Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.24.63 extracted from

  • Bien, J.; Jefferson, T.; Causevic, M.; Jumpertz, T.; Munter, L.; Multhaup, G.; Weggen, S.; Becker-Pauly, C.; Pietrzik, C.
    The metalloprotease meprin beta generates amino terminal-truncated amyloid beta peptide species (2012), J. Biol. Chem., 287, 33304-33313.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
transient co-expression of amyloid precursor protein APP751 and meprin beta in HEK293T cells. Generation of BACE1/2-KO mouse fibroblasts overexpressing human APP695 isoform and human meprin beta Homo sapiens

Protein Variants

Protein Variants Comment Organism
E90A soluble inactive mutant of meprin beta Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
actinonin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
Abz-VNLDAE-EDDnp pH 7.5, 37°C Homo sapiens
0.032
-
Abz-VKMDAE-EDDnp pH 7.5, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane meprin beta is a type I transmembrane protein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ metalloprotease Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amyloid precursor protein + H2O Homo sapiens meprin beta can also process amyloid precursor protein in a manner reminiscent of beta-secretase, identification of cleavage sites of meprin beta in the amyloid beta sequence of the wild-type and Swedish mutant of amyloid precursor protein at positions p1 and p2, thereby generating amyloid beta variants starting at the first or second amino acid residue, mass spectrometry analysis, overview amyloid precursor protein N-terminal fragments + amyloid beta protein
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q16820
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain meprin beta mRNA levels are increased in brains of Alzheimer's disease patients compared with age-matched control individuals Homo sapiens
-
additional information wide expression pattern Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-VKMDAE-EDDnp + H2O wild-type beta-site fluorogenic substrate sequence Homo sapiens ?
-
?
Abz-VNLDAE-EDDnp + H2O Swedish mutant beta-site fluorogenic substrate sequence Homo sapiens ?
-
?
amyloid precursor protein + H2O meprin beta can also process amyloid precursor protein in a manner reminiscent of beta-secretase, identification of cleavage sites of meprin beta in the amyloid beta sequence of the wild-type and Swedish mutant of amyloid precursor protein at positions p1 and p2, thereby generating amyloid beta variants starting at the first or second amino acid residue, mass spectrometry analysis, overview Homo sapiens amyloid precursor protein N-terminal fragments + amyloid beta protein
-
?
amyloid precursor protein + H2O catalytic properties and cleavage sites of meprin beta within different amyloid precursor protein peptides, overview Homo sapiens amyloid precursor protein N-terminal fragments + amyloid beta protein
-
?
amyloid precursor protein APP751 + H2O recombinant human substrate, coexpressed with the enzyme in HEK-293T cells, or exogenous soluble meprin beta incubated with APP751 stably overexpressing 7WD10 cells, is able to produce amyloid precursor fragment N-APP20 Homo sapiens amyloid precursor protein N-terminal fragments + amyloid beta protein
-
?

Synonyms

Synonyms Comment Organism
meprin beta
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.5
-
Abz-VKMDAE-EDDnp pH 7.5, 37°C Homo sapiens
20.9
-
Abz-VNLDAE-EDDnp pH 7.5, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the astacin protease family Homo sapiens
physiological function processing of the amyloid precursor protein is of critical importance, the enzyme cleaves amyloid precursor protein and liberates soluble N-terminal amyloid precursor protein fragments Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
480
-
Abz-VKMDAE-EDDnp pH 7.5, 37°C Homo sapiens
1410
-
Abz-VNLDAE-EDDnp pH 7.5, 37°C Homo sapiens