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Literature summary for 3.4.21.91 extracted from

  • Gouvea, I.E.; Izidoro, M.A.; Judice, W.A.; Cezari, M.H.; Caliendo, G.; Santagada, V.; dos Santos, C.N.; Queiroz, M.H.; Juliano, M.A.; Young, P.R.; Fairlie, D.P.; Juliano, L.
    Substrate specificity of recombinant dengue 2 virus NS2B-NS3 protease: influence of natural and unnatural basic amino acids on hydrolysis of synthetic fluorescent substrates (2007), Arch. Biochem. Biophys., 457, 187-196.
    View publication on PubMed
Search for more literature for 3.4.21.91

Cloned(Commentary)

Cloned (Comment) Organism
CF40glyNS3pro chimeric protease tagged with six consecutive histidines expressed in Escherichia coli strain SG13009 Dengue virus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 strongly inhibits Dengue virus
Na2SO4 kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl- Dengue virus
NaCl kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl- Dengue virus
NaOAc kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl- Dengue virus
Sodium citrate kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl- Dengue virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0039
-
 o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.004
-
 o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0045
-
 o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0046
-
 o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0059
-
 o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0069
-
 o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0072
-
 o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0074
-
 o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0088
-
 o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.0098
-
 o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0138
-
 o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0138
-
 o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.0165
-
 o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0177
-
 o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.0183
-
 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.02
-
 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0213
-
 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0237
-
 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0256
-
 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.026
-
 N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.026
-
 o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.027
-
 N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.027
-
 o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.03
-
 o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.0314
-
 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.038
-
 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.043
-
 N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.0532
-
 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.058
-
 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0588
-
 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0673
-
 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.0692
-
 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.216
-
 N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.247
-
 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.247
-
 Bz-Arg-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.298
-
 N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.354
-
 N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.49
-
 N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus

Organism

Organism UniProt Comment Textmining
Dengue virus
-
 type 2, strain NGC and strain BR/01-EN
-

Purification (Commentary)

Purification (Comment) Organism
on Ni2+ column Dengue virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bz-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus Bz-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information o-aminobenzoyl-EPEKQRTPQDNQQ-N-(2,4-dinitrophenyl)-ethylenediamine, o-aminobenzoyl-AGRRPAQ-N-(2,4-dinitrophenyl)-ethylenediamine and o-aminobenzoyl-AKRRRPQ-N-(2,4-dinitrophenyl)-ethylenediamine are not hydrolyzed Dengue virus ?
-
 ?
N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-3-pyridyl-L-Ala-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzoyl-4-(guanidino)-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
-
 Dengue virus N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AGKK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AGKR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AGRK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AGRR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AKKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AKKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-AKRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O is most efficiently cleaved. Substrates containing two or three basic amino acids (Arg or Lys) in tandem are best Dengue virus o-aminobenzoyl-AKRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-ARKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-ARKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-ARRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-ARRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-EVKKQR + AGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-FAAGRK + SLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O is poorly hydrolyzed Dengue virus o-aminobenzoyl-HRREKR + SVALQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-LNRRRR + TAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-RTSKKR + SWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus ?
-
 ?
o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Dengue virus o-aminobenzoyl-TTSTRR + GTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?

Synonyms

Synonyms Comment Organism
NS2B-NS3 protease
-
 Dengue virus
NS3 protease
-
 Dengue virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.013
-
 N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.013
-
 N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.014
-
 N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.02
-
 o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.02
-
 o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.022
-
 N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.03
-
 o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.035
-
 N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.04
-
 o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.04
-
 o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.04
-
 o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.04
-
 o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.04
-
 o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.04
-
 o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.05
-
 o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.07
-
 o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.07
-
 o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.09
-
 o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.09
-
 o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.109
-
 Bz-Arg-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.11
-
 N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.11
-
 o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.11
-
 o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.13
-
 o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.14
-
 o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.14
-
 o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.16
-
 o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.17
-
 o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.172
-
 N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
 Dengue virus
0.18
-
 o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.2
-
 o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.21
-
 o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.29
-
 o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.29
-
 o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine NS3 protease from strain NGC Dengue virus
0.34
-
 o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
0.38
-
 o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus
1
-
 o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
 Dengue virus

Cofactor

Cofactor Comment Organism Structure
NS2B protein
-
 Dengue virus