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3.4.23.18: Aspergillopepsin I

This is an abbreviated version!
For detailed information about Aspergillopepsin I, go to the full flat file.

Word Map on EC 3.4.23.18

Reaction

hydrolysis of proteins with broad specificity. Generally favours hydrophobic residues in P1 and P1', but also accepts Lys in P1, which leads to activation of trypsinogen. Does not clot milk =

Synonyms

A. protease, Acid protease, ACP, AOAP, AP, APP, Asp f 13, Asp f 15, aspartic protease, aspartic protease P6281, Aspartic proteinase, aspergillopepsin, Aspergillopepsin A, Aspergillopepsin F, aspergillopepsin I, aspergillopepsin O, Aspergillopeptidase A, Aspergillus acid protease, Aspergillus acid proteinase, Aspergillus aspartic proteinase, Aspergillus awamori acid proteinase, Aspergillus carboxyl proteinase, Aspergillus niger acid proteinase, Aspergillus protease, Aspergillus saitoi acid proteinase, Avamorin, Awamorin, carboxyl protease, Carboxyl proteinase, carboxyl proteinase I, Denapsin, denapsin 2P, Denapsin XP 271, EC 3.4.23.6, extracellular aspartic protease, More, PepA, PEPO, Pepsin-type aspartic proteinase, proApp, Proctase, Proctase B, Proctase P, Proteinase B, Proteinase, Aspergillus acid, Proteinase, Aspergillus awamori acid, Proteinase, Aspergillus kawachii aspartic, Proteinase, Aspergillus saitoi acid, Sumizyme AP, Trypsinogen kinase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.18 Aspergillopepsin I

Purification

Purification on EC 3.4.23.18 - Aspergillopepsin I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone precipitation
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concanavalin A-agarose column chromatography
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cytoplasmic enzyme: partial
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from crude powder preparation
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from koji culture and submerged cell culture
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from Molsin, a commercial crude enzyme extract
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HisTrap column chromatography and Superdex G-75 gel filtration
Ni2+-Sepharose column chromatography and glutathione Sepharose column chromatography
recombinant enzyme from Escherichia coli after solubilization from inclusion bodies by ion exchange chromatography to homogeneity
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several isoforms (cytoplasmic F1 and F2)
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several isoforms (extracellular A1 and A2)
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several isoforms (membrane-bound M1 and M2)
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the enzyme is purified by 46fold from an Amano protease preparation from Aspergillus oryzae