Cloned (Comment) | Organism |
---|---|
complete NS3 molecule fused to 18 residues of the NS2B cofactor or NS3 helicase (residues 177 to 618) cloned into pET32b vector and expressed in Escherichia coli BL21-CodonPlus | Dengue virus type 4 |
Crystallization (Comment) | Organism |
---|---|
complete NS3 molecule fused to 18 residues of the NS2B cofactor, by hanging-drop vapor diffusion method, at 3.15 A resolution. Protease domain sits beneath the ATP binding site, giving the molecule an elongated shape. Domain arrangement in the crystal structure fits into an envelope, suggesting a stable molecular conformation. A basic patch located at the surface of the protease domain increases the affinity for nucleotides and may also participate in RNA binding, explaining the higher unwinding activity of the full-length enzyme compared to that of the isolated helicase domain | Dengue virus type 4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Dengue virus type 4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
1 * 74000, SAXS experiments with the complete NS3 molecule fused to 18 residues of the NS2B cofactor | Dengue virus type 4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus type 4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
complete NS3 molecule fused to 18 residues of the NS2B cofactor or NS3 helicase (residues 177 to 618) purified by gel filtration | Dengue virus type 4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP ATOO-647N + H2O | affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase | Dengue virus type 4 | ? | - |
? | |
ATP ATOO-647N + H2O | affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase | Dengue virus type 4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 74000, SAXS experiments with the complete NS3 molecule fused to 18 residues of the NS2B cofactor | Dengue virus type 4 |
Synonyms | Comment | Organism |
---|---|---|
nonstructural protein 3 | - |
Dengue virus type 4 |
NS3 | - |
Dengue virus type 4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NS2B protein | - |
Dengue virus type 4 |