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Literature summary for 3.4.24.28 extracted from

  • Rudakova, N.L.; Balaban, N.P.; Danilova, Y.V.; Rudenskaya, G.N.; Sharipova, M.R.
    Characteristics of a novel secreted zinc-dependent endopeptidase of Bacillus intermedius (2010), Biochemistry (Moscow), 75, 1294-1301.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mprBi, DNA and amino acid sequence determination and analysis, enzyme expression in Bacillus subtilis and secretion to the culture medium Bacillus intermedius

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 94.2% inhibition at 0.5 mM, 100% at 5 mM Bacillus intermedius
Co2+
-
Bacillus intermedius
Cu2+
-
Bacillus intermedius
EDTA 4% inhibition at 0.5 mM, 94.3% at 5 mM Bacillus intermedius
HgCl2 49% inhibition at 0.5 mM, 60% at 5 mM Bacillus intermedius
additional information no inhibition by protein trypsin inhibitor Bacillus intermedius
Ni2+
-
Bacillus intermedius
PCMB 6% inhibition at 0.5 mM, 98.1% at 5 mM Bacillus intermedius
PMSF 6% inhibition at 0.5 mM, 9% at 5 mM Bacillus intermedius
Zn2+ secreted zinc-dependent endopeptidase, addition of exogenous Zn2+ at 0.01 mM inhibits the enzyme Bacillus intermedius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
azocasein recombinant wild-type enzyme, pH 8.0, 37°C Bacillus intermedius

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted zinc-dependent endopeptidase Bacillus intermedius
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 30% activation at 10 mM Bacillus intermedius
Mg2+ 20% activation at 10 mM Bacillus intermedius
Zn2+ secreted zinc-dependent endopeptidase, addition of exogenous Zn2+ at 0.01 mM inhibits the enzyme Bacillus intermedius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
x * 19000, SDS-PAGE Bacillus intermedius

Organism

Organism UniProt Comment Textmining
Bacillus intermedius B3V4Z0 gene mprBi
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity Bacillus intermedius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0057
-
with substrate Dnp-AAVR, pH 8.0, 37°C Bacillus intermedius
0.0145
-
with substrate Dnp-GGLR, pH 8.0, 37°C Bacillus intermedius
0.0264
-
with substrate Dnp-GGK, pH 8.0, 37°C Bacillus intermedius
0.0282
-
with substrate Dnp-GGIR, pH 8.0, 37°C Bacillus intermedius
0.0365
-
with substrate Dnp-GGFR, pH 8.0, 37°C Bacillus intermedius
0.0515
-
with substrate Dnp-AALR-NH2, pH 8.0, 37°C Bacillus intermedius
17.4
-
with substrate azocasein, recombinant purified wild-type enzyme, pH 8.0, 37°C Bacillus intermedius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Bacillus intermedius ?
-
?
Dnp-AALR-NH2 + H2O
-
Bacillus intermedius ?
-
?
Dnp-AAVR + H2O
-
Bacillus intermedius ?
-
?
Dnp-GGFR + H2O
-
Bacillus intermedius ?
-
?
Dnp-GGIR + H2O
-
Bacillus intermedius ?
-
?
Dnp-GGK + H2O
-
Bacillus intermedius ?
-
?
Dnp-GGLR + H2O
-
Bacillus intermedius ?
-
?
additional information analysis of substrate specificity of MprBi using synthetic chromogenic substrates, MALDI-TOF mass spectrometric product analysis, overview Bacillus intermedius ?
-
?

Subunits

Subunits Comment Organism
? x * 19000, SDS-PAGE Bacillus intermedius

Synonyms

Synonyms Comment Organism
MprBi
-
Bacillus intermedius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bacillus intermedius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 70 activity range Bacillus intermedius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1210
-
azocasein recombinant wild-type enzyme, pH 8.0, 37°C Bacillus intermedius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
asay at Bacillus intermedius

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus intermedius
-
-
5.4

General Information

General Information Comment Organism
evolution MprBi is a metzincin clan adamalysin/reprolysin-like metalloprotease and belongs to the class of zinc-dependent metalloproteinases Bacillus intermedius