3.4.21.9: enteropeptidase

This is an abbreviated version!
For detailed information about enteropeptidase, go to the full flat file.

Reaction

Activation of trypsinogen by selective cleavage of Lys6-/-Ile bond =

Synonyms

BEK, BEP, bovine enterokinase light chain, bovine enteropeptidase, Chinese bovine enterokinase, Chinese northern yellow bovine enterokinase catalytic subunit, EC 3.4.4.8, EK, EKL, EKL-His6, EKLC, enterokinase, enterokinase light chain, enteropeptidase, enteropeptidase light chain, EP 118-1035, EP-1, EPL, HEK, HEP, human enteropeptidase, L-BEP, L-HEK, L-HEP, native enterokinase, natural enteropeptidase, peptidase, entero-, porcine enterokinase, PRSS7, recombinant bovine enterokinase catalytic subunit protein, recombinant enterokinase light chain, recombinant His-tagged enterokinase light chain, rEKL, rEKL/His, sBEKLC, TMPRSS15

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.9 enteropeptidase

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Results	in table
2	Activating Compound
84	AA Sequence
19	Application
1	CAS Registry Number
26	Cloned(Commentary)
3	Crystallization (Commentary)
420	Disease/ Diagnostics
10	General Information
2	General Stability
40	Inhibitors
39	kcat/KM [mM/s]
4	Ki Value [mM]
139	KM Value [mM]
7	Localization
11	Metals/Ions
25	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
74	Organism
11	PDB ID
18	pH Optimum
6	pH Range
14	Posttranslational Modification
31	Protein Variants
32	Purification (Commentary)
1	Reaction
12	Reaction Type
74	Reference
3	Renatured (Commentary)
35	Source Tissue
14	Specific Activity [micromol/min/mg]
4	Storage Stability
250	Substrates and Products (Substrate)
20	Subunits
73	Synonyms
9	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
109	Turnover Number [1/s]

KCat KM Value

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of the Enzyme Summary Page.

KCat KM Value on EC 3.4.21.9 - enteropeptidase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.22 - 1.81

benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin

5 entries

137 - 309

formyl-Ala-Phe-Arg-4-nitroanilide

2 entries

55 - 109

formyl-Ala-Phe-Lys-4-nitroanilide

2 entries

42

GD4K 2-naphthylamide

Homo sapiens

-

pH 8.4, 35°C

717431

2623

GD4K 7-amido-4-methylcoumarin

Homo sapiens

-

pH 8.4, 35°C

717431

2330 - 18900

GD4R-4-nitroanilide

3 entries

29.6 - 6052

GDDDDK-2-naphthylamide

7 entries

537 - 6830

GDDDDK-4-nitroanilide

3 entries

347 - 3500

Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide

4 entries

540.56

Gly-L-Asp-L-Asp-L-Asp-L-Asp-L-Lys-2-naphthylamide

Homo sapiens

P98073

at pH 8.0 and 37°C

752564

0.23 - 2.29

t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin

5 entries

950 - 969

thiobenzyl benzyloxy-carbonyl-L-lysinate

2 entries

1770 - 2150

Z-Lys-SBzl

3 entries

0.22

benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme E136Y, at pH 8.3 and 30°C

732855

0.41

benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

wild type enzyme, at pH 8.3 and 30°C

732855

0.62

benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme R213L, at pH 8.3 and 30°C

732855

0.89

benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme H24Q, at pH 8.3 and 30°C

732855

1.81

benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme E136Y/R213L, at pH 8.3 and 30°C

732855

137

formyl-Ala-Phe-Arg-4-nitroanilide

Bos taurus

-

pH 8.4, 25°C

718359

309

formyl-Ala-Phe-Arg-4-nitroanilide

Homo sapiens

-

pH 8.4, 25°C

718359

55

formyl-Ala-Phe-Lys-4-nitroanilide

Bos taurus

-

pH 8.4, 25°C

718359

109

formyl-Ala-Phe-Lys-4-nitroanilide

Homo sapiens

-

pH 8.4, 25°C

718359

2330

GD4R-4-nitroanilide

Homo sapiens

P98073

mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

7200

GD4R-4-nitroanilide

Homo sapiens

P98073

wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

18900

GD4R-4-nitroanilide

Homo sapiens

P98073

mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

29.6

GDDDDK-2-naphthylamide

Oryzias latipes

A4UWM5

mutant enzyme E136Y, at pH 8.3 and 30°C

732855

43.63

GDDDDK-2-naphthylamide

Oryzias latipes

A4UWM5

wild type enzyme, at pH 8.3 and 30°C

732855

61.1

GDDDDK-2-naphthylamide

Oryzias latipes

A4UWM5

mutant enzyme R213L, at pH 8.3 and 30°C

732855

63.1

GDDDDK-2-naphthylamide

Oryzias latipes

A4UWM5

mutant enzyme H24Q, at pH 8.3 and 30°C

732855

99.5

GDDDDK-2-naphthylamide

Oryzias latipes

A4UWM5

mutant enzyme E136Y/R213L, at pH 8.3 and 30°C

732855

4600

GDDDDK-2-naphthylamide

Homo sapiens

-

soluble enzyme, in 25 mM Tris-HCl (pH 8.4), at 37°C

731383

6052

GDDDDK-2-naphthylamide

Homo sapiens

-

phage-bound enteropeptidase catalytic subunit, in 25 mM Tris-HCl (pH 8.4), at 37°C

731383

537

GDDDDK-4-nitroanilide

Homo sapiens

P98073

mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

4530

GDDDDK-4-nitroanilide

Homo sapiens

P98073

wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

6830

GDDDDK-4-nitroanilide

Homo sapiens

P98073

mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

347

Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide

Bos taurus

-

pH 8.4, 37°C

718359

805

Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide

Homo sapiens

P98073

value obtained by fluorescence spectroscopy, pH not specified in the publication, at 37°C

732551

846

Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide

Homo sapiens

P98073

value obtained by impedance spectroscopy, pH not specified in the publication, at 37°C

732551

3500

Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide

Homo sapiens

-

pH 8.4, 37°C

718359

0.23

t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme E136Y, at pH 8.3 and 30°C

732855

0.23

t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

wild type enzyme, at pH 8.3 and 30°C

732855

0.67

t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme R213L, at pH 8.3 and 30°C

732855

0.85

t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme H24Q, at pH 8.3 and 30°C

732855

2.29

t-butyloxycarbonyl-E(benzyl ester)-Ala-Arg-7-amido-4-methylcoumarin

Oryzias latipes

A4UWM5

mutant enzyme E136Y/R213L, at pH 8.3 and 30°C

732855

950

thiobenzyl benzyloxy-carbonyl-L-lysinate

Homo sapiens

-

pH 8.4, 25°C

718359

969

thiobenzyl benzyloxy-carbonyl-L-lysinate

Bos taurus

-

pH 8.4, 25°C

718359

1770

Z-Lys-SBzl

Homo sapiens

P98073

wild type enzyme, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

1860

Z-Lys-SBzl

Homo sapiens

P98073

mutant enzyme Y174R, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883

2150

Z-Lys-SBzl

Homo sapiens

P98073

mutant enzyme R96Q, in 100 mM Tris, 0.02 mM CaCl2, 0.01% (v/v) Triton X-100, 10% (v/v) DMSO, pH 8.0, at 22°C

732883