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3,4-dichloroisocoumarin
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Aprotinin
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0.015 mM, 23% inhibition
benzyloxycarbonyl-Ile-Glu-Thr-Asp(OMe)-fluoromethylketone
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50% inhibition at 0.0077 mM, delay of self-cleavage
benzyloxycarbonyl-LSTL-fluoromethyl ketone
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IC50: 1050 nM
benzyloxycarbonyl-LSTT-fluoromethyl ketone
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IC50: 550 nM
benzyloxycarbonyl-Val-Ala-Asp (OMe)-fluoromethyl ketone
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benzyloxycarbonyl-Val-Ala-Asp(OMe)-fluoromethylketone
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50% inhibition at 0.0056 mM, delay of self-cleavage
benzyloxycarbonyl-Val-Ala-Met-fluoromethyl ketone
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Cd2+
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inhibits esterase activity
Cl-
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trace amounts eliminate activity
eukaryotic release factor 3
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increasing concentrations of recombinant His-tagged eRF3 lead to partial inhibition of 2Apro-proteolytic cleavage of poly(A) binding protein that increases modestly
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Gly-Arg-Thr-Thr-Leu-Ser-Thr-Arg-Gly-Pro-Pro-Arg-Gly-Gly-Pro-Gly
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hinokitiol
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the cleavage of the cellular eukaryotic translation initiation factor eIF4GI by 2A protease is abolished in the presence of hinokitiol, no significant amounts of eIF4GI cleavage products are found within 24 h of infection
LY343813
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0.025 mM, 61% inhibition
LY343814
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0.025 mM, 59% inhibition
LY353350
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0.025 mM, 67% inhibition
N,N,N',N'-tetrakis-(2-pyridylmethyl)-ethylenediamine
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at 0.005 mM inhibitor, 50% self-processing and eIFGI cleavage are observed at 60 min, whereas the control shows 50% cleavage at 30 min and almost complete cleavage at 60 min
PABP-interacting protein 2
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The inhibitory effect exerts by PABP-interacting protein 2 is more pronounced on 2Apro, as the lesser concentrations of PABP-interacting protein 2 that leads to partial inhibition of poly(A) binding protein cleavage by 3Cpro results in complete inhibition of 2A-pro-directed cleavage of poly(A) binding protein. 2Apro cleavage is strongly inhibited by in non-ribosome fractions, 40S and 80S ribosomes and polysome fractions.
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pyrithione
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the cleavage of the cellular eukaryotic translation initiation factor eIF4GI by 2A protease is abolished in the presence of pyrithione, no significant amounts of eIF4GI cleavage products are found within 24 h of infection
tosyl-L-leucine-chloromethyl ketone
tosyl-L-phenylalanine-chloromethyl ketone
1,10-phenanthroline
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in trans cleavage activity
antipain
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peptide or eIF cleavage
antipain
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eIF-4gamma as substrate in HeLa cell extracts; human rhinovirus
antipain
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human rhinovirus; peptide or eIF cleavage
Ca2+
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not (10 mM, esterase activity)
chymostatin
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chymostatin
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eIF-4gamma as substrate in HeLa cell extracts; human rhinovirus
chymostatin
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human rhinovirus
Cu2+
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weak, 1 mM, inhibits esterase activity
EDTA
the enzyme is slightly inhibited by 10 mM EDTA
EDTA
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at high concentration
Elastinal
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Elastinal
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eIF-4gamma as substrate in HeLa cell extracts; human rhinovirus
Elastinal
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human rhinovirus
Elastinal
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0.1 mM, 68% inhibition
Hg2+
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iodoacetamide
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-
iodoacetamide
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inhibits esterase activity
iodoacetamide
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1 mM, complete inhibition
leupeptin
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inhibits esterase activity
Mg2+
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not (10 mM, esterase activity)
Mn2+
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10 mM, inhibits esterase activity; weak
NEM
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-
NEM
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inhibits esterase activity
NEM
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1 mM, complete inhibition
tosyl-L-leucine-chloromethyl ketone
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inhibits esterase activity
tosyl-L-leucine-chloromethyl ketone
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-
tosyl-L-leucine-chloromethyl ketone
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1 mM, complete inhibition
tosyl-L-phenylalanine-chloromethyl ketone
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inhibits esterase activity
tosyl-L-phenylalanine-chloromethyl ketone
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weak
Zn2+
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1 mM, inhibits esterase activity
additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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no inhibition by pepstatin (esterase activity); no inhibition by PMSF (esterase activity)
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additional information
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no inhibition by 1,7-phenanthroline and DTT; no inhibition by EGTA
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additional information
drug binding sites include residues His20, Leu21, Asp38, Cys55, Cys57, Met83, Tyr88, Tyr89, Asp108, Cys109, Cys115, His117, Ile123, Thr124, Ala125 and Gly126; drug binding sites include residues His20, Leu21, Asp38, Cys55, Cys57, Met83, Tyr88, Tyr89, Asp108, Cys109, Cyss115, His117, Ile123, Thr124, Ala125 and Gly126
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additional information
drug binding sites include residues His20, Leu21, Asp38, Cys55, Cys57, Met83, Tyr88, Tyr89, Asp108, Cys109, Cys115, His117, Ile123, Thr124, Ala125 and Gly126; drug binding sites include residues His20, Leu21, Asp38, Cys55, Cys57, Met83, Tyr88, Tyr89, Asp108, Cys109, Cyss115, His117, Ile123, Thr124, Ala125 and Gly126
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additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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additional information
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no inhibition by amastatin, bestatin, epiamastatin, foroxymithin, phosphoramidon, Nle-statine-Ala-statine and APMSF; no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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metal-chelating agent
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additional information
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no inhibition by pepstatin
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additional information
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no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64)
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additional information
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no inhibition by unmethylated benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone or benzyloxycarbonyl-Ile-Glu-Thr-Asp-fluoromethylketone
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