3.4.22.B62: cathepsin L3
This is an abbreviated version!
For detailed information about cathepsin L3, go to the full flat file.
Word Map on EC 3.4.22.B62
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3.4.22.B62
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cathepsins
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fasciola
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hepatica
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worm
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schistosoma
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fluke
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peptidases
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schistosomiasis
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mansoni
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adjuvant-free
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japonicum
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samarium
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fasciolosis
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collagenolytic
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drug development
- 3.4.22.B62
- cathepsins
- fasciola
- hepatica
-
worm
- schistosoma
- fluke
- peptidases
- schistosomiasis
- mansoni
-
adjuvant-free
- japonicum
- samarium
-
fasciolosis
-
collagenolytic
- drug development
Reaction
70fold preference for tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin over typical cathepsin substrates with hydrophobic or aliphatic residues in the S2 position. Efficiently cleaves type I collagen over different pH and temperature conditions =
Synonyms
cathepsin L clade 3, cathepsin L3, CL3, CL3-1, CL3-2, FhCL3, FheCL3, NEJ protein 8, newly excysted juvenile protein 8, SmCL3
ECTree
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Engineering
Engineering on EC 3.4.22.B62 - cathepsin L3
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H61N/W67L
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the double mutant presents a profile very similar to the W67L single mutant
W67L
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the mutation renders the enzyme incapable of digesting collagen. The mutant shows drastic diminution in specificity towards tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin (1440fold) and also large (35fold) decrease in specificity towards Z-Val-Leu-Lys-7-amido-4-methylcoumarin