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Literature summary for 3.4.21.9 extracted from

  • Jirickova, K.; Gal, M.; Krahulec, J.; Hives, J.
    Electrochemical determination of basic biochemical properties of enzyme enterokinase (2015), Monatsh. Chem., 146, 755-759.
No PubMed abstract available
Search for more literature for 3.4.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.123
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by fluorescence spectroscopy, pH not specified in the publication, at 37°C Homo sapiens
0.143
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by impedance spectroscopy, pH not specified in the publication, at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
trypsinogen + H2O Homo sapiens
-
 trypsin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P98073
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
 Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
trypsinogen + H2O
-
 Homo sapiens trypsin + ?
-
 ?

Synonyms

Synonyms Comment Organism
enterokinase
-
 Homo sapiens
L-HEK catalytic subunit Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
98
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by fluorescence spectroscopy, pH not specified in the publication, at 37°C Homo sapiens
121
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by impedance spectroscopy, pH not specified in the publication, at 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
805
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by fluorescence spectroscopy, pH not specified in the publication, at 37°C Homo sapiens
846
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide value obtained by impedance spectroscopy, pH not specified in the publication, at 37°C Homo sapiens