3.4.24.28: bacillolysin

This is an abbreviated version!
For detailed information about bacillolysin, go to the full flat file.

Reaction

similar, but not identical, to that of thermolysin =

Synonyms

Anilozyme P 10, bacillolysin, bacillolysin-neutral protease, Bacillus metalloendopeptidase, Bacillus metalloproteinase, Bacillus neutral proteinase, Bacillus polymyxa protease, Bacillus pumilus metalloendopeptidase, Bacillus subtilis neutral protease, Bacillus subtilis neutral proteinase, Bae16, BL-MA, BMP1, C histolyticum neutral protease, CHNP, EC 3.4.24.4, MCP 76, Megateriopeptidase, metallo-neutral protease, MprBi, mprBp, Neutral protease, neutrase, non-specific neutral protease, protease N, thermolysin-like enzyme, Thermostable neutral protease, thermoysin-like proteinase, TLP-ste, TLP-sub

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.28 bacillolysin

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Results	in table
3	Activating Compound
150	AA Sequence
6	Application
1	CAS Registry Number
9	Cloned(Commentary)
2	Crystallization (Commentary)
2	Expression
7	General Information
4	General Stability
42	Inhibitors
3	Ki Value [mM]
8	KM Value [mM]
5	Localization
21	Metals/Ions
15	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
12	Organic Solvent Stability
44	Organism
1	PDB ID
8	pH Optimum
7	pH Range
11	pH Stability
1	pI Value
6	Protein Variants
12	Purification (Commentary)
1	Reaction
1	Reaction Type
44	Reference
2	Renatured (Commentary)
6	Source Tissue
22	Specific Activity [micromol/min/mg]
81	Substrates and Products (Substrate)
11	Subunits
39	Synonyms
17	Temperature Optimum [°C]
5	Temperature Range [°C]
17	Temperature Stability [°C]
6	Turnover Number [1/s]

Purification

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Purification on EC 3.4.24.28 - bacillolysin

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

2 entries

after expression in Escherichia coli with N-terminal His-tag

Geobacillus stearothermophilus

-

670771

extracellular enzyme from culture medium by anion exchange chromatography

Paenibacillus polymyxa

-

718012

extracellular enzyme from culture medium by several chromatographic steps including one affinity based step

Hathewaya histolytica

-

718012

native enzyme 13.1fold by ethanol precipitation, gel filtration, and anion exchange chromatography

Bacillus cereus

-

733469

native extracellular enzyme 5.6fold from culture supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration

Bacillus amyloliquefaciens

-

733130

recombinant enzyme

Geobacillus stearothermophilus

P43133

701210

recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity

Bacillus intermedius

B3V4Z0

717233

recombinant enzyme from Escherichia coli strain BL21(DE3)

Bacillus thuringiensis

L0AQH5

733167

wild-type and mutant enzymes

Geobacillus stearothermophilus

-

651101

wild-type and mutant TLPs

Bacillus subtilis

-

650655

-

Geobacillus stearothermophilus

-

31163

-

Bacillus cereus

-

31159