Information on EC 3.4.21.91 - Flavivirin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.91
-
RECOMMENDED NAME
GeneOntology No.
Flavivirin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Selective hydrolysis of -Xaa-Xaa-/-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
serine endopeptidase
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CAS REGISTRY NUMBER
COMMENTARY hide
154215-26-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
polyprotein; strain D1/H/IMTSSA/98/606
SwissProt
Manually annotated by BRENDA team
polyprotein; strain D1/H/IMTSSA/98/606
SwissProt
Manually annotated by BRENDA team
strain 16681
-
-
Manually annotated by BRENDA team
strain Sri Lanka/1266/2000
SwissProt
Manually annotated by BRENDA team
strain Sri Lanka/1266/2000
SwissProt
Manually annotated by BRENDA team
strain TP-21
SwissProt
Manually annotated by BRENDA team
strain TP-21
SwissProt
Manually annotated by BRENDA team
strain MSI-7
-
-
Manually annotated by BRENDA team
strain MSI-7
-
-
Manually annotated by BRENDA team
strain Asibi
-
-
Manually annotated by BRENDA team
strain Ar-D-41644
-
-
Manually annotated by BRENDA team
strain Ar-D-41644
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
required for viral replication
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-AAGRK-SLTLnY-amide + H2O
Abz-AAGRK + SLTLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-AAGRKSLTL-3-nitrotyrosine-amide + H2O
Abz-AAGRK + SLTL-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-AGRK-SLTnY-amide + H2O
Abz-AGRK + SLTnY-amide
show the reaction diagram
-
-
-
-
?
Abz-AGRK-SnY-amide + H2O
Abz-AGRK + Ser-(3-nitro)Tyr-amide
show the reaction diagram
-
-
-
-
?
Abz-AGRKGTGN-3-nitrotyrosine-amide + H2O
Abz-AGRKGTGN + 3-nitrotyrosine-amide
show the reaction diagram
-
the poorest substrate
-
-
?
Abz-AGRKGTGNnY-amide + H2O
Abz-AGRK + GTGNnY-amide
show the reaction diagram
-
-
-
-
?
Abz-AGRKS-3-nitrotyrosine-amide + H2O
Abz-AGRKS + 3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-AGRKSLT-3-nitrotyrosine-amide + H2O
Abz-AGRK + SLT-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-ARRK-SLTLnY-amide + H2O
Abz-ARRK + SLTLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-ARRKSLTL-3-nitrotyrosine-amide + H2O
Abz-ARRK + SLTL-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-FAAGRK-SLTLnY-amide + H2O
Abz-FAAGRK + SLTLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-FAAGRKSLTL-3-nitrotyrosine-amide + H2O
Abz-FAAGRK + SLTL-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-GRK-SLTLnY-amide + H2O
Abz-GRK + SLTLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-GRK-SnY-amide + H2O
Abz-GRK + Ser-(3-nitro)Tyr-amide
show the reaction diagram
-
-
-
-
?
Abz-GRKS-3-nitrotyrosine-amide + H2O
Abz-GRK + S-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-GRKSLTL-3-nitrotyrosine-amide + H2O
Abz-GRK + SLTL-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-KKQR-AGVLnY-amide + H2O
Abz-KKQR + AGVLnY-amide
show the reaction diagram
-
high degree of cleavage efficiency, based on the NS2B/NS3 polyprotein cleavage site
-
-
?
Abz-KKQR-SAGMnY-amide + H2O
Abz-KKQR + SAGMnY-amide
show the reaction diagram
-
-
-
-
?
Abz-KKQRAGVL-3-nitrotyrosine-amide + H2O
Abz-KKQR + AGVL-3-nitrotyrosine-amide
show the reaction diagram
-
is based on the NS2B/NS3 polyprotein cleavage site, shows high degree of cleavage efficiency
-
-
?
Abz-KKQRSAGM-3-nitrotyrosine-amide + H2O
Abz-KKQR + SAGM-3-nitrotyrosine-amide
show the reaction diagram
-
substrates containing the capsid protein prime side sequence SAGM are efficiently hydrolysed
-
-
?
Abz-NRRRR-SAGMnYamide + H2O
?
show the reaction diagram
-
-
-
-
?
Abz-NRRRRSAGM-3-nitrotyrosine-amide + H2O
Abz-NRRRR + SAGM-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-RK-SLTLnY-amide + H2O
Abz-RK + SLTLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-RK-SnY-amide + H2O
Abz-RK + SnY-amide
show the reaction diagram
-
-
-
-
?
Abz-RKS-3-nitrotyrosine-amide + H2O
Abz-RK + S-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-RKSLTL-3-nitrotyrosine-amide + H2O
Abz-RK + SLTL-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-RRRR-3-nitrotyrosine-amide + H2O
Abz-RRRR + 3-nitrotyrosine-amide
show the reaction diagram
-
marginal activity
-
-
?
Abz-RRRR-nY-amide + H2O
Abz-RRRR + 3-nitro-L-tyrosine amide
show the reaction diagram
-
-
-
-
?
Abz-RRRR-SAGMnY-amide + H2O
Abz-RRRR + SAGMnY-amide
show the reaction diagram
-
-
-
-
?
Abz-RRRR-SAGnY-amide + H2O
Abz-RRRR + SAGnY-amide
show the reaction diagram
-
efficient substrate of the NS3 protease, based on the Dengue virus capsid protein processing site
-
-
?
Abz-RRRR-SLTLnY-amide + H2O
Abz-RRRR + SLTLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-RRRR-SnY-amide + H2O
Abz-RRRR + Ser-(3-nitro)Tyr-amide
show the reaction diagram
-
-
-
-
?
Abz-RRRRS-3-nitrotyrosine-amide + H2O
Abz-RRRR + S-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-RRRRSAG-3-nitrotyrosine-amide + H2O
Abz-RRRR + SAG-3-nitrotyrosine-amide
show the reaction diagram
-
most efficiently hydrolysed substrate, which contains the tetrabasic non-prime side sequence RRRR of the dengue virus capsid protein
-
-
?
Abz-RRRRSAGM-3-nitrotyrosine-amide + H2O
Abz-RRRR + SAGM-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-RRRRSLTL-3-nitrotyrosineamide + H2O
Abz-RRRR + SLTL-3-nitrotyrosineamide
show the reaction diagram
-
-
-
-
?
Abz-SKKR-AGVLnY-amide + H2O
Abz-SKKR + AGVLnY-amide
show the reaction diagram
-
-
-
-
?
Abz-SKKR-SAGMnY-amide + H2O
Abz-SKKR + SAGMnY-amide
show the reaction diagram
-
-
-
-
?
Abz-SKKRAGVL-3-nitrotyrosine-amide + H2O
Abz-SKKR + AGVL-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Abz-SKKRSAGM-3-nitrotyrosine-amide + H2O
Abz-SKKR + SAGM-3-nitrotyrosine-amide
show the reaction diagram
-
-
-
-
?
Ac-Thr-Arg-Arg-7-amido-4-methylcoumarin + H2O
Ac-Thr-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide + H2O
acetyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
ADP ATOO-647N + H2O
?
show the reaction diagram
-
affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase
-
-
?
AQRRGRIG + H2O
?
show the reaction diagram
-
three individual batches of the peptide results in 45%, 37%, and 36% cleavages
-
-
?
AQRRGRIG + H2O
AQRR + GRIG
show the reaction diagram
-
three individual batches of the peptide results in AQRR2GRIG 3%, 2%, and 0% cleavages
-
-
?
ATP ATOO-647N + H2O
?
show the reaction diagram
-
affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase
-
-
?
benzoyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide + H2O
benzoyl-Nle-Lys-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
benzoyl-Nle-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O
benzoyl-Nle-Lys-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-Nle-Lys-Arg-Arg-thiobenzylester + H2O
benzoyl-Nle-Lys-Arg-Arg + thiobenzoic acid
show the reaction diagram
-
-
-
-
?
benzoyl-Nle-Lys-Thr-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O
benzoyl-Nle-Lys-Thr-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-Nle-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O
benzoyl-Nle-Thr-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-Thr-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O
benzoyl-Thr-Lys-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzoyl-Thr-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin + H2O
benzoyl-Thr-Thr-Arg-Arg + 7-amino-3-carbamoylmethyl-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
benzyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
benzyloxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
substrate of isoform DENV-2
-
-
?
Boc-Gly-Arg-Arg-4-methylcoumaryl-7-amide + H2O
Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Boc-Gly-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
butyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
Bz-Arg-Arg-4-methylcoumaryl-7-amide + H2O
Bz-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
10fold higher affinity for the tetrapeptide than for the tripeptide Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin, and processes it with 34fold greater efficiency
-
-
?
CFP-LQYTKRGGVLWD-YFP + H2O
CFP-LQYTKR + GGVLWD-YFP
show the reaction diagram
CFP-SAAQRRGRIGRN-YFP + H2O
CFP-SAAQRR + GRIGRN-YFP
show the reaction diagram
Dengue virus DEN4 polyprotein + H2O
?
show the reaction diagram
-
involved in processing of downstream nonstructural proteins of the Dengue virus DEN4 polyprotein precursor
-
-
-
Dengue virus polyprotein + H2O
Dengue virus proteins NS3 and NS5
show the reaction diagram
DSSTKRGGSW + H2O
DSSTKR + GGSW
show the reaction diagram
-
each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed)
-
-
?
FAEGRRGAAEVLQ + H2O
FAEGRR + GAAEVLQ
show the reaction diagram
-
-
-
-
?
flavivirus polyprotein + H2O
?
show the reaction diagram
-
-
-
?
Flavivirus sp. polyproteins + H2O
?
show the reaction diagram
GLKRFGAK + H2O
GLKR + FGAK
show the reaction diagram
-
-
-
-
?
GLKRGSAK + H2O
GLKR + GSAK
show the reaction diagram
GLKRTGAK + H2O
GLKR + TGAK
show the reaction diagram
Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
KKQRAGVL + H2O
?
show the reaction diagram
-
cleaved with 11% efficiency
-
-
?
KLAQRRVFHGVAQ + H2O
KLAQRR + VFHGVAQ
show the reaction diagram
-
-
-
-
?
KPGLKRGGAK + H2O
KPGLKR + GGAK
show the reaction diagram
-
each substrate plasmid contains the ER membrane-anchoring domain, a specific cleavage sequence that includes the P6-P1 P19-P49 residues and a fluorescent reporter group (DsRed)
-
-
?
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
LQYTKRGGVLWD + H2O
LQYTKR + GGVLWD
show the reaction diagram
LYKRYGGF + H2O
?
show the reaction diagram
-
cleaved with 48% efficiency
-
-
?
MKTGRRGSANGKQ + H2O
MKTGRR + GSANGKQ
show the reaction diagram
-
-
-
-
?
MLKRGMPR + H2O
?
show the reaction diagram
-
cleaved with 49% efficiency
-
-
?
myelin basic protein + H2O
?
show the reaction diagram
-
-
-
?
N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O
N-alpha-benzoyl-L-arginine + p-nitroaniline
show the reaction diagram
N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-3-pyridyl-L-Ala-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O
N-benzoyl-4-(guanidino)-L-Phe-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide + H2O
N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
NQKRYGGF + H2O
?
show the reaction diagram
-
cleaved with 7% efficiency
-
-
?
NRKR + GGPA + H2O
NRKRGGPA
show the reaction diagram
-
two individual batches of the peptide results in 78 and 77% cleavages
-
-
?
NS2B-3181-polyproteins + H2O
?
show the reaction diagram
-
effect of mutations within Gly-Ala-Arg-Arg-+-Ser sequence on cis-cleavage (mutations at P1', P1, P2, P3 and P4), the term-+- depicts the point of cleavage
-
-
-
o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AGKK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AGKR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AGRK + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AGRR + SAQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AKKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AKKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AKRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-AKRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-ARKK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-ARKR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-ARRK + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-ARRR + SQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-EVKKQR + AGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-FAAGRK + SLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-HRREKR + SVALQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
is poorly hydrolyzed
-
-
?
o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KKR + SSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-KRR + SSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-LNRRRR + TAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-RTSKKR + SWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
?
show the reaction diagram
-
-
-
-
?
o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine + H2O
o-aminobenzoyl-TTSTRR + GTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine
show the reaction diagram
-
-
-
-
?
PhAc-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O
PhAc-Lys-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
substrate of isoform DENV-3
-
-
?
protein C of tick-borne encephalitis virus + H2O
?
show the reaction diagram
-
the protein contains two amino acid sequence motifs within the carboxy-terminal region that match the canonical NS2B/3 recognition site. The downstream site is necessary and sufficient for efficient cleavage and virion assembly. In contrast, the upstream site is dispensable and placed in a structural context that renders it largely inaccessible to the viral protease. The NS2B/3 protease predominantly uses the downstream cleavage motif R95-R96-S97 for cleavage
-
-
?
Pyr-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
Pyr-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Pyr-RTKR-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
pyroglutamic acid-Arg-Thr-Lys-Arg-4-methylcoumarin 7-amide + H2O
pyroglutamic acid-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin + H2O
pyroglutamic acid-Arg-Thr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
pyroglutamic acid-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O
pyroglutamic acid-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
QRRRGGTA + H2O
QRRR + GGTA
show the reaction diagram
-
two individual batches of the peptide results in 58% cleavage
-
-
?
RIFGRRSIPVNEQ + H2O
RIFGRR + SIPVNEQ
show the reaction diagram
-
-
-
-
?
SAAQRRGRIGRNQ + H2O
SAAQRRGRIGRNQ
show the reaction diagram
-
-
-
-
?
SSRKRRSHDVLTQ + H2O
SSRKRR + SHDVLTQ
show the reaction diagram
-
-
-
-
?
t-butoxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide + H2O
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide + H2O
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine + 7-amino-4-methylcoumarin
show the reaction diagram
tert-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
tert-butoxycarbonyl-Gly-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
VRGARRSGDVLWQ + H2O
VRGARR + SGDVLWQ
show the reaction diagram
-
-
-
-
?
Yellow fever virus protein + H2O
?
show the reaction diagram
YF17D virus protein + H2O
?
show the reaction diagram
-
cleavage site: Gln-Gln-Arg-+-Ser, i.e. 4A/2K site, this cleavage is prerequisite for processing at the downstream signalase site, determinants of cleavage site specificity for 2B/3 cleavage (site: Gly-Ala-Arg-Arg-+-Ser), the term-+- depicts the point of cleavage
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Dengue virus DEN4 polyprotein + H2O
?
show the reaction diagram
-
involved in processing of downstream nonstructural proteins of the Dengue virus DEN4 polyprotein precursor
-
-
-
flavivirus polyprotein + H2O
?
show the reaction diagram
-
-
-
?
Flavivirus sp. polyproteins + H2O
?
show the reaction diagram
-
involved in processing of downstream nonstructural proteins of the virus polyprotein
-
-
-
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NS2B protein
-
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
kcat/Km values decrease by adding NaCl up to 100 mM. This results from the systematic increase in Km values with the increase in NaCl concentration in the range 1 to 10 mM
Zn2+
-
auto-cleavage and NS3 protease activity are zinc-dependent. NS2/3 auto-cleavage activity is more sensitive to zinc chelation by 1,10-phenanthroline than the NS3 protease activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5-amino-1-phenylsulfonylpyrazol-3-yl) 2-bromobenzoate
-
100.8% inhibition at 0.05 mM
(5-amino-1-phenylsulfonylpyrazol-3-yl)thiophene-2-carboxylate
-
101.4% inhibition at 0.05 mM
(E)-N-(5-(benzylsulfanyl)-1,3,4-thiadiazol-2-yl)-2-cyano-3-(1-((2-cyanophenyl)methyl)-1H-indol-3-yl)prop-2-enamide
-
-
(E)-N-(5-(benzylsulfanyl)-1,3,4-thiadiazol-2-yl)-2-cyano-3-(1-((2-fluorophenyl)methyl)-1H-indol-3-yl)prop-2-enamide
-
-
(E)-N-(5-(benzylsulfanyl)-1,3,4-thiadiazol-2-yl)-2-cyano-3-(1-((4-fluorophenyl)methyl)-1H-indol-3-yl)prop-2-enamide
-
-
1,10-phenanthroline
-
zinc-selective chelator, inhibits NS2/3 auto-cleavage and NS3 protease activity
1-(2,6-difluorophenyl)-2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]ethanol
-
-
1-(2,6-difluorophenyl)-2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]ethanone
-
-
1-(2-fluorophenyl)-2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]ethanone
-
-
1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl 2,6-difluoro-3-methylbenzoate
-
-
1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl 2,6-difluorobenzoate
-
-
1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl benzoate
-
-
1-benzyl-3-methyl-1H-pyrazol-5-yl 2,6-difluoro-3-methylbenzoate
-
-
1-benzyl-3-methyl-1H-pyrazol-5-yl 2,6-difluorobenzoate
-
-
2-([[3,6-bis(2,6-dimethylphenyl)-7-oxo-2-thioxo-2,3,6,7-tetrahydro[1,3]thiazolo[4,5-d]pyrimidin-5-yl]sulfanyl]methyl)-7-chloro-4H-pyrido[1,2-a]pyrimidin-4-one
-
-
2-benzyl-N-[2-methyl-4-(4-methylpiperazin-1-yl)phenyl]-1,2,3,4-tetrahydrobenzo[b][1,6]naphthyridine-10-carboxamide
-
compound identified by a pharmacophore model based on X-ray crystal structure and predicted binding patterns. Compound shows good cell-based bioactivity
2-naphthoyl-Lys-Arg-Arg
2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]-1-phenylethanol
-
-
-
2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]-1-phenylethanone
-
-
2-[[(4-methoxyphenyl)amino]methylidene]cyclohexane-1,3-dione
-
56% inhibition at 0.05 mM
4-aminobenzoyl-Lys-Arg-Arg
4-aminobenzoyl-Lys-Lys-Arg
4-aminophenylacetyl-Lys-Arg-Arg
4-guanidino-benzoic acid 4-nitrophenyl ester
-
the fold of Dengue NS2B-NS3pro in solution with and without bound inhibitor by nuclear magnetic resonance spectroscopy is analyzed. Multiple paramagnetic lanthanide tags are attached to different sites to generate pseudocontact shifts (PCS). The PCSs show, that in the presence of a positively charged low-molecular weight inhibitor, the enzyme assumes a closed state that is very similar to the closed state previously observed for the West Nile virus protease. To assess the open state, a binding site for a Gd3+ complex is created and paramagnetic relaxation enhancements is measured. The results show that the specific open conformation displayed in the crystal of DEN NS2B-NS3pro is barely populated in solution
4-hydroxypanduratin
-
-
4-hydroxypanduratin A
-
cyclohexenyl chalcone derivative, competetive inhibitor, compound from Boesenbergia rotunda (L.) Mansf. Kulturpfl.
4-methylumbelliferyl-alpha-D-mannopyranoside
-
i.e. compound 4282211 of ZINC database, identified by molecular docking. Inhibitor can efficiently bind to the NS2b/NS3 protease without changing the conformation of the protein, most active inhibitor identified, acitve against serotypes DENV1, DENV2, DENV3
4-phenylphenylacetyl-Lys-Arg-Arg
4-phenylphenylacetyl-Lys-Lys-Arg
4-[3-acetyl-5-(2-phenylquinolin-4-yl)-2,3-dihydro-1,3,4-oxadiazol-2-yl]benzoic acid
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,3,4-trifluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,3,5,6-tetrafluoro-4-methylbenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,3,6-trifluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,4,5-trifluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,4,6-trifluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,4-difluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,5-difluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,6-dichlorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,6-difluoro-3-methylbenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,6-difluorobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2-ethoxybenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2-fluoro-3-(trifluoromethyl)benzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2-methylbenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2-nitrobenzoate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 3-chlorothiophene-2-carboxylate
-
-
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl thiophene-3-carboxylate
-
-
5-methyl-2-[4-[(4-[[4-(4-methyl-4,5-dihydro-1H-imidazol-3-ium-2-yl)phenyl]amino]-4a,8a-dihydrophthalazin-1-yl)amino]phenyl]-4,5-dihydro-1H-imidazol-3-ium
-
-
5-[(4-chloro-3-nitro-1H-pyrazol-1-yl)methyl]-N-[1-[2-(diethylamino)ethyl]-1H-benzimidazol-2-yl]furan-2-carboxamide
-
-
5-[(E)-2-(2,6-difluorophenyl)ethenyl]-1-(4-methoxybenzyl)-3-methyl-1H-pyrazole
-
-
6-methoxy-3-methyl-1H-benzo[de]cinnoline
-
98.2% inhibition at 0.05 mM
Ac-EVKKQR-NH2
-
-
Ac-FAAGRK-NH2
-
-
Ac-GKR-NH2
-
-
Ac-KKR-NH2
-
-
Ac-KR-NH2
-
-
Ac-RTSKKR-NH2
-
-
Ac-SKKR-NH2
-
-
Ac-TTSTRR-NH2
-
-
acetyl-Ala-Arg-agmatine
acetyl-Ala-Lys-agmatine
acetyl-Arg-Lys-agmatine
acetyl-Lys-Arg-Arg
acetyl-Lys-Lys-agmatine
acetyl-Lys-Lys-Arg
acetyl-Trp-Lys-agmatine
agathisflavone
-
binds to a specific allosteric binding site of the enzyme
alpinetin
-
compound from Boesenbergia rotunda (L.) Mansf. Kulturpfl.
antibody D05320
-
-
-
antibody D05321
-
-
-
antibody D05322
-
-
-
antibody D05323
-
-
-
antibody D05444
-
-
-
antibody D05445
-
-
-
antibody D05446
-
-
-
Aprotinin
benzoyl-Lys-Lys(acetyl)-Arg
benzoyl-Lys-Lys(benzoyl)-Arg
benzoyl-Lys-Lys(benzyl)-Arg
benzoyl-Lys-Lys(p-anisoyl)-Arg
benzoyl-Lys-Lys-Arg
benzoyl-Nle-Lys-Arg-Arg
benzoyl-Nle-Lys-Thr-Arg-H
-
-
Bovine pancreatic trypsin inhibitor
-
pseudocontact shifts from a lanthanide tag show that NS2Bc remains in the closed conformation also in the complex with
-
Bowman-Birk inhibitor
-
from mung bean
Bz-Ala-Lys-Arg-Arg
-
-
Bz-Ala-Lys-Arg-Arg-H
-
-
Bz-Arg-Arg
-
-
Bz-Arg-Arg-H
-
-
Bz-D-Nle-Lys-Arg-Arg-H
-
-
Bz-Lys-Arg-Arg
-
-
Bz-Lys-Arg-Arg-H
-
-
Bz-N-Me-Nle-Lys-Arg-Arg-H
-
-
Bz-Nle-(3-amino)-Phe-(3-imino)-Phe-(3,4-dimethoxy)-Phe
-
-
Bz-Nle-(3-guanidino)-Phe-(4-guanidino)-Phe-(4-guanidino)-Phe
-
-
Bz-Nle-(4-amino)-Phe-(3-guanidino)-Phe-(4-amino)-Phe
-
-
Bz-Nle-(4-amino)-Phe-(3-guanidino)-Phe-Arg
-
-
Bz-Nle-(4-amino)-Phe-(3-imino)-Phe-Arg
-
-
Bz-Nle-(4-amino)-Phe-(4-guanidino)-Phe-Trp
-
-
Bz-Nle-Ala-Arg-Arg
-
-
Bz-Nle-Ala-Arg-Arg-H
-
-
Bz-Nle-Arg-(3-guanidino)-Phe-(4-amino)-Phe
-
-
Bz-Nle-D-Lys-Arg-Arg-H
-
-
Bz-Nle-His-(3-guanidino)-Phe-(4-guanidino)-Phe
-
-
Bz-Nle-His-(3-guanidino)-Phe-beta-(2-naphthyl)-Ala
-
-
Bz-Nle-His-(3-imino)-Phe-Arg
-
-
Bz-Nle-His-(4-guanidino)-Phe-(4-guanidino)-Phe
-
-
Bz-Nle-His-(4-guanidino)-Phe-Trp
-
-
Bz-Nle-homo-His-(3-guanidino)-Phe-(4-guanidino)-Phe
-
-
Bz-Nle-homoHis-(3-guanidino)-Phe-Arg
-
-
Bz-Nle-homoHis-(4-guanidino)-Phe-(4-phenyl)-Phe
-
-
Bz-Nle-Lys-Ala-Arg
-
-
Bz-Nle-Lys-Ala-Arg-H
-
-
Bz-Nle-Lys-Arg-(p-Cl)Phe-H
-
-
Bz-Nle-Lys-Arg-(p-CN)Phe-H
-
-
Bz-Nle-Lys-Arg-(p-guanidinyl)Phe-H
-
-
Bz-Nle-Lys-Arg-(p-Me)Phe-H
-
-
Bz-Nle-Lys-Arg-(p-Ph)Phe-H
-
-
Bz-Nle-Lys-Arg-Ala
-
-
Bz-Nle-Lys-Arg-Ala-H
-
-
Bz-Nle-Lys-Arg-Arg
-
-
Bz-Nle-Lys-Arg-Arg(OH)-CONH-Bn
-
-
Bz-Nle-Lys-Arg-Arg-B(OH)2
-
-
Bz-Nle-Lys-Arg-Arg-benzoxazole
-
-
Bz-Nle-Lys-Arg-Arg-CF3
-
-
Bz-Nle-Lys-Arg-Arg-H
-
-
Bz-Nle-Lys-Arg-Arg-NH2
-
-
Bz-Nle-Lys-Arg-Arg-NHSO2CF3
-
-
Bz-Nle-Lys-Arg-Arg-OH
-
-
Bz-Nle-Lys-Arg-Arg-thiazole
-
-
Bz-Nle-Lys-Arg-D-Arg-H
-
-
Bz-Nle-Lys-Arg-homoPhe-H
-
-
Bz-Nle-Lys-Arg-Lys
-
-
Bz-Nle-Lys-Arg-Lys-H
-
-
Bz-Nle-Lys-Arg-Phe
-
-
Bz-Nle-Lys-Arg-Phe-H
-
-
Bz-Nle-Lys-Arg-Phg-H
-
-
Bz-Nle-Lys-Arg-Trp
-
-
Bz-Nle-Lys-Arg-Trp-H
-
-
Bz-Nle-Lys-D-Arg-Arg-H
-
-
Bz-Nle-Lys-Lys-Arg
-
-
Bz-Nle-Lys-Lys-Arg-B(OH)2
-
-
Bz-Nle-Lys-Lys-Arg-H
-
-
Bz-Nle-Lys-N-Me-Arg-Arg-H
-
-
Bz-Nle-Lys-Phe-Arg
-
-
Bz-Nle-Lys-Phe-Arg-H
-
-
Bz-Nle-Lys-Pro-Arg-H
-
-
Bz-Nle-Lys-Thr-Arg-H
-
-
Bz-Nle-Lys-Thr-Arg-NH2
-
-
Bz-Nle-Lys-Thr-Arg-OH
-
-
Bz-Nle-N-Me-Lys-Arg-Arg-H
-
-
Bz-Nle-Phe-Arg-Arg
-
-
Bz-Nle-Phe-Arg-Arg-H
-
-
Bz-Nle-Pro-Arg-Arg-H
-
-
Bz-Phe-Lys-Arg-Arg
-
-
Bz-Phe-Lys-Arg-Arg-H
-
-
CaCl2
-
strongly inhibits
cardamonin
-
chalcone, non-competetive inhibitor, compound from Boesenbergia rotunda (L.) Mansf. Kulturpfl.
cinnamoyl-Lys-Arg-Arg
cyclopropionyl-Lys-Lys-Arg
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
-
completely inhibits autolysis of the NS2B-NS3pro construct
EDTA
-
zinc-selective chelator, inhibits NS2/3 auto-cleavage and NS3 protease activity
myricetin
-
binds to a specific allosteric binding site of the enzyme
N-(6-nitro-2,3-dihydro-1,3-benzothiazol-2-yl)-2-([4-(trifluoromethyl)phenyl]sulfanyl)benzamide
-
EC50 value 0.1 microM
N-(6-nitro-2,3-dihydro-1,3-benzothiazol-2-yl)-3-[(4-nitrophenyl)sulfanyl]benzamide
-
EC50 value 0.3 microM
N-(9-ethylcarbazol-3-yl)-2-[(4-ethyl-5-furan-2-yl-1,2,4-triazol-3-yl)sulfanyl]acetamide
-
68.8% inhibition at 0.05 mM
N-[(2S)-6-amino-1-([(2S)-6-amino-1-[(3R)-3-(carbamimidamidomethyl)pyrrolidin-1-yl]-1-oxohexan-2-yl]amino)-1-oxohexan-2-yl]-2-(biphenyl-4-yl)acetamide
-
-
N-[(2S)-6-amino-1-([(2S)-6-amino-1-[(3S)-3-(carbamimidamidomethyl)pyrrolidin-1-yl]-1-oxohexan-2-yl]amino)-1-oxohexan-2-yl]-2-(biphenyl-4-yl)acetamide
-
-
N-[(2S)-6-amino-1-([(2S)-6-amino-1-[4-(carbamimidamidomethyl)piperidin-1-yl]-1-oxohexan-2-yl]amino)-1-oxohexan-2-yl]-2-(biphenyl-4-yl)acetamide
-
-
N2-(biphenyl-3-ylacetyl)-L-lysyl-N-[(1-carbamimidoylpiperidin-4-yl)methyl]-L-lysinamide
-
C-terminal agmatine peptidomimetic inhibitor, competitive
N2-(biphenyl-4-ylacetyl)-L-lysyl-N-(4-carbamimidamidobutyl)-L-lysinamide
-
-
N2-(biphenyl-4-ylacetyl)-L-lysyl-N-(trans-4-carbamimidamidocyclohexyl)-L-lysinamide
-
-
N2-(biphenyl-4-ylacetyl)-L-lysyl-N-[(2E)-4-carbamimidamidobut-2-en-1-yl]-L-lysinamide
-
-
N2-(biphenyl-4-ylcarbonyl)-L-lysyl-N-(2-carbamimidamidoethyl)-L-lysinamide
-
-
N2-(biphenyl-4-ylcarbonyl)-L-lysyl-N-(3-carbamimidamidopropyl)-L-lysinamide
-
-
N2-(biphenyl-4-ylcarbonyl)-L-lysyl-N-(5-carbamimidamidopentyl)-L-lysinamide
-
-
N2-acetyl-L-lysyl-N-(4-carbamimidamidobutyl)-L-lysinamide
-
-
Na2SO4
-
kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl-
NaOAc
-
kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl-
nona-D-Arg-amide
-
completely inhibits autolysis of the NS2B-NS3pro construct
panduratin A
phenylacetyl-Lys-Arg-Arg
phenylacetyl-Lys-Lys-Arg
phenylpropionyl-Lys-Arg-Arg
pinocembrin
-
compound from Boesenbergia rotunda (L.) Mansf. Kulturpfl.
pinostrobin
-
flavanone, non-competetive inhibitor, compound from Boesenbergia rotunda (L.) Mansf. Kulturpfl.
propionyl-Lys-Arg-Arg
propionyl-Lys-Lys-Arg
quercetin
SID-3717586
-
-
SID-4245669
-
-
SID-852843
-
-
Sodium citrate
-
kosmotropic salts of the Hofmeister’s series strongly inhibit in decreasing order: sodium citrate, SO42-, acetate, Cl-
trifluoroacetyl-Lys-Lys-Arg
[2-(2-cyanoethyl)-5-methylpyrazol-3-yl] benzoate
-
105.9% inhibition at 0.05 mM
[4-(2,3-dimethylphenyl)piperazin-1-yl]-(2-ethylsulfonyl-3,4-dihydro-1H-isoquinolin-3-yl)methanone
-
51.6% inhibition at 0.05 mM
[4-methyl-5-(3-methylbut-2-en-1-yl)-6-phenylcyclohex-3-en-1-yl](2,4,6-trimethoxyphenyl)methanone
-
-
-
[5-(3-methylbut-2-en-1-yl)-6-phenylcyclohex-3-en-1-yl](2,4,6-trimethoxyphenyl)methanone
-
-
-
[5-amino-1-(4-fluorophenyl)sulfonylpyrazol-3-yl] furan-2-carboxylate
-
107.7% inhibition at 0.05 mM
[5-amino-1-(4-fluorophenyl)sulfonylpyrazol-3-yl] thiophene-2-carboxylate
-
99.1% inhibition at 0.05 mM
[5-amino-1-(4-methoxyphenyl)sulfonylpyrazol-3-yl] 2-bromobenzoate
-
112.5% inhibition at 0.05 mM
[5-amino-1-(4-methoxyphenyl)sulfonylpyrazol-3-yl] benzoate
-
115.6% inhibition at 0.05 mM
[5-amino-1-(4-methoxyphenyl)sulfonylpyrazol-3-yl] furan-2-carboxylate
-
99.3% inhibition at 0.05 mM
[5-amino-1-(4-methoxyphenyl)sulfonylpyrazol-3-yl] thiophene-2-carboxylate
-
116.3% inhibition at 0.05 mM
[5-amino-1-(4-methylphenyl)sulfonylpyrazol-3-yl] furan-2-carboxylate
-
96.6% inhibition at 0.05 mM
[5-amino-1-(4-methylphenyl)sulfonylpyrazol-3-yl] thiophene-2-carboxylate
-
86.8% inhibition at 0.05 mM
[6-(4-methoxyphenyl)-4-methyl-5-(3-methylbut-2-en-1-yl)cyclohex-3-en-1-yl](2,4,6-trimethoxyphenyl)methanone
-
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NS2B
-
viral activator protein
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.089
Abz-KKQRAGVL-3-nitrotyrosine-amide
-
-
0.0045
Abz-RRRRSAG-3-nitrotyrosine-amide
-
-
0.0045
Abz-RRRRSAGnY-amide
-
-
0.1
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide
-
pH 9.5, 37°C
0.0016 - 8.6
benzoyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide
0.0062 - 7.3
benzoyl-Nle-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
6
benzoyl-Nle-Lys-Arg-Arg-thiobenzylester
-
pH 8.5, 37°C, enzyme from Dengue virus serotype 4
0.034 - 52
benzoyl-Nle-Lys-Thr-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.046 - 72
benzoyl-Nle-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.0066 - 8.6
benzoyl-Thr-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.076 - 210
benzoyl-Thr-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.143 - 0.707
benzyloxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide
0.1422
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin
-
-
0.004 - 0.03
butyloxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin
0.247
Bz-Arg-Arg-4-methylcoumaryl-7-amide
-
-
0.0146
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin
-
-
0.015
FAEGRRGAAEVLQ
-
pH 9.0, 37°C
0.146
Gly-Arg-Arg-4-methylcoumarin 7-amide
-
pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor
0.0027
KLAQRRVFHGVAQ
-
pH 9.0, 37°C
0.0013 - 0.089
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin
0.0025
MKTGRRGSANGKQ
-
pH 9.0, 37°C
0.3 - 15.9
N-alpha-benzoyl-L-arginine-p-nitroanilide
0.027
N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
-
0.216
N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
-
0.298
N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
-
0.043
N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide
-
-
0.026
N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
-
0.354
N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
-
0.49
N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
-
-
0.247
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
-
0.0087 - 0.0588
o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0073 - 0.038
o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.005 - 0.0213
o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.02
o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0066 - 0.0692
o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0076 - 0.0532
o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0006 - 0.0237
o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.002 - 0.0314
o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.00013 - 0.0673
o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.014 - 0.058
o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0015 - 0.0183
o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0096 - 0.0256
o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.0088
o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
NS3 protease from strain NGC
0.0177
o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
NS3 protease from strain NGC
0.0074
o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.026
o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
NS3 protease from strain NGC
0.012
o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
pH 9.0, 37°C
0.0021
o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
pH 9.0, 37°C
0.0039
o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
NS3 protease from strain NGC
0.03
o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
NS3 protease from strain NGC
0.004
o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0098
o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0072
o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0046
o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0045
o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0138
o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
; NS3 protease from strain NGC
0.0069
o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0165
o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.0059
o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
-
0.027
o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine
-
NS3 protease from strain NGC
0.004 - 0.071
pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
0.02
RIFGRRSIPVNEQ
-
pH 9.0, 37°C
0.0088
SAAQRRGRIGRNQ
-
pH 9.0, 37°C
0.0022
SSRKRRSHDVLTQ
-
pH 9.0, 37°C
0.036 - 0.04
t-butoxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin
0.324 - 2.7
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
0.181 - 3.74
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
0.12 - 180
tert-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide
0.0085
VRGARRSGDVLWQ
-
pH 9.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
Abz-AGRK-SLTnY-amide
Dengue virus 2
-
-
0.001
Abz-AGRK-SnY-amide
Dengue virus 2
-
-
0.001
Abz-AGRKS-3-nitrotyrosine-amide
Dengue virus 2
-
-
0.005
Abz-AGRKSLT-3-nitrotyrosine-amide
Dengue virus 2
-
-
0.0967
Abz-KKQR-SAGMnY-amide
Dengue virus 2
-
-
0.0967
Abz-KKQRAGVL-3-nitrotyrosine-amide
Dengue virus 2
-
-
0.0045
Abz-SKKR-AGVLnY-amide
Dengue virus 2
-
-
0.045
Abz-SKKR-SAGMnY-amide
Dengue virus 2
-
-
0.0045
Abz-SKKRAGVL-3-nitrotyrosine-amide
Dengue virus 2
-
-
0.045
Abz-SKKRSAGM-3-nitrotyrosine-amide
Dengue virus 2
-
-
0.112
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide
Dengue virus
-
pH 9.5, 37°C
2.9
benzoyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide
Dengue virus
-
pH 8.5, 37°C, enzyme from Dengue virus serotype 4
0.32 - 2.8
benzoyl-Nle-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
300
benzoyl-Nle-Lys-Arg-Arg-thiobenzylester
Dengue virus
-
pH 8.5, 37°C, enzyme from Dengue virus serotype 4
0.97 - 4.9
benzoyl-Nle-Lys-Thr-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.28 - 1.1
benzoyl-Nle-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.05 - 0.43
benzoyl-Thr-Lys-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.17 - 0.48
benzoyl-Thr-Thr-Arg-Arg-7-amido-3-carbamoylmethyl-4-methylcoumarin
0.034
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin
Yellow fever virus
-
-
0.109
Bz-Arg-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.001
FAEGRRGAAEVLQ
Yellow fever virus
-
pH 9.0, 37°C
0.02
Gly-Arg-Arg-4-methylcoumarin 7-amide
Dengue virus 2
-
pH 9.0, 37°C, wild-type NS3 protease with wild-tyüe NS2B cofactor
0.003
KLAQRRVFHGVAQ
Yellow fever virus
-
pH 9.0, 37°C
0.013 - 7.3
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin
0.012
MKTGRRGSANGKQ
Yellow fever virus
-
pH 9.0, 37°C
0.0004 - 0.015
N-alpha-benzoyl-L-arginine-p-nitroanilide
0.013
N-benzoyl-3-(4-aminocyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.014
N-benzoyl-3-(4-piperidinyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.172
N-benzoyl-3-(trans-aminomethylcyclohexyl)-L-Ala-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.002
N-benzoyl-3-pyridyl-L-Ala-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.013
N-benzoyl-4-(aminomethyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.022
N-benzoyl-4-(aminomethyl-N-isopropyl)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.035
N-benzoyl-4-(guanidino)-L-Phe-L-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.11
N-benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
Dengue virus
-
-
0.001 - 0.02
o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.01 - 0.2
o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.005 - 0.13
o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.061 - 0.21
o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.004 - 0.04
o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.07 - 0.097
o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.012 - 0.04
o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.026 - 1
o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.002 - 0.05
o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.06 - 0.07
o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.005 - 0.09
o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.12 - 0.38
o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
0.03
o-aminobenzoyl-EVKKQRAGVLWDQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
NS3 protease from strain NGC
0.02
o-aminobenzoyl-FAAGRKSLTLNLQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
NS3 protease from strain NGC
0.14
o-aminobenzoyl-FAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.04
o-aminobenzoyl-HRREKRSVALQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
NS3 protease from strain NGC
0.012
o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
0.025
o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
0.04
o-aminobenzoyl-LNRRRRTAGMIIQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
NS3 protease from strain NGC
0.17
o-aminobenzoyl-RTSKKRSWPLNEQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
NS3 protease from strain NGC
0.09
o-aminobenzoyl-SAAGRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.04
o-aminobenzoyl-SAAQRKGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.11
o-aminobenzoyl-SAAQRRGLIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.11
o-aminobenzoyl-SAAQRRGRIGNNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.16
o-aminobenzoyl-SAAQRRGRIGRLQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.29
o-aminobenzoyl-SAAQRRGRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
; NS3 protease from strain NGC
0.18
o-aminobenzoyl-SAAQRRGRILRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.34
o-aminobenzoyl-SAAQRRGRTGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.14
o-aminobenzoyl-SAAQRRSRIGRNQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
-
0.04
o-aminobenzoyl-TTSTRRGTGNIGQ-N-(2,4-dinitrophenyl)-ethylenediamine
Dengue virus
-
NS3 protease from strain NGC
5.3 - 6.3
pyroglutamic acid-Arg-Thr-Lys-Arg-7-amido-4-methylcoumarin
0.005
RIFGRRSIPVNEQ
Yellow fever virus
-
pH 9.0, 37°C
0.016
SAAQRRGRIGRNQ
Yellow fever virus
-
pH 9.0, 37°C
0.069
SSRKRRSHDVLTQ
Yellow fever virus
-
pH 9.0, 37°C
0.18 - 0.22
t-butoxycarbonyl-Arg-Val-Arg-Arg-7-amido-4-methylcoumarin
0.007 - 0.163
t-butyloxycarbonyl-glycyl-L-arginyl-L-arginine-4-methylcoumaryl-7-amide
0.006 - 0.153
t-butyloxycarbonyl-glycyl-L-lysyl-L-arginine-4-methylcoumaryl-7-amide
0.03 - 0.13
tert-butoxycarbonyl-Gly-Arg-Arg-4-methylcoumarin 7-amide
0.041
VRGARRSGDVLWQ
Yellow fever virus
-
pH 9.0, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.12
acetyl-Nle-Lys-Arg-Arg-4-methylcoumarin 7-amide
Dengue virus
-
pH 9.5, 37°C
202430
0.07
FAEGRRGAAEVLQ
Yellow fever virus
-
pH 9.0, 37°C
41930
1.1
KLAQRRVFHGVAQ
Yellow fever virus
-
pH 9.0, 37°C
41931
82 - 100
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin
8874
4.8
MKTGRRGSANGKQ
Yellow fever virus
-
pH 9.0, 37°C
41932
0.11
o-aminobenzoyl-AGKKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18497
1.37
o-aminobenzoyl-AGKRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18499
1
o-aminobenzoyl-AGRKSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18498
3.04
o-aminobenzoyl-AGRRSAQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18496
0.6
o-aminobenzoyl-AKKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18505
12.8
o-aminobenzoyl-AKKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18507
20
o-aminobenzoyl-AKRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18506
13
o-aminobenzoyl-AKRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18501
18.16
o-aminobenzoyl-ARKKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18504
4.08
o-aminobenzoyl-ARKRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18502
3.33
o-aminobenzoyl-ARRKSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18503
12.5
o-aminobenzoyl-ARRRSQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
18500
1
o-aminobenzoyl-KKRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
41934
11.9
o-aminobenzoyl-KRRSSKQ-N-(2,4-dinitrophenyl)-ethylenediamine
Yellow fever virus
-
pH 9.0, 37°C
41933
0.25
RIFGRRSIPVNEQ
Yellow fever virus
-
pH 9.0, 37°C
41928
1.8
SAAQRRGRIGRNQ
Yellow fever virus
-
pH 9.0, 37°C
28606
31.4
SSRKRRSHDVLTQ
Yellow fever virus
-
pH 9.0, 37°C
41927
4.8
VRGARRSGDVLWQ
Yellow fever virus
-
pH 9.0, 37°C
41929
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0034
2-([[3,6-bis(2,6-dimethylphenyl)-7-oxo-2-thioxo-2,3,6,7-tetrahydro[1,3]thiazolo[4,5-d]pyrimidin-5-yl]sulfanyl]methyl)-7-chloro-4H-pyrido[1,2-a]pyrimidin-4-one
-
serotype 4, pH 7.5, 37°C
0.021
4-hydroxypanduratin
-
pH and temperature not specified in the publication
0.021
4-hydroxypanduratin A
-
-
0.0049
4-[3-acetyl-5-(2-phenylquinolin-4-yl)-2,3-dihydro-1,3,4-oxadiazol-2-yl]benzoic acid
-
serotype 4, pH 7.5, 37°C
0.004
5-[(4-chloro-3-nitro-1H-pyrazol-1-yl)methyl]-N-[1-[2-(diethylamino)ethyl]-1H-benzimidazol-2-yl]furan-2-carboxamide
-
serotype 4, pH 7.5, 37°C
0.0667
Ac-EVKKQR-NH2
-
pH 9.0, 37°C
0.0259
Ac-FAAGRK-NH2
-
pH 9.0, 37°C
0.1523
Ac-GKR-NH2
-
pH 9.0, 37°C
0.0223
Ac-KKR-NH2
-
pH 9.0, 37°C
0.1215
Ac-KR-NH2
-
pH 9.0, 37°C
0.0121
Ac-RTSKKR-NH2
-
pH 9.0, 37°C
0.1876
Ac-SKKR-NH2
-
pH 9.0, 37°C
0.046
Ac-TTSTRR-NH2
-
pH 9.0, 37°C
0.0111 - 0.0153
agathisflavone
0.000264
antibody D05320
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.0004
antibody D05321
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.00017
antibody D05322
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.000031
antibody D05323
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.000058
antibody D05444
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.000035
antibody D05445
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.000288
antibody D05446
-
wild type enzyme, in 20 mM Tris/HCl buffer, pH 8.0, at 22°C
-
0.000000079 - 0.000025
Aprotinin
0.00075
Bz-Ala-Lys-Arg-Arg
-
-
0.0053
Bz-Ala-Lys-Arg-Arg-H
-
-
0.00085
Bz-Arg-Arg
-
-
0.012
Bz-Arg-Arg-H
-
-
0.0094
Bz-D-Nle-Lys-Arg-Arg-H
-
-
0.0004
Bz-Lys-Arg-Arg
-
-
0.0015
Bz-Lys-Arg-Arg-H
-
-
0.0437
Bz-N-Me-Nle-Lys-Arg-Arg-H
-
-
0.0013
Bz-Nle-(3-amino)-Phe-(3-imino)-Phe-(3,4-dimethoxy)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0018
Bz-Nle-(3-guanidino)-Phe-(4-guanidino)-Phe-(4-guanidino)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0002
Bz-Nle-(4-amino)-Phe-(3-guanidino)-Phe-(4-amino)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.002
Bz-Nle-(4-amino)-Phe-(3-guanidino)-Phe-Arg
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0017
Bz-Nle-(4-amino)-Phe-(3-imino)-Phe-Arg
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0018
Bz-Nle-(4-amino)-Phe-(4-guanidino)-Phe-Trp
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0009
Bz-Nle-Ala-Arg-Arg
-
-
0.0221
Bz-Nle-Ala-Arg-Arg-H
-
-
0.0015
Bz-Nle-Arg-(3-guanidino)-Phe-(4-amino)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0286
Bz-Nle-D-Lys-Arg-Arg-H
-
-
0.0001
Bz-Nle-His-(3-guanidino)-Phe-(4-guanidino)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0008
Bz-Nle-His-(3-guanidino)-Phe-beta-(2-naphthyl)-Ala
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0017
Bz-Nle-His-(3-imino)-Phe-Arg
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0018
Bz-Nle-His-(4-guanidino)-Phe-(4-guanidino)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0009
Bz-Nle-His-(4-guanidino)-Phe-Trp
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0005
Bz-Nle-homo-His-(3-guanidino)-Phe-(4-guanidino)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0019
Bz-Nle-homoHis-(3-guanidino)-Phe-Arg
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0014
Bz-Nle-homoHis-(4-guanidino)-Phe-(4-phenyl)-Phe
-
predicted inhibition constant, pH and temperature not specified in the publication
0.125
Bz-Nle-Lys-Ala-Arg
-
-
0.5
Bz-Nle-Lys-Ala-Arg-H
-
-
0.138
Bz-Nle-Lys-Arg-(p-Cl)Phe-H
-
-
0.0186
Bz-Nle-Lys-Arg-(p-CN)Phe-H
-
-
0.0028
Bz-Nle-Lys-Arg-(p-guanidinyl)Phe-H
-
-
0.006
Bz-Nle-Lys-Arg-(p-Me)Phe-H
-
-
0.0116
Bz-Nle-Lys-Arg-(p-Ph)Phe-H
-
-
0.03315
Bz-Nle-Lys-Arg-Ala
-
-
0.193
Bz-Nle-Lys-Arg-Ala-H
-
-
0.0004
Bz-Nle-Lys-Arg-Arg
-
-
0.178
Bz-Nle-Lys-Arg-Arg(OH)-CONH-Bn
-
-
0.000043
Bz-Nle-Lys-Arg-Arg-B(OH)2
-
-
0.0829
Bz-Nle-Lys-Arg-Arg-benzoxazole
-
-
0.00085
Bz-Nle-Lys-Arg-Arg-CF3
-
-
0.0058
Bz-Nle-Lys-Arg-Arg-H
-
-
0.1275
Bz-Nle-Lys-Arg-Arg-NH2
-
-
0.5
Bz-Nle-Lys-Arg-Arg-NHSO2CF3
-
-
0.5
Bz-Nle-Lys-Arg-Arg-OH
-
-
0.0428
Bz-Nle-Lys-Arg-Arg-thiazole
-
-
0.051
Bz-Nle-Lys-Arg-D-Arg-H
-
-
0.5
Bz-Nle-Lys-Arg-homoPhe-H
-
-
0.0112
Bz-Nle-Lys-Arg-Lys
-
-
0.0205
Bz-Nle-Lys-Arg-Lys-H
-
-
0.062
Bz-Nle-Lys-Arg-Phe
-
-
0.0159
Bz-Nle-Lys-Arg-Phe-H
-
-
0.033
Bz-Nle-Lys-Arg-Phg-H
-
-
0.0016
Bz-Nle-Lys-Arg-Trp
-
predicted inhibition constant, pH and temperature not specified in the publication
0.0075
Bz-Nle-Lys-Arg-Trp-H
-
-
0.115
Bz-Nle-Lys-D-Arg-Arg-H
-
-
0.0012
Bz-Nle-Lys-Lys-Arg
-
-
0.00005
Bz-Nle-Lys-Lys-Arg-B(OH)2
-
-
0.0413
Bz-Nle-Lys-Lys-Arg-H
-
-
0.0474
Bz-Nle-Lys-N-Me-Arg-Arg-H
-
-
0.0156
Bz-Nle-Lys-Phe-Arg
-
-
0.0407
Bz-Nle-Lys-Phe-Arg-H
-
-
0.109
Bz-Nle-Lys-Pro-Arg-H
-
-
0.5
Bz-Nle-Lys-Thr-Arg-H
-
-
0.5
Bz-Nle-Lys-Thr-Arg-NH2
-
-
0.5
Bz-Nle-Lys-Thr-Arg-OH
-
-
0.1133
Bz-Nle-N-Me-Lys-Arg-Arg-H
-
-
0.0015
Bz-Nle-Phe-Arg-Arg
-
-
0.0158
Bz-Nle-Phe-Arg-Arg-H
-
-
0.0614
Bz-Nle-Pro-Arg-Arg-H
-
-
0.00025
Bz-Phe-Lys-Arg-Arg
-
-
0.0068
Bz-Phe-Lys-Arg-Arg-H
-
-
0.377
cardamonin
-
-
0.0004
decanoyl-Arg-Val-Lys-Arg-chloromethylketone
-
-
0.0047 - 0.0257
myricetin
0.0025
N2-(biphenyl-3-ylacetyl)-L-lysyl-N-[(1-carbamimidoylpiperidin-4-yl)methyl]-L-lysinamide
-
pH 8.0, 25°C
0.000006
nona-D-Arg-amide
-
-
0.025
panduratin A
0.345
pinostrobin
-
-
0.0199 - 0.0227
quercetin
0.01984
[4-methyl-5-(3-methylbut-2-en-1-yl)-6-phenylcyclohex-3-en-1-yl](2,4,6-trimethoxyphenyl)methanone
-
pH and temperature not specified in the publication
-
0.02436
[5-(3-methylbut-2-en-1-yl)-6-phenylcyclohex-3-en-1-yl](2,4,6-trimethoxyphenyl)methanone
-
pH and temperature not specified in the publication
-
0.03968
[6-(4-methoxyphenyl)-4-methyl-5-(3-methylbut-2-en-1-yl)cyclohex-3-en-1-yl](2,4,6-trimethoxyphenyl)methanone
-
pH and temperature not specified in the publication
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001263
(5-amino-1-phenylsulfonylpyrazol-3-yl) 2-bromobenzoate
West Nile virus
-
-
0.002376
(5-amino-1-phenylsulfonylpyrazol-3-yl)thiophene-2-carboxylate
West Nile virus
-
-
0.0036
(E)-N-(5-(benzylsulfanyl)-1,3,4-thiadiazol-2-yl)-2-cyano-3-(1-((2-fluorophenyl)methyl)-1H-indol-3-yl)prop-2-enamide
Dengue virus
-
pH 8.5, 37°C
0.0022
(E)-N-(5-(benzylsulfanyl)-1,3,4-thiadiazol-2-yl)-2-cyano-3-(1-((4-fluorophenyl)methyl)-1H-indol-3-yl)prop-2-enamide
Dengue virus
-
pH 8.5, 37°C
0.89 - 5.9
1,10-phenanthroline
0.1
1-(2,6-difluorophenyl)-2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]ethanol
West Nile virus
-
IC50 above 0.1 mM, in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.0239
1-(2,6-difluorophenyl)-2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]ethanone
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.0388
1-(2-fluorophenyl)-2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]ethanone
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.00926
1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl 2,6-difluoro-3-methylbenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.00871
1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl 2,6-difluorobenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.00403
1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl benzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.00943
1-benzyl-3-methyl-1H-pyrazol-5-yl 2,6-difluoro-3-methylbenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.00804
1-benzyl-3-methyl-1H-pyrazol-5-yl 2,6-difluorobenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.0039
2-([[3,6-bis(2,6-dimethylphenyl)-7-oxo-2-thioxo-2,3,6,7-tetrahydro[1,3]thiazolo[4,5-d]pyrimidin-5-yl]sulfanyl]methyl)-7-chloro-4H-pyrido[1,2-a]pyrimidin-4-one
Dengue virus
-
serotype 4, pH 7.5, 37°C
0.0018 - 0.0267
2-naphthoyl-Lys-Arg-Arg
0.1
2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]-1-phenylethanol
West Nile virus
-
IC50 above 0.1 mM, in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
-
0.1
2-[1-(4-methoxybenzyl)-3-methyl-1H-pyrazol-5-yl]-1-phenylethanone
West Nile virus
-
IC50 above 0.1 mM, in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.03409
2-[[(4-methoxyphenyl)amino]methylidene]cyclohexane-1,3-dione
West Nile virus
-
-
0.0224 - 0.201
4-aminobenzoyl-Lys-Arg-Arg
0.0335 - 0.3
4-aminobenzoyl-Lys-Lys-Arg
0.0019 - 0.0112
4-aminophenylacetyl-Lys-Arg-Arg
0.00099 - 0.0234
4-phenylphenylacetyl-Lys-Arg-Arg
0.000056 - 0.0122
4-phenylphenylacetyl-Lys-Lys-Arg
0.0125
4-[3-acetyl-5-(2-phenylquinolin-4-yl)-2,3-dihydro-1,3,4-oxadiazol-2-yl]benzoic acid
Dengue virus
-
serotype 4, pH 7.5, 37°C
0.01192
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,3,4-trifluorobenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.01069
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,3,5,6-tetrafluoro-4-methylbenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35
0.00097
5-amino-1-[(4-methoxyphenyl)sulfonyl]-1H-pyrazol-3-yl 2,3,6-trifluorobenzoate
West Nile virus
-
in 10 mM Tris-HCl buffer, pH 8.0, containing 20% (v/v) glycerol and 0.005% Brij 35