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Literature summary for 3.4.23.18 extracted from
- Purification and characterization of the two molecular forms of Aspergillus oryzae acid protease (1976), Biochim. Biophys. Acta, 445, 194-204.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| Aspergillus oryzae | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | diisopropyl phosphofluoridate; EDTA; HgCl2, NaF, FeCl3, FeCl2, CoCl2, MgCl2, CaCl2, ZnSO4; monoiodoacetate, 1,10-phenanthroline, 6-aminohexanoate; PCMB | Aspergillus oryzae | |
| N-bromosuccinimide | not | Aspergillus oryzae |  |
| SDS | - |
Aspergillus oryzae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by HgCl2, NaF, FeCl3, FeCl2, CoCl2, MgCl2, CaCl2, ZnSO4 | Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Aspergillus oryzae |
| 32000 | - |
Aspergillus oryzae, enzyme form A2, sedimentation analysis | Aspergillus oryzae |
| 63000 | - |
Aspergillus oryzae, enzyme form A1, sedimetation analysis | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content: less than 0.3% (enzyme form A2), about 50% (enzyme form A1) | Aspergillus oryzae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| several isoforms (extracellular A1 and A2) | Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | Taka-diastase, crude Aspergillus oryzae powder preparation | Aspergillus oryzae | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50 mM sodium acetate, pH 5, stable | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| Cytochrome c + H2O | from horse heart | Aspergillus oryzae | ? | - |
? | |
| Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae | ? | - |
? | |
| Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae | ? | - |
? | |
| Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, in 50 mM sodium acetate buffer, pH 5 | Aspergillus oryzae |
| 55 | - |
isozyme A1 loses 10% of its original activity in contrast to almost complete loss of activity of isozyme A2 | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 3.15 and 3.5 (isozyme A1) | Aspergillus oryzae |
| additional information | - |
pI: 3.9 (isozyme A2) | Aspergillus oryzae |
| 3 | - |
cytochrome c | Aspergillus oryzae |
| 4.2 | - |
urea-denatured hemoglobin | Aspergillus oryzae |
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