3.4.24.28: bacillolysin

This is an abbreviated version!
For detailed information about bacillolysin, go to the full flat file.

Reaction

similar, but not identical, to that of thermolysin =

Synonyms

Anilozyme P 10, bacillolysin, bacillolysin-neutral protease, Bacillus metalloendopeptidase, Bacillus metalloproteinase, Bacillus neutral proteinase, Bacillus polymyxa protease, Bacillus pumilus metalloendopeptidase, Bacillus subtilis neutral protease, Bacillus subtilis neutral proteinase, Bae16, BL-MA, BMP1, C histolyticum neutral protease, CHNP, EC 3.4.24.4, MCP 76, Megateriopeptidase, metallo-neutral protease, MprBi, mprBp, Neutral protease, neutrase, non-specific neutral protease, protease N, thermolysin-like enzyme, Thermostable neutral protease, thermoysin-like proteinase, TLP-ste, TLP-sub

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.24 Metalloendopeptidases

                3.4.24.28 bacillolysin

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Results	in table
3	Activating Compound
150	AA Sequence
6	Application
1	CAS Registry Number
9	Cloned(Commentary)
2	Crystallization (Commentary)
2	Expression
7	General Information
4	General Stability
42	Inhibitors
3	Ki Value [mM]
8	KM Value [mM]
5	Localization
21	Metals/Ions
15	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
12	Organic Solvent Stability
44	Organism
1	PDB ID
8	pH Optimum
7	pH Range
11	pH Stability
1	pI Value
6	Protein Variants
12	Purification (Commentary)
1	Reaction
1	Reaction Type
44	Reference
2	Renatured (Commentary)
6	Source Tissue
22	Specific Activity [micromol/min/mg]
81	Substrates and Products (Substrate)
11	Subunits
39	Synonyms
17	Temperature Optimum [°C]
5	Temperature Range [°C]
17	Temperature Stability [°C]
6	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.4.24.28 - bacillolysin

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10

Bacillus cereus

-

purified enzyme, 60°C, loss of 40% activity after 10 min

733469

11.7

Priestia megaterium

-

60 min, complete loss of activity

669317

4

2 entries

4 - 5

Bacillus amyloliquefaciens

-

purified extracellular enzyme, 50°C, loss of 70% activity after 60 min

733130

5 - 8

Bacillus nematocida

-

stable within this range

667347

5 - 9

Priestia megaterium

-

37°C, 60 min, stable

669317

6

Bacillus amyloliquefaciens

-

purified extracellular enzyme, 50°C, no loss of activity after 60 min, stable at

733130

6 - 9

Bacillus cereus

-

purified enzyme, 60°C, loss of 20-25% activity after 10 min

733469

7

Bacillus amyloliquefaciens

-

purified extracellular enzyme, 50°C, loss of 20% activity after 60 min

733130

8 - 10

Bacillus amyloliquefaciens

-

purified extracellular enzyme, 50°C, loss of 40% activity after 60 min

733130

4

Bacillus nematocida

-

1h, less than 40% remaining activity

667347

4

Priestia megaterium

-

60 min, complete loss of activity

669317