3.4.22.B62: cathepsin L3
This is an abbreviated version!
For detailed information about cathepsin L3, go to the full flat file.
Word Map on EC 3.4.22.B62
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3.4.22.B62
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cathepsins
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fasciola
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hepatica
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worm
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schistosoma
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fluke
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peptidases
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schistosomiasis
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mansoni
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adjuvant-free
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japonicum
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samarium
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fasciolosis
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collagenolytic
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drug development
- 3.4.22.B62
- cathepsins
- fasciola
- hepatica
-
worm
- schistosoma
- fluke
- peptidases
- schistosomiasis
- mansoni
-
adjuvant-free
- japonicum
- samarium
-
fasciolosis
-
collagenolytic
- drug development
Reaction
70fold preference for tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin over typical cathepsin substrates with hydrophobic or aliphatic residues in the S2 position. Efficiently cleaves type I collagen over different pH and temperature conditions =
Synonyms
cathepsin L clade 3, cathepsin L3, CL3, CL3-1, CL3-2, FhCL3, FheCL3, NEJ protein 8, newly excysted juvenile protein 8, SmCL3
ECTree
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KM Value
KM Value on EC 3.4.22.B62 - cathepsin L3
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0.02353
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin
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in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C
0.0157
benzyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin
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in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C
0.0096
Boc-AGPR-7-amido-4-methylcoumarin
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.00613
Boc-VLK-7-amido-4-methylcoumarin
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.0211
Boc-VPR-7-amido-4-methylcoumarin
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.0041
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin
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in 50 mM sodium phosphate, pH 6.5, 1 mM dithiothreitol, 1 mM EDTA, at 24°C
0.0098
tosyl-GPK-7-amido-4-methylcoumarin
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.0106
tosyl-GPR-7-amido-4-methylcoumarin
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.048
Z-Leu-Arg-NHMe
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.02005
Z-Phe-Arg-NHMe
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.0117
Z-VVR-7-amido-4-methylcoumarin
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in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C
0.0041
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
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wild type enzyme, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C
0.0052
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
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mutant enzyme W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C
0.0067
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
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mutant enzyme H61N/W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C
0.0157
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wild type enzyme, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C
0.0287
Z-Val-Leu-Lys-7-amido-4-methylcoumarin
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mutant enzyme H61N/W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C
0.0448
Z-Val-Leu-Lys-7-amido-4-methylcoumarin
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mutant enzyme W67L, in 0.1 M sodium phosphate buffer, pH 6.0, 1 mM dithiothreitol and 1 mM EDTA at 25°C