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Literature summary for 3.4.24.28 extracted from

  • Zhang, M.; Zhao, C.; Du, L.; Lu, F.; Gao, C.
    Expression, purification, and characterization of a thermophilic neutral protease from Bacillus stearothermophilus in Bacillus subtilis (2008), Sci. China C Life Sci., 51, 52-59.
    View publication on PubMed
Search for more literature for 3.4.24.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus P43133
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 16530 microg Tyr produced per min, pH 7.5, 65°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Geobacillus stearothermophilus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 1 h, 80% residual activity Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 sharp decrease in activity below Geobacillus stearothermophilus
6.5 9
-
 Geobacillus stearothermophilus