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3.4.22.29: picornain 2A

This is an abbreviated version!
For detailed information about picornain 2A, go to the full flat file.

Word Map on EC 3.4.22.29

Reaction

selective cleavage of Tyr-/-Gly bond in picornavirus polyprotein =

Synonyms

2A cysteine protease, 2A protease, 2A proteinase, 2Apro, coxsackievirus B3 2A protease, CV-B3 proteinase 2A, CVB3 2A, enterovirus 2A protease, EV-A71 2Apro, EV71-2A, HRV 2A protease, HRV16 2A, HRVC15 2Apro, P2A, picornaviral 2A protein, picornaviral 2A proteinase, picornavirus 2A proteinase, picornavirus endopeptidase 2A, poliovirus 2A protease, poliovirus 2Apro, poliovirus protease 2A, poliovirus protease 2Apro, protease 2A, proteinase 2A, proteinase 2Apro, proteinase, poliovirus, 2A, PV 2Apro, rhinovirus protease 2A, ubiquitin-specific protease 2a, USP2a, Y-G proteinase 2A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.29 picornain 2A

Cloned

Cloned on EC 3.4.22.29 - picornain 2A

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a chimeric protein (maltose-binding protein-2Apro) is expressed
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coxsackievirus B4
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expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli BL21(DE3)pLysS
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expressed in Escherichia coli Rosetta cells
expressed in Escherichia coli strains Rosetta2(DE3)-pLysS and BL21-DE3 CodonPlus RILP
expressed in HeLa cells
expressed in Huh7-T7 cells
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expressed in Mus musculus cardiac myocytes
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expression in Escherichia coli
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human rhinovirus type 2
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in vitro transcription of transcript RNAs from the mono-cistronic reporter plasmid, the bi-cistronic reporter plasmid, the wild type EV-A71 replicon, the EV-A71 AITTL deleted replicon, the EV-A71 2Apro mutated replicon, and the EV-A71 2Apro deleted replicon
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recombinant expression of GFP-tagged or mCherry-tagged enzyme in Escherichia coli
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recombinant expression of wild-type enzyme and mutant 2AC110S in HeLa cells
sequence comparisons, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)
to test whether picornaviral 2A sequences can be used to express foreign genes in adenoviruses, 2A skipping site are inserted after the protein IX gene in an oncolytic virus that targets colon cancer cells
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various reporter plasmids are transfected into recombinant vaccinia virus-infected BHK cells alone or with the plasmid pGEM3Z/J1, which expresses the swine vesicular disease virus 2A protease, the plasmids are transfected using FuGene6 into cells previously infected with the vaccinia virus vTF7-3, which expresses the T7 RNA polymerase
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wild-type and mutant picornaviruses are isolated from HeLa cells transfected with in vitro-synthesized RNA using DEAE-dextran, RNA transcripts are produced from a picornavirus type 1 Mahoney P1M infectious clone, pT7M, or a picornavirus type 2 Lansing (P2L) infectious clone, pT7L
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