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3.4.21.9: enteropeptidase

This is an abbreviated version!
For detailed information about enteropeptidase, go to the full flat file.

Word Map on EC 3.4.21.9

Reaction

Activation of trypsinogen by selective cleavage of Lys6-/-Ile bond =

Synonyms

BEK, BEP, bovine enterokinase light chain, bovine enteropeptidase, Chinese bovine enterokinase, Chinese northern yellow bovine enterokinase catalytic subunit, EC 3.4.4.8, EK, EKL, EKL-His6, EKLC, enterokinase, enterokinase light chain, enteropeptidase, enteropeptidase light chain, EP 118-1035, EP-1, EPL, HEK, HEP, human enteropeptidase, L-BEP, L-HEK, L-HEP, native enterokinase, natural enteropeptidase, peptidase, entero-, porcine enterokinase, PRSS7, recombinant bovine enterokinase catalytic subunit protein, recombinant enterokinase light chain, recombinant His-tagged enterokinase light chain, rEKL, rEKL/His, sBEKLC, TMPRSS15

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.9 enteropeptidase

Cloned

Cloned on EC 3.4.21.9 - enteropeptidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
catalytic subunit is expressed in escherichia coli BL21
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells with maltose binding protein tag
expressed in Escherichia coli BL21(DE3) codon plus RIL hEPL sc C122S cells
expressed in Escherichia coli strain BL21 (DE3)
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expressed in Escherichia coli strain BL21(DE3) and on M13 filamentous bacteriophage
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expressed in Pichia pastoris
expressed in Pichia pastoris methylotrophic strain GS115
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expressed in Pichia pastoris strain GS115 mutants Mut+ and MutS
-
expressed in Pichia pastoris strain Y11430
expressed in Pichia pastoris X-33 strain
expressed in Sf-9 insect cells
expression as fusion protein with thioredoxin in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli as glutathione S-transferase-fusion protein, the gene is a Pro82Arg/Glu176Asp variant of the known bovine EK
expression in Escherichia coli BL21
-
expression of a 26300 Da protein containing the catalytic domain of bovine enterokinase, expression in methylotrophic yeast Pichia pastoris
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expression of catalytic light subunit in Escherichia coli
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expression of light chain of enteropeptidase in Escherichia coli BL21(DE3) in both soluble and insoluble form
expression of the enterokinase light chain fused to DsbA in Escherichia coli
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expression of wild-type and mutant enterokinase light chain in Escherichia coli strain Rosetta (DE3)
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recombinant expression of enteropeptidase light chain as thioredoxin-fusion protein in Escherichia coli strain BL21(DE3) in aggregated form, subcloning in Escherichia coli strain DH5alpha
the synthetic gene encoding human enteropeptidase light chain with His-tag added at the C-terminus to facilitate protein purification is cloned into Pichia pastoris expression plasmids under the control of an inducible AOX1 or constitutive promoters GAP and AAC