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3.4.22.29: picornain 2A

This is an abbreviated version!
For detailed information about picornain 2A, go to the full flat file.

Word Map on EC 3.4.22.29

Reaction

selective cleavage of Tyr-/-Gly bond in picornavirus polyprotein =

Synonyms

2A cysteine protease, 2A protease, 2A proteinase, 2Apro, coxsackievirus B3 2A protease, CV-B3 proteinase 2A, CVB3 2A, enterovirus 2A protease, EV-A71 2Apro, EV71-2A, HRV 2A protease, HRV16 2A, HRVC15 2Apro, P2A, picornaviral 2A protein, picornaviral 2A proteinase, picornavirus 2A proteinase, picornavirus endopeptidase 2A, poliovirus 2A protease, poliovirus 2Apro, poliovirus protease 2A, poliovirus protease 2Apro, protease 2A, proteinase 2A, proteinase 2Apro, proteinase, poliovirus, 2A, PV 2Apro, rhinovirus protease 2A, ubiquitin-specific protease 2a, USP2a, Y-G proteinase 2A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.29 picornain 2A

Purification

Purification on EC 3.4.22.29 - picornain 2A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
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ammonium sulfate precipitation, IMAC resin column chromatography, and Sephacryl S-200 gel filtration
as a fusion protein
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Coxsackievirus B3 2Apro is purified from pET-Cx2A using ion-exchange chromatography and gel filtration
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expression in Escherichia coli
from infected HeLa cells
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from infected HeLa cells (type S3 cells)
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glutathione-Sepharose 4B column chromatography, Ni-NTA spin column chromatography, MonoQ column chromatography, and HiLoad Superdex gel filtration
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HeLa Ohio cells, 4-5 h after infection, to near homogeneity
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HiTrap Q column chromatography
ion-exchange chromatography (Q-Sepharose)
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Ni-NTA resin column chromatography, Hitrap Q column chromatography, and Superdex 75 gel filtration
recombinant enzyme
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recombinant enzyme as expressed in Escherichia coli BL21(DE3)pLysE bearing pET8c/HRV2 2A
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recombinant enzyme as fusion protein
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recombinant enzyme, as expressed in Escherichia coli BL21(DE3)pLysE bearing pET8c/CVB4 2A
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recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, anion exchange chromatography, and gel filtration
recombinant protein with His-tag, in addition to main protein, detection of degradation product of 14 kDa due to cleavage at G856-V857
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