Application | Comment | Organism |
---|---|---|
drug development | characteristics of Yellow fever virus protease are very similar to those reported for dengue and West Nile virus proteases, and suggest that pan-flavivirus NS3 protease drugs may be developed for flaviviral diseases | Yellow fever virus |
Cloned (Comment) | Organism |
---|---|
single-chain complex of NS2B/NS3 protease (CF40-gly-NS3pro190) expressed as an N-terminally His-tagged fusion protein in Escherichia coli | Yellow fever virus |
Protein Variants | Comment | Organism |
---|---|---|
S138A | is inactive towards Bz-Nle-Lys-Arg-Arg-7-amino-4-methylcoumarin | Yellow fever virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aprotinin | - |
Yellow fever virus | |
Bz-Ala-Lys-Arg-Arg | - |
Yellow fever virus | |
Bz-Arg-Arg | - |
Yellow fever virus | |
Bz-Lys-Arg-Arg | - |
Yellow fever virus | |
Bz-Nle-Ala-Arg-Arg | - |
Yellow fever virus | |
Bz-Nle-Lys-Ala-Arg | - |
Yellow fever virus | |
Bz-Nle-Lys-Arg-Ala | - |
Yellow fever virus | |
Bz-Nle-Lys-Arg-Arg | - |
Yellow fever virus | |
Bz-Nle-Lys-Arg-Lys | - |
Yellow fever virus | |
Bz-Nle-Lys-Arg-Phe | - |
Yellow fever virus | |
Bz-Nle-Lys-Lys-Arg | - |
Yellow fever virus | |
Bz-Nle-Lys-Lys-Arg-B(OH)2 | - |
Yellow fever virus | |
Bz-Nle-Lys-Phe-Arg | - |
Yellow fever virus | |
Bz-Nle-Phe-Arg-Arg | - |
Yellow fever virus | |
Bz-Phe-Lys-Arg-Arg | - |
Yellow fever virus | |
additional information | inhibitor potency is driven predominantely by interactions with amino acid residues in the S1 and S2 pockets, as substitutions in the P1 and P2 sites have the greates impact on inhibitor potency. P1 and P2 positions are most important for inhibitor binding, whilst the P3 and P4 positions have much less effect | Yellow fever virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0146 | - |
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin | - |
Yellow fever virus | |
0.1422 | - |
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin | - |
Yellow fever virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yellow fever virus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni2+ affinity column | Yellow fever virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Yellow fever virus | ? | - |
? | |
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin + H2O | 10fold higher affinity for the tetrapeptide than for the tripeptide Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin, and processes it with 34fold greater efficiency | Yellow fever virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NS2B-NS3 proteinase | - |
Yellow fever virus |
NS3 protease | - |
Yellow fever virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
Boc-Gly-Arg-Arg-7-amido-4-methylcoumarin | - |
Yellow fever virus | |
0.111 | - |
Bz-Nle-Lys-Arg-Arg-7-amido-4-methylcoumarin | - |
Yellow fever virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Yellow fever virus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NS2B protein | - |
Yellow fever virus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00001761 | - |
Aprotinin | - |
Yellow fever virus | |
0.00005 | - |
Bz-Nle-Lys-Lys-Arg-B(OH)2 | - |
Yellow fever virus | |
0.00025 | - |
Bz-Phe-Lys-Arg-Arg | - |
Yellow fever virus | |
0.0004 | - |
Bz-Nle-Lys-Arg-Arg | - |
Yellow fever virus | |
0.0004 | - |
Bz-Lys-Arg-Arg | - |
Yellow fever virus | |
0.00075 | - |
Bz-Ala-Lys-Arg-Arg | - |
Yellow fever virus | |
0.00085 | - |
Bz-Arg-Arg | - |
Yellow fever virus | |
0.0009 | - |
Bz-Nle-Ala-Arg-Arg | - |
Yellow fever virus | |
0.0012 | - |
Bz-Nle-Lys-Lys-Arg | - |
Yellow fever virus | |
0.0015 | - |
Bz-Nle-Phe-Arg-Arg | - |
Yellow fever virus | |
0.0112 | - |
Bz-Nle-Lys-Arg-Lys | - |
Yellow fever virus | |
0.0156 | - |
Bz-Nle-Lys-Phe-Arg | - |
Yellow fever virus | |
0.03315 | - |
Bz-Nle-Lys-Arg-Ala | - |
Yellow fever virus | |
0.062 | - |
Bz-Nle-Lys-Arg-Phe | - |
Yellow fever virus | |
0.125 | - |
Bz-Nle-Lys-Ala-Arg | - |
Yellow fever virus |