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3.4.22.B62: cathepsin L3

This is an abbreviated version!
For detailed information about cathepsin L3, go to the full flat file.

Word Map on EC 3.4.22.B62

Reaction

70fold preference for tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin over typical cathepsin substrates with hydrophobic or aliphatic residues in the S2 position. Efficiently cleaves type I collagen over different pH and temperature conditions =

Synonyms

cathepsin L clade 3, cathepsin L3, CL3, CL3-1, CL3-2, FhCL3, FheCL3, NEJ protein 8, newly excysted juvenile protein 8, SmCL3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B62 cathepsin L3

Turnover Number

Turnover Number on EC 3.4.22.B62 - cathepsin L3

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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.39
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
-
in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C
0.33
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin
-
in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C
0.39
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin
-
-
0.33
benzyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
-
-
0.58
Boc-AGPR-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.01
Boc-VLK-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.033
Boc-VPR-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
6
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
-
in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C
0.0053 - 6
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
0.15
tosyl-GPK-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
1.015
tosyl-GPR-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
1.2
Z-Leu-Arg-NHMe
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.02
Z-Phe-Arg-NHMe
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.03 - 0.33
Z-Val-Leu-Lys-7-amido-4-methylcoumarin
0.043
Z-VVR-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C