?
protein kinase A catalytic subunit Cbeta2 + H2O
?
show the reaction diagram
the enzyme cleaves at defined sites, cytosolic-enriched kidney proteins from meprin alphabeta double knockout mice, and purified forms of recombinant mouse PKA Calpha, Cbeta1, and Cbeta2, are incubated with activated forms of either homomeric meprinA, EC 3.4.24.18, or meprin B, product overview. Meprin B cleaves all three PKA C isoforms
-
-
?
sCCK8NH2 + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Secretin + H2O
?
show the reaction diagram
-
peptide of the gastrointestinal tract
-
?
Succinyl-Ala-Ala-Ala 4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
-
Succinyl-Ala-Ala-Val-Ala 4-nitroanilide + H2O
?
show the reaction diagram
-
-
-
-
-
tenascin-C + H2O
?
show the reaction diagram
tenascin-C + H2O
tenascin-C peptide fragments
show the reaction diagram
-
mapping of proteolytic fragments generated by meprinbeta from the chicken tenascin-C and the human recombinant 250 kDa TN-C variant. In chicken tenascin-C, meprinbeta processes all three major splicing variants by removal of 10 kDa N-terminal and 38 kDa C-terminal peptides, leaving a large central part of subunits intact. A similar cleavage pattern exists for large human tenascin-C variant where two N-terminal peptides of 10 and 15 kDa and two C-terminal fragments of 40 and 55 kDa are removed from the intact subunit. N-terminal sequencing reveals the exact amino acid positions of cleavage sites. In both chicken and human tenascin-C N-terminal cleavages occur just before and/or after the heptad repeats involved in subunit oligomerization. In the human protein, an additional cleavage site is identified in the alternative fibronectin type III repeat, and a unique cleavage by meprinbeta is located to the 7th constant fibronectin type III repeat in both chicken and human tenascin-C, cleavage at this site removes the C-terminal domain involved in its anti-adhesive activity
-
-
?
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly + H2O
?
show the reaction diagram
-
synthetic octapeptide, derived from insulin B-chain
-
-
-
villin + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-secretase + H2O
?
show the reaction diagram
Q16820
meprin beta-mediated activation of the alpha-secretase
-
-
?
amyloid precursor protein + H2O
?
show the reaction diagram
amyloid precursor protein + H2O
amyloid beta peptides
show the reaction diagram
Q16820
meprin beta initially sheds amyloid precursor protein, releasing different amyloid beta species with several cleavage sites identical with or proximal to the known beta-secretase cleavage site
-
-
?
amyloid precursor protein + H2O
amyloid precursor protein N-terminal fragments + amyloid beta protein
show the reaction diagram
-
meprin beta can also process amyloid precursor protein in a manner reminiscent of beta-secretase, identification of cleavage sites of meprin beta in the amyloid beta sequence of the wild-type and Swedish mutant of amyloid precursor protein at positions p1 and p2, thereby generating amyloid beta variants starting at the first or second amino acid residue, mass spectrometry analysis, overview
-
-
?
dentin phosphoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
dentin sialophosphoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
E-cadherin + H2O
?
show the reaction diagram
fibrillar procollagen type I + H2O
fibrillar collagen type I + fibrillar collagen type I propeptide
show the reaction diagram
fibrillar procollagen type III + H2O
fibrillar collagen type III + fibrillar collagen type I propeptide
show the reaction diagram
Fibronectin + H2O
?
show the reaction diagram
MMP1 protein + H2O
?
show the reaction diagram
Muc2 protein + H2O
?
show the reaction diagram
mucin-2 + H2O
?
show the reaction diagram
-
-
-
-
?
nidogen 1 + H2O
?
show the reaction diagram
nidogen-1 + H2O
?
show the reaction diagram
Procollagen + H2O
?
show the reaction diagram
-
-
-
-
?
procollagen I + H2O
collagen I + propeptide of collagen III
show the reaction diagram
-
meprin beta removes both the C- and N-propeptides of type I procollagen, subsequently releasing fibril-forming mature collagen molecules. The C-terminal cleavage sites in the proalpha1(I) chain generated by the enzyme is identified as Ala1218/Asp1219, identical to the BMP-1 cleavage site, and also Arg1227/Asp1228, nine residues C-terminal to the BMP-1 cleavage site
-
-
?
protein kinase A + H2O
?
show the reaction diagram
Q61847
the enzyme cleaves at defined sites, isoform-specific interactions between the catalytic subunit of PKA (PKA C) and meprins, overview
-
-
?
tenascin-C + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
physiologic calcium concentrations display a dual role by (1) directly inhibiting meprin beta activity and (2) supporting meprin beta folding during biosynthesis
additional information
-
metalloendopeptidase according to inhibition profile
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
strong
2-mercaptoethanol
-
-
3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid
-
competitive inhibition, binding occurs in meprin beta active site
actinonin
batimastat
-
Ca2+
-
calcium negatively regulates meprin beta activity and attenuates substrate cleavage
captopril
-
cysteine
-
-
DL-Pro-DL-Leu-Gly-NH2
-
EDTA
-
strong
fetuin-A
-
-
N-[1-[(1-amino-1-oxopropan-2-yl)amino]-3-(naphthalen-1-yl)-1-oxopropan-2-yl]-4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enamide
-
N-[4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enoyl]-3-methylvalyl-N-(2-aminoethyl)-DL-alaninamide
-
N4-hydroxy-N1-[3-(1H-indol-3-yl)-1-(methylamino)-1-oxopropan-2-yl]-2-(2-methylpropyl)butanediamide
-
NF449
-
Nf449 is a suramin analogue, mixed competitive/noncompetitive inhibition, binding occurs in meprin beta active site
Pro-Leu-Gly-hydroxamate
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Trypsin
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000007
(7-methoxycoumarin-4-yl)acetyl-YVADAPK-(K-epsilon-DNP)
-
pH 7.5, 25C
-
0.032
Abz-VKMDAE-EDDnp
-
pH 7.5, 37C
-
0.015
Abz-VNLDAE-EDDnp
-
pH 7.5, 37C
-
0.00073
actin
-
pH 7.5, 37C
0.282
alpha-MSH
-
pH 7.5, 37C, mutant K185Y
0.00104
bradykinin
-
pH 7.5, 37C
0.211 - 0.22
cholecystokinin 8 sulfate
0.0071
gastrin
-
pH 7.5, 37C
0.00104 - 0.0296
gastrin 17
0.22
glucagon
-
pH 7.5, 37C
0.0481
GRP-(14-27)
-
pH 7.5, 37C
0.1
orcokinin
-
pH 7.5, 37C
-
0.211
sCCK8NH2
-
pH 7.5, 37C
-
0.00117
villin
-
pH 7.5, 37C
-
additional information
additional information
-
Michaelis-Menten enzyme kinetics, overview. Meprin B-mediated cleavage of PKA C? occurs at a rate consistent with that of other physiologically relevant meprin substrates
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.5
Abz-VKMDAE-EDDnp
Homo sapiens
-
pH 7.5, 37C
-
20.9
Abz-VNLDAE-EDDnp
Homo sapiens
-
pH 7.5, 37C
-
0.625
actin
Rattus norvegicus
-
pH 7.5, 37C
15
alpha-MSH
Mus musculus
-
pH 7.5, 37C, mutant K185Y
11
bradykinin
Rattus norvegicus
-
pH 7.5, 37C
13.9 - 15
cholecystokinin 8 sulfate
12.4
gastrin
Mus musculus
-
pH 7.5, 37C
10.5 - 17.8
gastrin 17
15.9 - 26.8
glucagon
12.6
GRP-(14-27)
Mus musculus
-
pH 7.5, 37C
23.4
orcokinin
Mus musculus
-
pH 7.5, 37C
-
13.9
sCCK8NH2
Mus musculus
-
pH 7.5, 37C
-
150
villin
Rattus norvegicus
-
pH 7.5, 37C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
480
Abz-VKMDAE-EDDnp
Homo sapiens
-
pH 7.5, 37C
206916
1410
Abz-VNLDAE-EDDnp
Homo sapiens
-
pH 7.5, 37C
206917
860
actin
Rattus norvegicus
-
pH 7.5, 37C
796
128200
villin
Rattus norvegicus
-
pH 7.5, 37C
41957
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000008
3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid
-
pH 7.5, 25C
0.002
actinonin
0.018
batimastat
pH and temperature not specified in the publication
0.41
captopril
pH and temperature not specified in the publication
0.014
DL-Pro-DL-Leu-Gly-NH2
pH and temperature not specified in the publication
0.0089
galardin
pH and temperature not specified in the publication
0.0074
N4-hydroxy-N1-[3-(1H-indol-3-yl)-1-(methylamino)-1-oxopropan-2-yl]-2-(2-methylpropyl)butanediamide
pH and temperature not specified in the publication
0.00007
NF449
-
pH 7.5, 25C
additional information
additional information
-
inhibition kinetics, overview
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00008
3-[(E)-[4-formyl-5-hydroxy-6-methyl-3-[(phosphonooxy)methyl]pyridin-2-yl]diazenyl]-7-nitronaphthalene-1,5-disulfonic acid
Homo sapiens
-
pH 7.5, 25C
0.002
actinonin
Homo sapiens
-
pH and temperature not specified in the publication
0.4
N-[1-[(1-amino-1-oxopropan-2-yl)amino]-3-(naphthalen-1-yl)-1-oxopropan-2-yl]-4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enamide
Homo sapiens
Q16820
pH and temperature not specified in the publication
0.2
N-[4-(hydroxyamino)-2-(2-methylpropyl)pent-4-enoyl]-3-methylvalyl-N-(2-aminoethyl)-DL-alaninamide
Homo sapiens
Q16820
pH and temperature not specified in the publication
0.000022
NF449
Homo sapiens
-
pH 7.5, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.367 - 0.467
-
Tyr-Leu-Val-Cys(SO3-)-Gly-Glu-Arg-Gly as substrate
1.82
-
insulin B-chain as substrate
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 10
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
whereas the expression of meprinbeta and tenascin-C does not overlap in normal colon tissue, inflamed lesions of the mucosa from patients with Crohn's disease exhibit many meprinbeta-positive leukocytes in regions where tenascin-C is strongly induced
Manually annotated by BRENDA team
-
recombinant enzyme
Manually annotated by BRENDA team
terminally differentiated
Manually annotated by BRENDA team
-
meprin beta is expressed in leukocytes of the draining lymph node of the intestine, regardless of the inflammatory status of the animal
Manually annotated by BRENDA team
-
leukocytes and cortical and medullary macrophages, the enzyme is not decreased during intestinal inflammation
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
meprin beta is a secreted homodimeric multidomain type-I membrane metallopeptidase
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
76100
-
homooligomeric isoform, active, monomer, MALDI-TOF mass spectroscopy
85500
-
homooligomeric isoform, latent, monomer, MALDI-TOF mass spectroscopy
145000
-
-
154000
-
homooligomeric isoform, active, dimer, MALDI-TOF mass spectroscopy
171000
-
homooligomeric isoform, latent, dimer, MALDI-TOF mass spectroscopy
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 73000, SDS-PAGE; x * 73000, SDS-PAGE; x * 73000, SDS-PAGE; x * 73000, SDS-PAGE
homodimer
oligomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure analysis of the ectodomain of dimeric human meprin beta, PDB codes 4GWN and 4GWM
zymogen promeprin beta and the major fragment of the meprin beta-ectoprotein, X-ray diffraction structure determination and analysis, modeling
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58
-
t1/2: 2.5 min, less stable than meprin A
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
C3H/He mouse; papain-solubilized
-
expressed in human kidney cell line 293, papain-solubilized
-
isolation of meprins from the mouse kidneys by immunoaffinity chromatography using mannan-binding protein resin
-
papain-solubilized
-
purified from stably transfected human embryonic kidney HEK-293 cells
-
recombinant His-tagged enzyme
-
recombinant His-tagged enzyme from Spodoptera frugiperda Sf9 insect cells by affinity chromatography
-
recombinant His6-tagged meprin beta protein (1-648) from human HEK-293 cells by metal-chelating affinity chromatography
recombinant N-terminally His-tagged human meprin beta zymogen from Hi5 insect cells by nickel affinity chromatography
-
recombinant soluble tail switch mutant pro-meprinbeta from BT1-TN-5B1-4 insect cells by nickel affinity chromatography after activation to meprinbeta by trypsin, trypsin is removed by chicken ovomucoid affinity chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
4 genes encoding alpha and beta subunits of the enzyme are located on chromosome 20, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, sequence comparisons, overview; 4 genes encoding alpha and beta subunits of the enzyme are located on chromosome 20, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, sequence comparisons, overview; 4 genes encoding alpha and beta subunits of the enzyme are located on chromosome 20, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, sequence comparisons, overview; 4 genes encoding alpha and beta subunits of the enzyme are located on chromosome 20, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, sequence comparisons, overview
analysis of expression level of meprinbeta in high IgA mouse (time dependent)
-
expression of mepbetaDELTATM, meprin beta coding region lacking the transmembrane and intracellular domains with V5 and hexahistidine C-terminal tags (termed as mepbetaDELTATM) in HEK-293F cells
-
expression of the soluble tail switch mutant pro-meprinbeta in BT1-TN-5B1-4 insect cells using the baculovirus transfection system
-
homooligomeric meprin B produced by transfecting cells with alpha or beta cDNA
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mouse, Mep-1beta structural gene, localized to chromosome 18
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rat, expressed in human kidney cell line 293, the expressed protein displays no detectable peptidase activity unless it is activated by removal of amino-terminal prosequence by limited trypsin digestion
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recombinant expression of C-terminally His6-tagged meprin beta protein (1-648) in human HEK-293 cells
recombinant expression of FLAG-tagged enzyme mutants in HEK-293 and COS-7 cells, recombinant expression of N-terminally His-tagged human meprin beta zymogen in Hi5 insect cells via viral transfection method
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recombinant expression of His-tagged enzyme in Spodoptera frugiperda Sf9 insect cells
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recombinant His-tagged enzyme, cDNA transfected into human embryonic kidney 293 cells
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recombinantly expressed
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transient co-expression of amyloid precursor protein APP751 and meprin beta in HEK293T cells. Generation of BACE1/2-KO mouse fibroblasts overexpressing human APP695 isoform and human meprin beta
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in the ileal mucosa of Crohn's disease patients, mRNA levels of meprin beta are decreased
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D240A
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site-directed mutagenesis, the D204A mutant shows almost no signal at the cell surface, but strongly colocalized with the endoplasmic reticulum marker, low cell surface activity of D204A enzyme
D245A
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site-directed mutagenesis
E90A
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soluble inactive mutant of meprin beta
K185Y
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site-directed mutagenesis
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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results suggest that meprinbeta may play a protective role against the progression of renal injury through the degradation of extracellular matrix and bioactive peptides
pharmacology