Cloned (Comment) | Organism |
---|---|
transient co-expression of amyloid precursor protein APP751 and meprin beta in HEK293T cells. Generation of BACE1/2-KO mouse fibroblasts overexpressing human APP695 isoform and human meprin beta | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E90A | soluble inactive mutant of meprin beta | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actinonin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
Abz-VNLDAE-EDDnp | pH 7.5, 37°C | Homo sapiens | |
0.032 | - |
Abz-VKMDAE-EDDnp | pH 7.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | meprin beta is a type I transmembrane protein | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | metalloprotease | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amyloid precursor protein + H2O | Homo sapiens | meprin beta can also process amyloid precursor protein in a manner reminiscent of beta-secretase, identification of cleavage sites of meprin beta in the amyloid beta sequence of the wild-type and Swedish mutant of amyloid precursor protein at positions p1 and p2, thereby generating amyloid beta variants starting at the first or second amino acid residue, mass spectrometry analysis, overview | amyloid precursor protein N-terminal fragments + amyloid beta protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16820 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | meprin beta mRNA levels are increased in brains of Alzheimer's disease patients compared with age-matched control individuals | Homo sapiens | - |
additional information | wide expression pattern | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-VKMDAE-EDDnp + H2O | wild-type beta-site fluorogenic substrate sequence | Homo sapiens | ? | - |
? | |
Abz-VNLDAE-EDDnp + H2O | Swedish mutant beta-site fluorogenic substrate sequence | Homo sapiens | ? | - |
? | |
amyloid precursor protein + H2O | meprin beta can also process amyloid precursor protein in a manner reminiscent of beta-secretase, identification of cleavage sites of meprin beta in the amyloid beta sequence of the wild-type and Swedish mutant of amyloid precursor protein at positions p1 and p2, thereby generating amyloid beta variants starting at the first or second amino acid residue, mass spectrometry analysis, overview | Homo sapiens | amyloid precursor protein N-terminal fragments + amyloid beta protein | - |
? | |
amyloid precursor protein + H2O | catalytic properties and cleavage sites of meprin beta within different amyloid precursor protein peptides, overview | Homo sapiens | amyloid precursor protein N-terminal fragments + amyloid beta protein | - |
? | |
amyloid precursor protein APP751 + H2O | recombinant human substrate, coexpressed with the enzyme in HEK-293T cells, or exogenous soluble meprin beta incubated with APP751 stably overexpressing 7WD10 cells, is able to produce amyloid precursor fragment N-APP20 | Homo sapiens | amyloid precursor protein N-terminal fragments + amyloid beta protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
meprin beta | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
Abz-VKMDAE-EDDnp | pH 7.5, 37°C | Homo sapiens | |
20.9 | - |
Abz-VNLDAE-EDDnp | pH 7.5, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the astacin protease family | Homo sapiens |
physiological function | processing of the amyloid precursor protein is of critical importance, the enzyme cleaves amyloid precursor protein and liberates soluble N-terminal amyloid precursor protein fragments | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
480 | - |
Abz-VKMDAE-EDDnp | pH 7.5, 37°C | Homo sapiens | |
1410 | - |
Abz-VNLDAE-EDDnp | pH 7.5, 37°C | Homo sapiens |