Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.23.18: Aspergillopepsin I

This is an abbreviated version!
For detailed information about Aspergillopepsin I, go to the full flat file.

Word Map on EC 3.4.23.18

Reaction

hydrolysis of proteins with broad specificity. Generally favours hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk =

Synonyms

A. protease, Acid protease, ACP, AOAP, AP, APP, Asp f 13, Asp f 15, aspartic protease, aspartic protease P6281, Aspartic proteinase, aspergillopepsin, Aspergillopepsin A, Aspergillopepsin F, aspergillopepsin I, aspergillopepsin O, Aspergillopeptidase A, Aspergillus acid protease, Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, Aspergillus carboxyl proteinase, Aspergillus niger acid proteinase, Aspergillus protease, Aspergillus saitoi acid proteinase, Avamorin, Awamorin, carboxyl protease, Carboxyl proteinase, carboxyl proteinase I, Denapsin, denapsin 2P, Denapsin XP 271, EC 3.4.23.6, extracellular aspartic protease, More, PepA, PEPO, Pepsin-type aspartic proteinase, proApp, Proctase, Proctase B, Proctase P, Proteinase B, Proteinase, Aspergillus acid, Proteinase, Aspergillus awamori acid, Proteinase, Aspergillus kawachii aspartic, Proteinase, Aspergillus saitoi acid, Sumizyme AP, Trypsinogen kinase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.18 Aspergillopepsin I

Temperature Stability

Temperature Stability on EC 3.4.23.18 - Aspergillopepsin I

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 25
-
pH 2.7, 16 h stable
20 - 45
after incubation at 20°C for 1 h, the enzyme with propeptide retains 100% activity. 38.1°C is the melting temperature of the catalytically active enzyme with propeptide. When the temperature is over 45°C, the relative enzyme activities of the enzyme with propeptide decreases to below 40%
30
-
pH 7, drastic decrease of activity probably due to autolytic activity
30 - 50
the enzyme maintains 39.8% of its maximum activity after 5 min incubation at 50°C. The protease remains stable after 1 h of incubation at 30 and 35°C, retaining more than 70% activity
40
-
below, stable
50 - 80
-
the enzyme is almost 100 % stable at 50°C for 24 h. Retention of full activity is seen for over 4, 2, 1 h and 30 min at 50, 60, 70 and 80°C, respectively. However, a loss in relative activity in the range of 1.3 % (50°C) to 19.6 % (at 80°C) is noted after 24 h
60
-
20 min, at pH 2.8, complete inactivation