Literature summary for 3.4.21.91 extracted from

Phoo, W.W.; Li, Y.; Zhang, Z.; Lee, M.Y.; Loh, Y.R.; Tan, Y.B.; Ng, E.Y.; Lescar, J.; Kang, C.; Luo, D.
Structure of the NS2B-NS3 protease from Zika virus after self-cleavage (2016), Nat. Commun., 7, 13410 .

Search for more literature for 3.4.21.91

Cloned(Commentary)

Cloned (Comment)	Organism
codon optimized cDNA encoding NS2B-NS3, recombinant expression of His-tagged wild-type enzyme and constructs in Escherichia coli strain BL21(DE3)	Zika virus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified NS2B-NS3 protease complexed with the last four amino acids of the NS2B cofactor bound at the NS3 active site, sitting drop vapour diffusion method, mixing of 0.001 ml of 40 mg/ml protein solution with 0.001 ml of reservoir solution containing 0.2 M Na-malonate, pH 4.0, 10% PEG 3350, 18°C, two days, X-ray diffraction structure determination and analysis at 1.84 A resolution	Zika virus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of eZiPro that contains the NS2B cofactor region (residues 45-96) linked with NS3 protein (residues 1-177) through the last five residues of NS2B (K126TGKR130), and the gZiPro construct, which is similar except that the G4SG4 linker replaced K126TGKR130 of NS2B. Protease activity analysis of gZiPro, eZiPro and bZiPro	Zika virus

Inhibitors

Inhibitors	Comment	Organism	Structure
Bovine pancreatic trypsin inhibitor	BPTI	Zika virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Zika virus

Organism

Organism	UniProt	Comment	Textmining
Zika virus	H8XX12	ZIKV	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	structure of the NS2B-NS3 protease from Zika virus after self-cleavage	Zika virus

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage via thrombin, dialysis, and gel filtration	Zika virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzoyl-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O	-	Zika virus	benzoyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin	-	?
additional information	the enzyme performs self-cleavage processes viral polyproteins. Protease activity analysis of gZiPro, eZiPro and bZiPro	Zika virus	?	-	?

Synonyms

Synonyms	Comment	Organism
non-structural protein NS2B-NS3 protease	-	Zika virus
NS2B-NS3 protease	-	Zika virus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Zika virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Zika virus

Cofactor

Cofactor	Comment	Organism	Structure
NS2B cofactor	-	Zika virus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	inhibition kinetics	Zika virus

General Information

General Information	Comment	Organism
additional information	structure of the NS2B-NS3 protease from Zika virus after self-cleavage. Analysis of the post-proteolysis state structure of the enzyme during viral polyprotein processing providing insights into peptide substrate recognition by the protease, Protease activity analysis of gZiPro, eZiPro and bZiPro	Zika virus
physiological function	the viral protease that processes viral polyproteins	Zika virus

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Release 2023.1 (January 2023)