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3.4.22.29: picornain 2A

This is an abbreviated version!
For detailed information about picornain 2A, go to the full flat file.

Word Map on EC 3.4.22.29

Reaction

selective cleavage of Tyr-/-Gly bond in picornavirus polyprotein =

Synonyms

2A cysteine protease, 2A protease, 2A proteinase, 2Apro, coxsackievirus B3 2A protease, CV-B3 proteinase 2A, CVB3 2A, enterovirus 2A protease, EV-A71 2Apro, EV71-2A, HRV 2A protease, HRV16 2A, HRVC15 2Apro, P2A, picornaviral 2A protein, picornaviral 2A proteinase, picornavirus 2A proteinase, picornavirus endopeptidase 2A, poliovirus 2A protease, poliovirus 2Apro, poliovirus protease 2A, poliovirus protease 2Apro, protease 2A, proteinase 2A, proteinase 2Apro, proteinase, poliovirus, 2A, PV 2Apro, rhinovirus protease 2A, ubiquitin-specific protease 2a, USP2a, Y-G proteinase 2A

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.29 picornain 2A

Inhibitors

Inhibitors on EC 3.4.22.29 - picornain 2A

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
3,4-dichloroisocoumarin
-
-
antipain
Aprotinin
-
0.015 mM, 23% inhibition
benzyloxycarbonyl-Ile-Glu-Thr-Asp(OMe)-fluoromethylketone
-
50% inhibition at 0.0077 mM, delay of self-cleavage
benzyloxycarbonyl-LSTL-fluoromethyl ketone
-
IC50: 1050 nM
benzyloxycarbonyl-LSTT-fluoromethyl ketone
-
IC50: 550 nM
benzyloxycarbonyl-Val-Ala-Asp (OMe)-fluoromethyl ketone
-
-
benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone
-
50% inhibition at 0.0056 mM, delay of self-cleavage
benzyloxycarbonyl-Val-Ala-Met-fluoromethyl ketone
-
-
Cd2+
-
inhibits esterase activity
chymostatin
Cl-
-
trace amounts eliminate activity
Co2+
-
0.05 mM
Elastinal
eukaryotic release factor 3
-
increasing concentrations of recombinant His-tagged eRF3 lead to partial inhibition of 2Apro-proteolytic cleavage of poly(A) binding protein that increases modestly
-
Gly-Arg-Thr-Thr-Leu-Ser-Thr-Arg-Gly-Pro-Pro-Arg-Gly-Gly-Pro-Gly
-
-
hinokitiol
-
the cleavage of the cellular eukaryotic translation initiation factor eIF4GI by 2A protease is abolished in the presence of hinokitiol, no significant amounts of eIF4GI cleavage products are found within 24 h of infection
iodoacetamide
leupeptin
LY343813
-
0.025 mM, 61% inhibition
LY343814
-
0.025 mM, 59% inhibition
LY353350
-
0.025 mM, 67% inhibition
N,N,N',N'-tetrakis-(2-pyridylmethyl)-ethylenediamine
-
at 0.005 mM inhibitor, 50% self-processing and eIFGI cleavage are observed at 60 min, whereas the control shows 50% cleavage at 30 min and almost complete cleavage at 60 min
PABP-interacting protein 2
-
The inhibitory effect exerts by PABP-interacting protein 2 is more pronounced on 2Apro, as the lesser concentrations of PABP-interacting protein 2 that leads to partial inhibition of poly(A) binding protein cleavage by 3Cpro results in complete inhibition of 2A-pro-directed cleavage of poly(A) binding protein. 2Apro cleavage is strongly inhibited by in non-ribosome fractions, 40S and 80S ribosomes and polysome fractions.
-
pyrithione
-
the cleavage of the cellular eukaryotic translation initiation factor eIF4GI by 2A protease is abolished in the presence of pyrithione, no significant amounts of eIF4GI cleavage products are found within 24 h of infection
tosyl-L-leucine-chloromethyl ketone
tosyl-L-phenylalanine-chloromethyl ketone
additional information
-