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3.4.22.B62: cathepsin L3

This is an abbreviated version!
For detailed information about cathepsin L3, go to the full flat file.

Word Map on EC 3.4.22.B62

Reaction

70fold preference for tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin over typical cathepsin substrates with hydrophobic or aliphatic residues in the S2 position. Efficiently cleaves type I collagen over different pH and temperature conditions =

Synonyms

cathepsin L clade 3, cathepsin L3, CL3, CL3-1, CL3-2, FhCL3, FheCL3, NEJ protein 8, newly excysted juvenile protein 8, SmCL3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.B62 cathepsin L3

Engineering

Engineering on EC 3.4.22.B62 - cathepsin L3

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H61N/W67L
-
the double mutant presents a profile very similar to the W67L single mutant
W67L
-
the mutation renders the enzyme incapable of digesting collagen. The mutant shows drastic diminution in specificity towards tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin (1440fold) and also large (35fold) decrease in specificity towards Z-Val-Leu-Lys-7-amido-4-methylcoumarin