3.4.22.29: picornain 2A
This is an abbreviated version!
For detailed information about picornain 2A, go to the full flat file.
Word Map on EC 3.4.22.29
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3.4.22.29
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polyprotein
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enteroviruses
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eif4gi
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capsid
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picornaviruses
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foot-and-mouth
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enteroviral
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3cpro
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shutoff
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rhinoviruses
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myocarditis
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cap-independent
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deubiquitinating
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poliovirus-infected
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encephalomyocarditis
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picornaviridae
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cap-binding
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eif-4f
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ires-driven
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dicistronic
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polioviruses
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ires-dependent
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2a-mediated
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rhinoviral
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polya-binding
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molecular biology
- 3.4.22.29
- polyprotein
- enteroviruses
- eif4gi
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capsid
- picornaviruses
- foot-and-mouth
-
enteroviral
- 3cpro
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shutoff
- rhinoviruses
- myocarditis
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cap-independent
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deubiquitinating
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poliovirus-infected
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encephalomyocarditis
- picornaviridae
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cap-binding
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eif-4f
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ires-driven
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dicistronic
- polioviruses
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ires-dependent
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2a-mediated
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rhinoviral
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polya-binding
- molecular biology
Reaction
selective cleavage of Tyr-/-Gly bond in picornavirus polyprotein =
Synonyms
2A cysteine protease, 2A protease, 2A proteinase, 2Apro, coxsackievirus B3 2A protease, CV-B3 proteinase 2A, CVB3 2A, enterovirus 2A protease, EV-A71 2Apro, EV71-2A, HRV 2A protease, HRV16 2A, HRVC15 2Apro, P2A, picornaviral 2A protein, picornaviral 2A proteinase, picornavirus 2A proteinase, picornavirus endopeptidase 2A, poliovirus 2A protease, poliovirus 2Apro, poliovirus protease 2A, poliovirus protease 2Apro, protease 2A, proteinase 2A, proteinase 2Apro, proteinase, poliovirus, 2A, PV 2Apro, rhinovirus protease 2A, ubiquitin-specific protease 2a, USP2a, Y-G proteinase 2A
ECTree
3.4.22.29 picornain 2AAdvanced search results
results (
results (Natural Substrates Products
Natural Substrates Products on EC 3.4.22.29 - picornain 2A
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NATURAL SUBSTRATE 
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cellular eukaryotic translation initiation factor eIF4GI + H2O
peptides
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-
-
-
?
cellular eukaryotic translation initiation factor eIF4GII + H2O
peptides
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-
-
-
?
Grb2-associated binding protein 1 + H2O
?
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GAB1 is cleaved during CV-B3 infection by viral proteinase 2A
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-
?
Grb2-associated binding protein 2 + H2O
GAB2-N1-237 + GAB2-C238-676
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GAB2 is cleaved at G238 during CV-B3 infection by viral proteinase 2A, generating two cleaved fragments of GAB2-N1-237 and GAB2-C238-676
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-
?
HRV 3Cpro precursor 3CD + H2O
3Cpro + 3Dpro
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activation of 3C protease, EC 3.4.22.28, of human rhinovirus A-16
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-
?
human eukaryotic translation initiation factor 4 G I + H2O
?
human eIF4GI
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-
?
human eukaryotic translation initiation factor 4 G II + H2O
?
human eIF4GII
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-
?
nuclear factor of activated T cells 5/tonicity enhancer binding protein
?
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NFAT5/TonEBP, a cellular transcription factor, the protein is cleaved by CVB3 protease 2A at Gly503, inactivation of NFAT5
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-
?
picornavirus polyprotein + H2O
?
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intramolecular reaction, processing begins before synthesis of the polyprotein is complete, cleavage separates capsid protein precursor and noncapsid protein precursor
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-
?
poly(A) binding protein + H2O
fragments of poly(A) binding protein
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contains 1 cleavage site for 2A proteinase within the proline-rich linker domain
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-
?
cytokeratin 8 + H2O
?
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the cleavage results in removal of 14 amino acids from the N-terminal head domain of cytokeratin 8. Cleavage occurs late in the infection cycle at the time of the onset of the cytopathic effect
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-
?
dystrophin + H2O
?
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the protease cleaves dystrophin in coxsackievirus B3-infected myocytes. The cleavage functionally impairs dystrophin
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-
?
dystrophin + H2O
?
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functional impairment and morphological disruption of dystrophin
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-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
-
turns off host-cell protein synthesis
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-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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involved in shut-off in translation of cellular mRNAs upon viral infection, induces cleavage of eukaryotic initiation factor (eIF)4gamma component (i.e. p220) of eIF-4, formerly eIF-4F
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-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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turns off host-cell protein synthesis
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-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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turns off host-cell protein synthesis
-
-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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turns off host-cell protein synthesis
-
-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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turns off host-cell protein synthesis
-
-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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involved in shut-off in translation of cellular mRNAs upon viral infection, induces cleavage of eukaryotic initiation factor (eIF)4gamma component (i.e. p220) of eIF-4, formerly eIF-4F
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-
?
eukaryotic translation initiation factor 4F p220 subunit + H2O
?
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turns off host-cell protein synthesis
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-
?
eukaryotic translation initiation factor 4G + H2O
?
eIF4G
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-
?
poliovirus polypeptide + H2O
?
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involved in primary processing of poliovirus
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-
?
poliovirus polypeptide + H2O
?
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involved in primary processing of poliovirus
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-
?
poliovirus polypeptide + H2O
?
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involved in primary processing of poliovirus
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-
?
poliovirus polypeptide + H2O
?
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immediately after polypeptide 2A has been synthesized the Tyr-Gly pair between P1 and P2 of the nascent chain is cleaved intramolecularly
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-
?
poliovirus polypeptide + H2O
?
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involved in primary processing of poliovirus
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-
?
additional information
?
-
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2Apro cleaves eIF4G, separating the domains of eIF4GI or eIF4GII that bind to eIF4E (and mRNA) and eIF3 (and the 40S ribosome) and inhibits de novo translation initiation by interfering with the ribosome mRNA binding step
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-
?
additional information
?
-
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DAP5 is cleaved during CVB3 infection in tissue culture and in mouse heart
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-
?
additional information
?
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in vivo assays by expression of GAB2 proteins in virus-infected HeLa cells
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-
?
additional information
?
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enzyme, or its polyprotein precursors, are required to stabilize viral RNA and prolong translation. Enzyme activity stimulates negative-strand initiation by approximately fivefold, but has no effect on stability. Stimulation of negative-strand synthesis is independent of the effect on stability and translation
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-
?
additional information
?
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2Apro processes the viral polyprotein, and it cleaves a variety of host proteins, including the translation proteins eIF4GI, eIF4GII and poly(A)-binding protein
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-
?
additional information
?
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important cellular substrate for 2Apro is eIF4G, cleavage of this translation protein leads to inhibition of host protein synthesis during picornavirus infection
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?
additional information
?
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inhibitory role for 2Apro in the most downstream event in interferon signaling, the antiviral activities of interferon-stimulated genes
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-
?
additional information
?
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picornaviral 2A sequences are used to express transgenes in oncolytic adenoviruses
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-
?
additional information
?
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proteinase 2Apro is essential for enterovirus replication in type I interferon-treated cells
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-
?
additional information
?
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ribosome skipping is a mechanism that allows translation of multiple proteins from a single mRNA, it is based on the use of a picornaviral 2A sequence that causes the ribosome to continue translation after skipping the formation of one peptide bond
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?
