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Literature summary for 3.4.21.91 extracted from
- Functional characterization of cis and trans activity of the Flavivirus NS2B-NS3 protease (2007), J. Biol. Chem., 282, 12883-12892.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | formation of an active protease does not require neither cleavage of NS2B from NS3 nor a free NS3 N-terminus | West Nile virus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| plasmid pDV2-NS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2 | Dengue virus type 2 |
| plasmid pDV2-NS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2 | Dengue virus type 4 |
| plasmid pJEVNS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2 | Japanese encephalitis virus |
| plasmid pWNV-NS2B79-NS3FL expressed in Escherichia coli strain Rosetta2 | West Nile virus |
| plasmid pYFV-NS2B40-G4SG4-HM-NS3FL expressed in Escherichia coli strain Rosetta2 | Yellow fever virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G460L | no autolytic cleavage, but is an efficient enzyme against the artificial substrate CFP-LQYTKRGGVLWD-YFP | West Nile virus |
| S135A | no autolytic cleavage, is enzymatically inactive | West Nile virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | product inhibition of the protease by the cleaved C-terminus of NS2B. The reaction product Lys-Arg-COO- remains in the P2-P1 site after cleavage and effectively blocks the active site for both cis and trans substrates | West Nile virus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dengue virus type 2 | - |
- |
- |
| Dengue virus type 4 | - |
- |
- |
| Japanese encephalitis virus | - |
- |
- |
| West Nile virus | - |
- |
- |
| West Nile virus NY99 | - |
- |
- |
| Yellow fever virus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by anion-exchange chromatography | Yellow fever virus |
| by anion-exchange chromatography | West Nile virus |
| by anion-exchange chromatography | Dengue virus type 2 |
| by anion-exchange chromatography | Dengue virus type 4 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 15% glycerol | West Nile virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CFP-LQYTKRGGVLWD-YFP + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus | CFP-LQYTKR + GGVLWD-YFP | - |
? |  |
| CFP-LQYTKRGGVLWD-YFP + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus NY99 | CFP-LQYTKR + GGVLWD-YFP | - |
? | |
| CFP-SAAQRRGRIGRN-YFP + H2O | cleavage is barely detectable | West Nile virus | CFP-SAAQRR + GRIGRN-YFP | - |
? | |
| CFP-SAAQRRGRIGRN-YFP + H2O | cleavage is barely detectable | West Nile virus NY99 | CFP-SAAQRR + GRIGRN-YFP | - |
? | |
| LQYTKRGGVLWD + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus | LQYTKR + GGVLWD | - |
? | |
| LQYTKRGGVLWD + H2O | the artificial substrate contains the natural sequence of the NS2B/NS3 junction and is cleaved efficiently | West Nile virus NY99 | LQYTKR + GGVLWD | - |
? | |
| additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Yellow fever virus | ? | - |
? | |
| additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Japanese encephalitis virus | ? | - |
? | |
| additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Dengue virus type 2 | ? | - |
? | |
| additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage | Dengue virus type 4 | ? | - |
? | |
| additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage. It catalyzes two autolytic cleavages. The NS2B/NS3 junction is cleaved before protein purification. A second site at Arg4592Gly460 within the C-terminal helicase region of NS3 is cleaved more slowly. Intramolecular cleavage of both the N-terminal site and the internal helicase site of recombinant NS2B-NS3. The artificial substrates CFP-LQYTHMGGVLWD-YFP and CFP-GGGGHMGGVLWD-YFP are not cleaved | West Nile virus | ? | - |
? | |
| additional information | the recombinant NS2B-NS3 protease is subject to autolytic cleavage. It catalyzes two autolytic cleavages. The NS2B/NS3 junction is cleaved before protein purification. A second site at Arg4592Gly460 within the C-terminal helicase region of NS3 is cleaved more slowly. Intramolecular cleavage of both the N-terminal site and the internal helicase site of recombinant NS2B-NS3. The artificial substrates CFP-LQYTHMGGVLWD-YFP and CFP-GGGGHMGGVLWD-YFP are not cleaved | West Nile virus NY99 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NS2B-NS3 protease | - |
Yellow fever virus |
| NS2B-NS3 protease | - |
West Nile virus |
| NS2B-NS3 protease | - |
Japanese encephalitis virus |
| NS2B-NS3 protease | - |
Dengue virus type 2 |
| NS2B-NS3 protease | - |
Dengue virus type 4 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NS2B protein | - |
Yellow fever virus | |
| NS2B protein | - |
West Nile virus | |
| NS2B protein | - |
Japanese encephalitis virus | |
| NS2B protein | - |
Dengue virus type 2 | |
| NS2B protein | - |
Dengue virus type 4 |
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