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Literature summary for 3.4.22.B62 extracted from

  • Robinson, M.W.; Corvo, I.; Jones, P.M.; George, A.M.; Padula, M.P.; To, J.; Cancela, M.; Rinaldi, G.; Tort, J.F.; Roche, L.; Dalton, J.P.
    Collagenolytic activities of the major secreted cathepsin L peptidases involved in the virulence of the helminth pathogen, Fasciola hepatica (2011), PLoS Negl. Trop. Dis., 5, e1012.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.B62

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia angusta Fasciola hepatica

Inhibitors

Inhibitors Comment Organism Structure
cathepsin K inhibitor II i.e. Z-LNHNHCONHNHLF-Boc Fasciola hepatica
Z-Phe-Ala-CHN2
-
 Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00613
-
 Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0096
-
 Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0098
-
 tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0106
-
 tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0117
-
 Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.02005
-
 Z-Phe-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0211
-
 Boc-VPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.048
-
 Z-Leu-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 x * 24500, mature enzyme, SDS-PAGE Fasciola hepatica
37000
-
 x * 37000, proenzyme, SDS-PAGE Fasciola hepatica

Organism

Organism UniProt Comment Textmining
Fasciola hepatica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column chromatography Fasciola hepatica

Source Tissue

Source Tissue Comment Organism Textmining
larva infective larvae traversing the gut wall secrete cathepsin L3 Fasciola hepatica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-AGPR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Boc-VLK-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Boc-VPR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
additional information the cathepsin L zymogen is autocatalytically activated and processed to mature enzyme by incubation for 2 h at 37°C in 0.1 M sodium citrate buffer (pH 5.0) containing 2 mM dithiothreitol and 2.5 mM EDTA Fasciola hepatica ?
-
 ?
additional information cathepsin L3 readily cleaves substrates with Pro in the P2 position and peptide substrates mimicking the repeating Gly-Pro-Xaa motifs that occur within the primary sequence of collagen Fasciola hepatica ?
-
 ?
tosyl-GPK-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
tosyl-GPR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?
Type I collagen + H2O cathepsin CL3 cleaves at 24 sites within the alpha1 chain and 24 sites within the alpha2 chain of collagen type I Fasciola hepatica ?
-
 ?
type II collagen + H2O cathepsin L3 exhibits collagenase activity by cleaving at multiple sites within the alpha1 and alpha2 triple helix regions (Col domains) Fasciola hepatica ?
-
 ?
Z-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
 Fasciola hepatica Z-Gly-Pro + Gly + Gly + L-Pro-L-Ala
-
 ?
Z-Gly-Pro-Leu-Gly-Pro + H2O
-
 Fasciola hepatica Z-Gly-Pro + L-Leu + Gly + L-Pro
-
 ?
Z-Leu-Arg-NHMe + H2O
-
 Fasciola hepatica ?
-
 ?
Z-Phe-Arg-NHMe + H2O
-
 Fasciola hepatica ?
-
 ?
Z-VVR-7-amido-4-methylcoumarin + H2O
-
 Fasciola hepatica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 24500, mature enzyme, SDS-PAGE Fasciola hepatica
? x * 37000, proenzyme, SDS-PAGE Fasciola hepatica

Synonyms

Synonyms Comment Organism
cathepsin L clade 3
-
 Fasciola hepatica
CL3
-
 Fasciola hepatica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
 Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.02
-
 Z-Phe-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.033
-
 Boc-VPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.043
-
 Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.15
-
 tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.58
-
 Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
1.015
-
 tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
1.2
-
 Z-Leu-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000905
-
 cathepsin K inhibitor II in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
0.0003365
-
 Z-Phe-Ala-CHN2 in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.9993
-
 Z-Phe-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
1.564
-
 Boc-VPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
1.748
-
 Boc-VLK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
3.697
-
 Z-VVR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
25
-
 Z-Leu-Arg-NHMe in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
36.42
-
 tosyl-GPK-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
60.76
-
 Boc-AGPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica
95.77
-
 tosyl-GPR-7-amido-4-methylcoumarin in 100 mM sodium phosphate buffer (pH 6.0) containing 1 mM dithiothreitol and 1 mM EDTA, at 37°C Fasciola hepatica