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Literature summary for 3.4.21.91 extracted from

  • Loehr, K.; Knox, J.E.; Phong, W.Y.; Ma, N.L.; Yin, Z.; Sampath, A.; Patel, S.J.; Wang, W.L.; Chan, W.L.; Rao, K.R.; Wang, G.; Vasudevan, S.G.; Keller, T.H.; Lim, S.P.
    Yellow fever virus NS3 protease: peptide-inhibition studies (2007), J. Gen. Virol., 88, 2223-2227.
    View publication on PubMed

Application

Application Comment Organism
drug development characteristics of Yellow fever virus protease are very similar to those reported for dengue and West Nile virus proteases, and suggest that pan-flavivirus NS3 protease drugs may be developed for flaviviral diseases Yellow fever virus

Cloned(Commentary)

Cloned (Comment) Organism
single-chain complex of NS2B/NS3 protease (CF40-gly-NS3pro190) expressed as an N-terminally His-tagged fusion protein in Escherichia coli Yellow fever virus

Protein Variants

Protein Variants Comment Organism
S138A is inactive towards Bz-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin Yellow fever virus

Inhibitors

Inhibitors Comment Organism Structure
Aprotinin
-
Yellow fever virus
Bz-Ala-Lys-Arg-Arg
-
Yellow fever virus
Bz-Arg-Arg
-
Yellow fever virus
Bz-Lys-Arg-Arg
-
Yellow fever virus
Bz-Nle-Ala-Arg-Arg
-
Yellow fever virus
Bz-Nle-Lys-Ala-Arg
-
Yellow fever virus
Bz-Nle-Lys-Arg-Ala
-
Yellow fever virus
Bz-Nle-Lys-Arg-Arg
-
Yellow fever virus
Bz-Nle-Lys-Arg-Lys
-
Yellow fever virus
Bz-Nle-Lys-Arg-Phe
-
Yellow fever virus
Bz-Nle-Lys-Lys-Arg
-
Yellow fever virus
Bz-Nle-Lys-Lys-Arg-B(OH)2
-
Yellow fever virus
Bz-Nle-Lys-Phe-Arg
-
Yellow fever virus
Bz-Nle-Phe-Arg-Arg
-
Yellow fever virus
Bz-Phe-Lys-Arg-Arg
-
Yellow fever virus
additional information inhibitor potency is driven predominantely by interactions with amino acid residues in the S1 and S2 pockets, as substitutions in the P1 and P2 sites have the greates impact on inhibitor potency. P1 and P2 positions are most important for inhibitor binding, whilst the P3 and P4 positions have much less effect Yellow fever virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0146
-
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin
-
Yellow fever virus
0.1422
-
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin
-
Yellow fever virus

Organism

Organism UniProt Comment Textmining
Yellow fever virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on Ni2+ affinity column Yellow fever virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
Yellow fever virus ?
-
?
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O 10fold higher affinity for the tetrapeptide than for the tripeptide Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin, and processes it with 34fold greater efficiency Yellow fever virus ?
-
?

Synonyms

Synonyms Comment Organism
NS2B-NS3 proteinase
-
Yellow fever virus
NS3 protease
-
Yellow fever virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.034
-
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin
-
Yellow fever virus
0.111
-
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin
-
Yellow fever virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Yellow fever virus

Cofactor

Cofactor Comment Organism Structure
NS2B protein
-
Yellow fever virus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00001761
-
Aprotinin
-
Yellow fever virus
0.00005
-
Bz-Nle-Lys-Lys-Arg-B(OH)2
-
Yellow fever virus
0.00025
-
Bz-Phe-Lys-Arg-Arg
-
Yellow fever virus
0.0004
-
Bz-Nle-Lys-Arg-Arg
-
Yellow fever virus
0.0004
-
Bz-Lys-Arg-Arg
-
Yellow fever virus
0.00075
-
Bz-Ala-Lys-Arg-Arg
-
Yellow fever virus
0.00085
-
Bz-Arg-Arg
-
Yellow fever virus
0.0009
-
Bz-Nle-Ala-Arg-Arg
-
Yellow fever virus
0.0012
-
Bz-Nle-Lys-Lys-Arg
-
Yellow fever virus
0.0015
-
Bz-Nle-Phe-Arg-Arg
-
Yellow fever virus
0.0112
-
Bz-Nle-Lys-Arg-Lys
-
Yellow fever virus
0.0156
-
Bz-Nle-Lys-Phe-Arg
-
Yellow fever virus
0.03315
-
Bz-Nle-Lys-Arg-Ala
-
Yellow fever virus
0.062
-
Bz-Nle-Lys-Arg-Phe
-
Yellow fever virus
0.125
-
Bz-Nle-Lys-Ala-Arg
-
Yellow fever virus