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3.4.24.28: bacillolysin

This is an abbreviated version!
For detailed information about bacillolysin, go to the full flat file.

Word Map on EC 3.4.24.28

Reaction

similar, but not identical, to that of thermolysin =

Synonyms

Anilozyme P 10, bacillolysin, bacillolysin-neutral protease, Bacillus metalloendopeptidase, Bacillus metalloproteinase, Bacillus neutral proteinase, Bacillus polymyxa protease, Bacillus pumilus metalloendopeptidase, Bacillus subtilis neutral protease, Bacillus subtilis neutral proteinase, Bae16, BL-MA, BMP1, C histolyticum neutral protease, CHNP, EC 3.4.24.4, MCP 76, Megateriopeptidase, metallo-neutral protease, MprBi, mprBp, Neutral protease, neutrase, non-specific neutral protease, protease N, thermolysin-like enzyme, Thermostable neutral protease, thermoysin-like proteinase, TLP-ste, TLP-sub

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.28 bacillolysin

Cloned

Cloned on EC 3.4.24.28 - bacillolysin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
-
expression in Bacillus subtilis
gene bmp1, recombinant expression in Escherichia coli strain BL21(DE3)
gene contains a large open reading frame between the regions coding for signal sequence and mature protein
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gene mprBi, DNA and amino acid sequence determination and analysis, enzyme expression in Bacillus subtilis and secretion to the culture medium
nucleotide sequence and promoter region
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protease-deficient strain Bacillus subtilis DB117 used as host for plasmids, subcloned in Escherichia coli for site-directed mutagenesis
-