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3.4.21.91: Flavivirin

This is an abbreviated version!
For detailed information about Flavivirin, go to the full flat file.

Word Map on EC 3.4.21.91

Reaction

Selective hydrolysis of -Xaa-Xaa-/-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala =

Synonyms

Dengue NS2b-NS3 protease, Dengue NS3 protease, dengue virus non-structural protein 3, dengue virus NS2B-NS3 protease, dengue virus protease, dengue virus serotype 2 protease, DENV NS2B-NS3, DENV-2 NS2B-NS3 protease, DENV2 NS2BNS3pro, DV2 NS2B/NS3pro, flavivirus NS2B-NS3 protease, non-structural protein 3 serine protease, non-structural protein NS2B-NS3 protease, nonstructural protein 3, NS2-3 protease, NS2B-3 proteinase, NS2B-NS3 complex, NS2B-NS3 protease, NS2B-NS3 protease-helicase, NS2B-NS3 proteinase, NS2B-NS3 proteolytic complex, NS2B-NS3 serine protease, NS2B-NS3 serine proteinase, NS2B-NS3(pro)teinase, NS2b-NS3-protease, NS2B-NS3p, NS2B-NS3pro, NS2B/3 protease, NS2B/NS3, NS2B/NS3 complex, NS2B/NS3 protease, NS2B/NS3 protease complex, NS2B/NS3 proteinase, NS2B/NS3 serine protease, NS2B/NS3pro serine protease, NS2B18NS3, NS2BNS3(pro)tease, NS3, NS3 protease, NS3 proteinase, NS3 serine protease, NS3pro, two-component NS2B-NS3 protease, viral serine protease NS2B/NS3, WNV NS2B-NS3, Yellow fever virus (flavivirus) protease, yellow fever virus NS2B-NS3-181 protease, YFV NS2B/NS3 protease, Zika protease, Zika virus NS2B-NS3 protease, ZIKV NS2B/NS3 protease, ZIKV protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.91 Flavivirin

Cloned

Cloned on EC 3.4.21.91 - Flavivirin

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CF40glyNS3pro chimeric protease tagged with six consecutive histidines expressed in Escherichia coli strain SG13009
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cloning of the NS3 protease domain (NS3 S1-E171), the active deltaNS2B–NS3pro complex (NS3pro domain fused with NS2B M48-A94 and L126-R131 regions), the deltaNS2B-NS3pro HDA complex (with S138A), mutated proteins and chimeric proteases with the NS2B cofactor from Langat virus and Dengue virus type 3 replacing the Alkhurma virus sequence. PCR products cloned into pET-DEST42 vector
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codon optimized cDNA encoding NS2B-NS3, recombinant expression of His-tagged wild-type enzyme and constructs in Escherichia coli strain BL21(DE3)
complete NS3 molecule fused to 18 residues of the NS2B cofactor or NS3 helicase (residues 177 to 618) cloned into pET32b vector and expressed in Escherichia coli BL21-CodonPlus
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deltaNS2B-NS3pro and deltaNS2B-NS3proHDA expressed as a hexahistidine-tagged protein in Escherichia coli BL21-CodonPlus(DE3)-RIL cells
Dengue virus DEN4 polyprotein
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expession in Pichia pastoris
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expressed in Escherichia coli
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expressed in Escherichia coli CodonPlus (DE3)-RIPL cells
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expressed in HEK293 cells
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expressed in Human hepatocellular carcinoma cells (Huh7 cells)
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expression in Escherichia coli
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expression in Escherichia coli BL21(DE3)
expression of NS2B(H)-NS3pro complex in E.scherichiacoli
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into the pET101 and expressed in Escherichia coli BL21(DE3) Codon Plus cells
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mutant K48A cloned into pET101/D-TOPO vector and expressed in Escherichia coli BL21(DE3) Codon Plus cells
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NS2B(H)-NS3pro expressed in Escherichia coli
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NS2B(H)–NS3pro expressed in Escherichia coli
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NS2B-NS3 proteolytic complex
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NS2B-NS3pro construct cloned into the pET101/D-TOPO vector C-terminally tagged with the His x 6 tag and expressed in Escherichia coli BL21(DE3) Codon Plus cells
NS2B-NS3pro wild-type and K48A constructs cloned into pET101 vector and expressed in Escherichia coli BL21 (DE3) Codon Plus cells
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NS2pro (NS2 residues 94-217), a truncated form of NS2 shown to be proteolytically active in the context of an NS2-3 precursor that includes the NS3 protease domain, expressed in Escherichia coli. NS2 containing wild-type or mutant active site residues expressed in mammalian cells in the context of a full-length viral polyprotein or as NS2-3 precursor with an N-terminal Flag or HA tag
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plasmid pDV2-NS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2
plasmid pJEVNS2B40-G4SG4-ASR-NS3FL expressed in Escherichia coli strain Rosetta2
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plasmid pWNV-NS2B79-NS3FL expressed in Escherichia coli strain Rosetta2
-
plasmid pYFV-NS2B40-G4SG4-HM-NS3FL expressed in Escherichia coli strain Rosetta2
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protocol for the preparation of the full-length NS2B/NS3 complex from dengue serotype 2 virus by utilizing a Mistic-fusion expression cassette in Escherichia coli
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recombinant expression of an active NS3pro domain fused with the NS2B cofactor (NS2BLNNS3pro) in a prokaryotic expression system
recombinant expression of codon-optimized N-terminally His6-tagged dengue virus serotype 2 protease (DENV2 NS2B-NS3pro)from linked and unlinked constructs in Escherichia coli strain BL21(DE3)
recombinant expression of His-tagged enzyme complex mutant H51A/S75C in Escherichia coli strain BL21(DE3)
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recombinant overexpression of His6-SUMO-tagged linked and unlinked NS3-NS2B(45-95) complex linked via G4SG4 linker in Escherichia coli strain BL21 Codon Plus (DE3)
separate recombinant expression of N-terminally His-tagged DENV virus 2 NS2B and NS3pro in Escherichia coli in inclusion bodies
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single-chain complex of NS2B/NS3 protease (CF40-gly-NS3pro190) expressed as an N-terminally His-tagged fusion protein in Escherichia coli
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the dengue 2 NS2B-NS3pro construct contains 255 residues, including a (Gly)4-Ser-(Gly)4 linker between NS2B (47 residues) and NS3pro (185 residues). In addition, it contains a His-tag at the C-terminus and the T7 gene 10 N-terminal peptide MASMTG at the N-terminus followed by a two-residue cloning artifact (LE), resulting in a 27 kDa protein. To suppress slow autocleavage detected by SDS-PAGE, most samples have the mutation Lys15Ala which cause no significant changes in the appearance of the NMR spectra. Protein is expressed in Escherichia coli
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using recombinant vaccinia virus expression system
wild-type Dengue virus and West Nile virus NS2B-NS3pro, together with West Nile virus/Dengue virus chimeras expressed with C-terminal His tags in Escherichia coli BL21(DE3) Codon Plus cells
Yellow fever virus polyprotein
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Yellow fever virus polyprotein (NS2B protein and NS3 proteinase domain)
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