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3.4.22.29: picornain 2A

This is an abbreviated version!
For detailed information about picornain 2A, go to the full flat file.

Word Map on EC 3.4.22.29

Reaction

selective cleavage of Tyr-/-Gly bond in picornavirus polyprotein =

Synonyms

2A cysteine protease, 2A protease, 2A proteinase, 2Apro, coxsackievirus B3 2A protease, CV-B3 proteinase 2A, CVB3 2A, enterovirus 2A protease, EV-A71 2Apro, EV71-2A, HRV 2A protease, HRV16 2A, HRVC15 2Apro, P2A, picornaviral 2A protein, picornaviral 2A proteinase, picornavirus 2A proteinase, picornavirus endopeptidase 2A, poliovirus 2A protease, poliovirus 2Apro, poliovirus protease 2A, poliovirus protease 2Apro, protease 2A, proteinase 2A, proteinase 2Apro, proteinase, poliovirus, 2A, PV 2Apro, rhinovirus protease 2A, ubiquitin-specific protease 2a, USP2a, Y-G proteinase 2A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.29 picornain 2A

Engineering

Engineering on EC 3.4.22.29 - picornain 2A

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D136N
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
D39E
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
G122E
the mutant exhibits very low cleavage efficiency toward eukaryotic initiation factor 4G
L40F
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
S67F
the mutant shows reduced cleavage efficiency toward eukaryotic initiation factor 4G
V120M
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
Y89L
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
Y90L
the mutant shows reduced cleavage efficiency toward eukaryotic initiation factor 4G
D136N
-
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
-
D39E
-
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
-
G122E
-
the mutant exhibits very low cleavage efficiency toward eukaryotic initiation factor 4G
-
L40F
-
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
-
S67F
-
the mutant shows reduced cleavage efficiency toward eukaryotic initiation factor 4G
-
C110A
inactive
C110S
construction of catalytically inactive enzyme mutant EV71-2AC110S
D136N
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
D144A
the mutant shows increased activity compared to the wild type enzyme
D144A/E145A
inactive
D39E
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
DELTA140-143
inactive
E145A
the mutant shows strongly reduced activity compared to the wild type enzyme
G122E
the mutant exhibits very low cleavage efficiency toward eukaryotic initiation factor 4G
H21N/D39E/C110A
-
site-directed mutagenesis, a EV-A71 2Apro deleted replicon plasmid is constructed by deletion mutagenesis of the wild-type EV-A71 replicon by mutation at three positions (mutations of H21N, D39E, and C110A) of the 2Apro coding sequence
L40F
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
R55A
the mutant shows reduced activity compared to the wild type enzyme
S67F
the mutant shows reduced cleavage efficiency toward eukaryotic initiation factor 4G
V120M
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
Y89L
the mutant shows wild type cleavage efficiency toward eukaryotic initiation factor 4G
Y90L
the mutant shows reduced cleavage efficiency toward eukaryotic initiation factor 4G
C110S
Enterovirus A71 BrCR
-
construction of catalytically inactive enzyme mutant EV71-2AC110S
-
G60R
-
mutant is devoid of eIF4G cleavage activity
134Rstop
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
C101S
-
intramolecular cleavage is reduced to 52% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 20% of that of the wild-type enzyme, cleavage of eIF4G is 75-100% of that of the wild-type enzyme
C106S
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
C112S
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G
C138A
-
intramolecular cleavage is reduced to 90% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 200% of that of the wild-type enzyme, cleavage of eIF4G is 75-100% of that of the wild-type enzyme
C52A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G
C54A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G
C61A
-
intramolecular cleavage is reduced to 54% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 40% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme
D105N
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
D105T
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
D132R/R134D
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
D132T
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
D35A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
D35E
-
intramolecular cleavage is reduced to 16% of the wild-type activity, cleavage of P8-P8' is completely abolished
D35T
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
DELTAG104
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
DELTAG107/108
-
cleavage of P8-P8' is completely abolished
F130L
-
intramolecular cleavage is completely abolished
F130S
-
intramolecular cleavage is completely abolished
F130V
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
F136V
-
intramolecular cleavage is reduced to 57% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 25% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme
G115A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
G115S
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
G118A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
G118S
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
G123A
-
intramolecular cleavage is reduced to 23% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 8% of that of the wild-type enzyme, cleavage of eIF4G is less than 10% of that of the wild-type enzyme
G123A/G124A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
G123S
-
intramolecular cleavage is reduced to 20% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 10% of that of the wild-type enzyme, cleavage of eIF4G is below 10% of that of the wild-type enzyme
G124A
-
intramolecular cleavage is reduced to 22% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 9% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme
G124S
-
intramolecular cleavage is reduced to 30% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 10% of that of the wild-type enzyme, cleavage of eIF4G is 10-25% of that of the wild-type enzyme
H114G
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished, no detectable cleavage of eIF5G
H114N
H135stop
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
H18A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
H18Y
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
K113P/H114A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
N16A
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
R134Q
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished, no cleavage of eIF4G is detected
T121Y
-
intramolecular cleavage and cleavage of P8-P8' is completely abolished
Y289R
-
inactive, substitution of 2Apro A104 can partially restore self-processing on the mutant Y289R
Y289R/A104C
-
substitution of 2Apro A104 can partially restore self-processing on the mutant Y289R
Y289R/A104S
-
substitution of 2Apro A104 can partially restore self-processing on the mutant Y289R
Y85K
-
intramolecular cleavage is reduced to 82% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 39% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme
Y86F
-
intramolecular cleavage is reduced to 62% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 21% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme
Y86K
-
intramolecular cleavage is reduced to 71% of that of the wild-type enzyme and cleavage of P8-P8' is reduced to 34% of that of the wild-type enzyme, cleavage of eIF4G is 25-50% of that of the wild-type enzyme
V54A
-
a BseRI fragment 4742-6148 is transferred from Se1-3C-02 DNA to pT7M, leads to defects in trans but not cis cleavage
Y88L
-
the BstEII fragment of picornavirus DNA from nucleotide 3240-3930 is replaced with a PCR product containing the appropriate mutation, the growth of picornavirus mutant is similar to that of wild-type virus in untreated cells but is completely inhibited in IFN-alpha-treated cells
Y88S
-
the BstEII fragment of picornavirus DNA from nucleotide 3240-3930 is replaced with a PCR product containing the appropriate mutation, the growth of picornavirus mutant is similar to that of wild-type virus in untreated cells but is completely inhibited in IFN-alpha-treated cells
R20D
-
significant defect in self-cleavage activity
R20G
-
significant defect in self-cleavage activity
R20P
-
complete inactivation, no self-cleavage activity
R20V
-
significant defect in self-cleavage activity
additional information