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Literature summary for 3.4.21.9 extracted from

  • Gasparian, M.E.; Ostapchenko, V.G.; Schulga, A.A.; Dolgikh, D.A.; Kirpichnikov, M.P.
    Expression, purification, and characterization of human enteropeptidase catalytic subunit in Escherichia coli (2003), Protein Expr. Purif., 31, 133-139.
    View publication on PubMed
Search for more literature for 3.4.21.9

Application

Application Comment Organism
synthesis the enzyme can be used for cleavage of fusion proteins due to its high specific activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of light chain of enteropeptidase in Escherichia coli BL21(DE3) in both soluble and insoluble form Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Soybean trypsin inhibitor
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 37°C Homo sapiens
0.16
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P98073 light chain
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fusion protein Trx/hEGF + H2O the fusion protein Trx/hEGF contains an inter-domain enteropeptidase recognition site Homo sapiens ?
-
 ?
fusion protein Trx/hIL-13 + H2O the fusion protein Trx/hIL-13 contains an inter-domain enteropeptidase recognition site Homo sapiens ?
-
 ?
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
 Homo sapiens Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
thiobenzyl benzyloxy-carbonyl-L-lysinate + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
115
-
 Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide pH 8.4, 37°C Homo sapiens
133
-
 thiobenzyl benzyloxy-carbonyl-L-lysinate pH 8.4, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000023
-
 Soybean trypsin inhibitor pH 8.4, 37°C Homo sapiens