Cloned (Comment) | Organism |
---|---|
gene mprBi, DNA and amino acid sequence determination and analysis, enzyme expression in Bacillus subtilis and secretion to the culture medium | Bacillus intermedius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 94.2% inhibition at 0.5 mM, 100% at 5 mM | Bacillus intermedius | |
Co2+ | - |
Bacillus intermedius | |
Cu2+ | - |
Bacillus intermedius | |
EDTA | 4% inhibition at 0.5 mM, 94.3% at 5 mM | Bacillus intermedius | |
HgCl2 | 49% inhibition at 0.5 mM, 60% at 5 mM | Bacillus intermedius | |
additional information | no inhibition by protein trypsin inhibitor | Bacillus intermedius | |
Ni2+ | - |
Bacillus intermedius | |
PCMB | 6% inhibition at 0.5 mM, 98.1% at 5 mM | Bacillus intermedius | |
PMSF | 6% inhibition at 0.5 mM, 9% at 5 mM | Bacillus intermedius | |
Zn2+ | secreted zinc-dependent endopeptidase, addition of exogenous Zn2+ at 0.01 mM inhibits the enzyme | Bacillus intermedius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
azocasein | recombinant wild-type enzyme, pH 8.0, 37°C | Bacillus intermedius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted zinc-dependent endopeptidase | Bacillus intermedius | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 30% activation at 10 mM | Bacillus intermedius | |
Mg2+ | 20% activation at 10 mM | Bacillus intermedius | |
Zn2+ | secreted zinc-dependent endopeptidase, addition of exogenous Zn2+ at 0.01 mM inhibits the enzyme | Bacillus intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
x * 19000, SDS-PAGE | Bacillus intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus intermedius | B3V4Z0 | gene mprBi | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity | Bacillus intermedius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0057 | - |
with substrate Dnp-AAVR, pH 8.0, 37°C | Bacillus intermedius |
0.0145 | - |
with substrate Dnp-GGLR, pH 8.0, 37°C | Bacillus intermedius |
0.0264 | - |
with substrate Dnp-GGK, pH 8.0, 37°C | Bacillus intermedius |
0.0282 | - |
with substrate Dnp-GGIR, pH 8.0, 37°C | Bacillus intermedius |
0.0365 | - |
with substrate Dnp-GGFR, pH 8.0, 37°C | Bacillus intermedius |
0.0515 | - |
with substrate Dnp-AALR-NH2, pH 8.0, 37°C | Bacillus intermedius |
17.4 | - |
with substrate azocasein, recombinant purified wild-type enzyme, pH 8.0, 37°C | Bacillus intermedius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Bacillus intermedius | ? | - |
? | |
Dnp-AALR-NH2 + H2O | - |
Bacillus intermedius | ? | - |
? | |
Dnp-AAVR + H2O | - |
Bacillus intermedius | ? | - |
? | |
Dnp-GGFR + H2O | - |
Bacillus intermedius | ? | - |
? | |
Dnp-GGIR + H2O | - |
Bacillus intermedius | ? | - |
? | |
Dnp-GGK + H2O | - |
Bacillus intermedius | ? | - |
? | |
Dnp-GGLR + H2O | - |
Bacillus intermedius | ? | - |
? | |
additional information | analysis of substrate specificity of MprBi using synthetic chromogenic substrates, MALDI-TOF mass spectrometric product analysis, overview | Bacillus intermedius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 19000, SDS-PAGE | Bacillus intermedius |
Synonyms | Comment | Organism |
---|---|---|
MprBi | - |
Bacillus intermedius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus intermedius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 70 | activity range | Bacillus intermedius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1210 | - |
azocasein | recombinant wild-type enzyme, pH 8.0, 37°C | Bacillus intermedius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
asay at | Bacillus intermedius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus intermedius | - |
- |
5.4 |
General Information | Comment | Organism |
---|---|---|
evolution | MprBi is a metzincin clan adamalysin/reprolysin-like metalloprotease and belongs to the class of zinc-dependent metalloproteinases | Bacillus intermedius |