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Literature summary for 3.4.21.91 extracted from

  • Luo, D.; Xu, T.; Hunke, C.; Grueber, G.; Vasudevan, S.G.; Lescar, J.
    Crystal structure of the NS3 protease-helicase from dengue virus (2008), J. Virol., 82, 173-183.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
complete NS3 molecule fused to 18 residues of the NS2B cofactor or NS3 helicase (residues 177 to 618) cloned into pET32b vector and expressed in Escherichia coli BL21-CodonPlus Dengue virus type 4

Crystallization (Commentary)

Crystallization (Comment) Organism
complete NS3 molecule fused to 18 residues of the NS2B cofactor, by hanging-drop vapor diffusion method, at 3.15 A resolution. Protease domain sits beneath the ATP binding site, giving the molecule an elongated shape. Domain arrangement in the crystal structure fits into an envelope, suggesting a stable molecular conformation. A basic patch located at the surface of the protease domain increases the affinity for nucleotides and may also participate in RNA binding, explaining the higher unwinding activity of the full-length enzyme compared to that of the isolated helicase domain Dengue virus type 4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Dengue virus type 4

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
1 * 74000, SAXS experiments with the complete NS3 molecule fused to 18 residues of the NS2B cofactor Dengue virus type 4

Organism

Organism UniProt Comment Textmining
Dengue virus type 4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
complete NS3 molecule fused to 18 residues of the NS2B cofactor or NS3 helicase (residues 177 to 618) purified by gel filtration Dengue virus type 4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP ATOO-647N + H2O affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase Dengue virus type 4 ?
-
?
ATP ATOO-647N + H2O affinity of complete NS3 molecule fused to 18 residues of the NS2B cofactor is 10fold higher than that of NS3 helicase Dengue virus type 4 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 74000, SAXS experiments with the complete NS3 molecule fused to 18 residues of the NS2B cofactor Dengue virus type 4

Synonyms

Synonyms Comment Organism
nonstructural protein 3
-
Dengue virus type 4
NS3
-
Dengue virus type 4

Cofactor

Cofactor Comment Organism Structure
NS2B protein
-
Dengue virus type 4