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3.4.21.93: Proprotein convertase 1

This is an abbreviated version!
For detailed information about Proprotein convertase 1, go to the full flat file.

Word Map on EC 3.4.21.93

Reaction

release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-/- bonds =

Synonyms

Furin homolog, More, mPC1, mPC1/3, mPC3, mSPC3, murine PC1/3, murine proprotein convertase-1, murine proprotein convertase-1/3, Neuroendocrine convertase 1, PC 1/3, PC1, PC1/3, PC1/PC3, PC3, PCI, PCSK1, prohormone convertase, prohormone convertase 1, prohormone convertase 1/3, Prohormone convertase 3, prohormone convertase PC3, prohormone-convertase 1, propeptide convertase, Propeptide processing protease, proprotein convertase 1, proprotein convertase 1/3, proprotein convertase PC1, proprotein convertase PC1/3, protein convertase 1/3

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.93 Proprotein convertase 1

Cloned

Cloned on EC 3.4.21.93 - Proprotein convertase 1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of PC1/3 and rat pro-islet amyloid polypeptide, proIAPP, in GH3 cells lacking the enzyme, leads to cleavage of recombinant proIAPP
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expressed in Escherichia coli XL1-Blue cells
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expressed in GH4 cells, entire C-terminal tail of PC1 overexpressed in AtT-20 cells
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expression in Escherichia coli
expression in HEK-293 and PC-12 cell
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expression in Spodoptera frugiperda Sf9 cells and Trichoplusioa ni larvae using the baculovirus Autographa californica transfection system, method optimization, modification of the enzyme by C-terminal truncation and exchange of the PC1/3 signal peptide for the glycoprotein gp67 signal peptide, overview
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expression of chimeric PC1-propeptide/SAAS CT peptide constructs in AtT20 cells and in HEK293 cells
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expression of PC1 in GH3 cells, co-expression with proGHRH or preproGHRH, GH3 cells lack endogenous PC1 but contain PC2, EC 3.4.21.94, another prohormone processing enzyme
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expression of PC1 in human 293T cells, leptin has a stimulatory effect on the PC1 pomoter, which is enhanced by co-expression of STAT3, overview
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expression of PC1 using a human PC1 promoter in PC1-deficient GH3 cells, the large region of the PC1 promoter contains negative thyroid hormone response elements which interact with triiodothyronine for regulation of anterior pituitary PC1 mRNA levels, interaction analysis, overview
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expression of wild-type and mutant PC3 in COS-1 cells
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full-length cDNA
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full-length PC3, mutant PC3-deltaTM and truncated forms PC3 (1-638) and PC3 (1-616) expressed in Neuro2A cells
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GH4C1 cells co-infected with vaccinia virus
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hPC1 is expressed in High Five insect cells using a baculovirus protein expression system
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hPC1-luciferase fusion gene expression plasmid
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human PC1 cDNA (amino acids 28-753) amplified, His-tagged and cloned into the MluI and NotI sites of a modified pFASTBAC1 expression vector. Wild-type and mutant N222D human PC1 expressed using baculovirus to infect Sf9 cells
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into pGL3 basic vector to yield wild-type prohormone convertase 1/3 promoter construct, expressed in N29/2 cell line
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mutant expressed in HEK-293 an betaTC3 cells
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PC1 sequenced
Drosophila sp. (in: flies)
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PC1, DNA and amino acid sequence determination and analysis, phylogenetic tree
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PC1/3 produced using the baculovirus expression system in Sf9 insect cells or through intracoelemic injection in insect larvae. PC1/3 CT-peptide from positions 592-726 cloned into a pet24b+ bacterial expression vector. The resulting C-terminally His-tagged protein expressed in Escherichia coli strain BL21 (DE3)
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recombinant enzyme expressed in GH4 cells
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recombinant enzyme expressed in Sf9 cells
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recombinant enzyme expressed in Sf9 cells, mPC1 cDNA inserted intoAutographa californica nuclear polyhedrosis virus, infection of Sf9 cells, mPC1 insert excised from recombinant vaccinia virus vector containg full-length cDNA
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stable expression of PC1 in GH4C1 cells, which lack PC1
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transfection of pituitary GH3 cells with promoter for human prohormone convertase 1/3 or with the prohormone convertase 1/3-minusCRE1/2 promoter construct
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transient enzyme expression in enzyme-deficient rat GH4C1 cells, co-expression with wild-type and mutant proglucagon, glicentin, and/or glicentin-related polypeptide-glucagon, and oxyntomodulin from hamster, overview
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transient expression of proCT construct in HEK293, FD11, CHO-K1, and AtT20 cells, the proCT construct is processed in the secretory pathway
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transplantation of encapsulated PC2-expressing alpha TC-1 cells with PC1/3-expressing alpha TCdeltaPC2 cells in normal mice and low-dose streptozotocin-treated mice
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