3.4.21.91: Flavivirin
This is an abbreviated version!
For detailed information about Flavivirin, go to the full flat file.
Reaction
Selective hydrolysis of -Xaa-Xaa-/-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala
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Synonyms
Dengue NS2b-NS3 protease, Dengue NS3 protease, dengue virus non-structural protein 3, dengue virus NS2B-NS3 protease, dengue virus protease, dengue virus serotype 2 protease, DENV NS2B-NS3, DENV-2 NS2B-NS3 protease, DENV2 NS2BNS3pro, DV2 NS2B/NS3pro, flavivirus NS2B-NS3 protease, non-structural protein 3 serine protease, non-structural protein NS2B-NS3 protease, nonstructural protein 3, NS2-3 protease, NS2B-3 proteinase, NS2B-NS3 complex, NS2B-NS3 protease, NS2B-NS3 protease-helicase, NS2B-NS3 proteinase, NS2B-NS3 proteolytic complex, NS2B-NS3 serine protease, NS2B-NS3 serine proteinase, NS2B-NS3(pro)teinase, NS2b-NS3-protease, NS2B-NS3p, NS2B-NS3pro, NS2B/3 protease, NS2B/NS3, NS2B/NS3 complex, NS2B/NS3 protease, NS2B/NS3 protease complex, NS2B/NS3 proteinase, NS2B/NS3 serine protease, NS2B/NS3pro serine protease, NS2B18NS3, NS2BNS3(pro)tease, NS3, NS3 protease, NS3 proteinase, NS3 serine protease, NS3pro, two-component NS2B-NS3 protease, viral serine protease NS2B/NS3, WNV NS2B-NS3, Yellow fever virus (flavivirus) protease, yellow fever virus NS2B-NS3-181 protease, YFV NS2B/NS3 protease, Zika protease, Zika virus NS2B-NS3 protease, ZIKV NS2B/NS3 protease, ZIKV protease
ECTree
Cofactor
Cofactor on EC 3.4.21.91 - Flavivirin
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NS2B cofactor
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NS2B cofactor
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essential
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NS2B cofactor
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essential
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NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
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NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
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NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
-
NS2B cofactor
N-terminal region of NS3 and its cofactor NS2B constitute the protease. To function as an active enzyme, the NS3 protease requires the NS2B cofactor. NS2B is an integral membrane protein of 14 kDa that contains three domains: two trans-membrane segments located at the N- and C-termini and a central region of 47 amino acids (spanning amino-acids 49-96) that acts as an essential protein cofactor of the NS3 protease
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NS2B cofactor
protein essential for activity
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NS2B cofactor
protein required for proteolytic activity
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NS2B cofactor
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The dengue virus NS2B-NS3 protease (NS2B-NS3p), an important antiviral target for drug development, has been reported to adopt an open or closed conformation in crystal structures with different NS2B C-terminus (NS2Bc) positioning. NS2Bc departs from NS3p in the open conformation, but it forms tight interactions with NS3p in the closed conformation
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NS2B cofactor
the NS2B cofactor is covalently linked to the NS3 protease domain via a Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly (G4SG4) linker
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NS2B cofactor
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the unique cofactor region of zika virus NS2B-NS3 protease facilitates cleavage of key host proteins. The NS2B cofactor region from Zika virus protease is essential for recognition of host cell substrates. Replacing the NS2B region in another flavivirus protease enabled recognition of novel Zika-specific substrates by hybrid proteases, suggesting that the cofactor is the principal determinant in ZVP substrate selection
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NS2B cofactor
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the viral protease is a two-component serine protease formed by the N-terminal part of NS3 and the cofactor region of NS2B, a membrane protein essential for the membrane location of NS3. The NS2B mutant C143S cannot form the disulfide bond between C143 residues of neighboring NS3
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NS2B protein
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NS2B protein
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all cleavages of the flavivirus polyprotein mediated by the NS3 protease are believed to require cofactor activity of the NS2B protein
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NS2B protein
the G(22) residue and the negatively charged patch D(32)DD(34) of NS2B are part of an important configuration required for NS2B-NS3pro activity
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NS2B protein
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tight complementarity of the protein sequences is necessary for NS2B-dependent activation of NS3. NS2B residues V88 and Q77 are critical for Alkhurma virus NS3 activation. Poor conservation of the NS2B cofactor sequence within members of the genus Flavivirus
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additional information
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characterization of structural determinants necessary for activation of the enzyme by the NS2B cofactor. Alanine substitutions at residues Trp62, Leu75, and Ile79 in the NS2B cofactor result in marked effects on autoprocessing at the NS2B/NS3 site. The activity of the mutant NS3 protease with the synthetic peptide substrate Gly-Arg-Arg-4-methylcoumarin 7-amide is mainly affected by significantly reduced kcat values
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additional information
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chimeric proteases with the NS2B cofactor from Langat virus and Dengue virus type 3 replacing the Alkhurma virus sequence activate NS3pro
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