Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme activity remains unaltered in the presence of H2O2 and NaClO | Aspergillus terreus | |
SDS | - |
Aspergillus terreus | |
Triton-X100 | - |
Aspergillus terreus | |
Tween-80 | - |
Aspergillus terreus |
General Stability | Organism |
---|---|
the protease is 100 % stable and compatible for 2 h at 60°C with all the detergents tested except for Super wheel, and a retention of 83.98, 85.50, 85.57, 87.14 and 89.16 % is recorded after 24 h for Super wheel, More choice, Ariel, Henko and Surf excel, respectively | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DAN | 91.7% residual activity at 5 mM | Aspergillus terreus | |
dithiothreitol | 90.1% residual activity at 5 mM | Aspergillus terreus | |
EDTA | 87.3% residual activity at 5 mM | Aspergillus terreus | |
Fe3+ | 81.1% residual activity at 10 mM | Aspergillus terreus | |
Hg2+ | 74.8% residual activity at 10 mM | Aspergillus terreus | |
IAA | 94.8% residual activity at 5 mM | Aspergillus terreus | |
K+ | 97.4% residual activity at 10 mM | Aspergillus terreus | |
Mn2+ | 85.9% residual activity at 10 mM | Aspergillus terreus | |
additional information | not inhibited by Fe2+ and Cu2+ | Aspergillus terreus | |
N-ethylmaleimide | 88.8% residual activity at 5 mM | Aspergillus terreus | |
Na+ | 92.54% residual activity at 10 mM | Aspergillus terreus | |
NaN3 | 78% residual activity at 5 mM | Aspergillus terreus | |
NBS | 49.7% residual activity at 5 mM | Aspergillus terreus | |
phenylmethylsulfonyl fluoride | 16.3% residual activity at 5 mM | Aspergillus terreus | |
TLCK | 93.3% residual activity at 5 mM | Aspergillus terreus | |
Urea | 95.5% residual activity at 5 mM | Aspergillus terreus | |
Zn2+ | 89.1% residual activity at 10 mM | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 182.6% activity at 10 mM | Aspergillus terreus | |
Co2+ | 121.1% activity at 10 mM | Aspergillus terreus | |
Mg2+ | 152.2% activity at 10 mM | Aspergillus terreus | |
Na+ | 102.88% activity at 10 mM | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Aspergillus terreus gr. | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is 100 % stable in the presence of bleaching and oxidizing agents | Aspergillus terreus |
Purification (Comment) | Organism |
---|---|
concanavalin A-agarose column chromatography | Aspergillus terreus |
Storage Stability | Organism |
---|---|
4, 7 and 9% of the enzyme's initial activity are lost after 40 days at 4, 28 and -20°C, respectively | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
casein + H2O | highest activity | Aspergillus terreus | ? | - |
? | |
casein + H2O | highest activity | Aspergillus terreus gr. | ? | - |
? | |
Gelatin + H2O | - |
Aspergillus terreus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16000, SDS-PAGE | Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | the enzyme is almost 100 % stable at 50°C for 24 h. Retention of full activity is seen for over 4, 2, 1 h and 30 min at 50, 60, 70 and 80°C, respectively. However, a loss in relative activity in the range of 1.3 % (50°C) to 19.6 % (at 80°C) is noted after 24 h | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Aspergillus terreus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 12 | the enzyme is active over a broad alkaline pH range of 8.0-12.0 | Aspergillus terreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 12 | the protease demonstrates excellent stability at pH range 8.0-12.0 for 30 min. The enzyme remains unaltered at pH 11.0 and 12.0 for 24 h at 28°C and a slight decrease in relative activity is seen at pH 10.0 after 24 h | Aspergillus terreus |