3.4.21.9: enteropeptidase

This is an abbreviated version!
For detailed information about enteropeptidase, go to the full flat file.

Reaction

Activation of trypsinogen by selective cleavage of Lys6-/-Ile bond =

Synonyms

BEK, BEP, bovine enterokinase light chain, bovine enteropeptidase, Chinese bovine enterokinase, Chinese northern yellow bovine enterokinase catalytic subunit, EC 3.4.4.8, EK, EKL, EKL-His6, EKLC, enterokinase, enterokinase light chain, enteropeptidase, enteropeptidase light chain, EP 118-1035, EP-1, EPL, HEK, HEP, human enteropeptidase, L-BEP, L-HEK, L-HEP, native enterokinase, natural enteropeptidase, peptidase, entero-, porcine enterokinase, PRSS7, recombinant bovine enterokinase catalytic subunit protein, recombinant enterokinase light chain, recombinant His-tagged enterokinase light chain, rEKL, rEKL/His, sBEKLC, TMPRSS15

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.9 enteropeptidase

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Results	in table
2	Activating Compound
84	AA Sequence
19	Application
1	CAS Registry Number
26	Cloned(Commentary)
3	Crystallization (Commentary)
420	Disease/ Diagnostics
10	General Information
2	General Stability
40	Inhibitors
39	kcat/KM [mM/s]
4	Ki Value [mM]
139	KM Value [mM]
7	Localization
11	Metals/Ions
25	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
74	Organism
11	PDB ID
18	pH Optimum
6	pH Range
14	Posttranslational Modification
31	Protein Variants
32	Purification (Commentary)
1	Reaction
12	Reaction Type
74	Reference
3	Renatured (Commentary)
35	Source Tissue
14	Specific Activity [micromol/min/mg]
4	Storage Stability
250	Substrates and Products (Substrate)
20	Subunits
73	Synonyms
9	Temperature Optimum [°C]
2	Temperature Range [°C]
5	Temperature Stability [°C]
109	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

fast dilution method in 0.7 M arginine, 1 mM EDTA, 3 mM reduced glutathione, and 3 mM GSSG at pH 8.6

Homo sapiens

P98073

732888

fast dilution method in 20 mM NH3 and 10% (v/v) glycerol at pH 10.5 for 72 h at 4?

Oryzias latipes

A4UWM5

732855

recombinant expression of thioredoxin-fusion enteropeptidase light chain from aggregates in 3 M guanidine-HCl, pH 8.0, in Escherichia coli strain BL21(DE3) by dilution of the protein in 0.7 M arginine-HCl, pH 8.5, 15% v/v glycerol, 3 mM reduced glutathione, and 1 mM EDTA. After 72 h at 4°C the refolding solution is dialyzed for 8 h against 50 mM Tris-HCl, pH 8.0, to facilitate complete autocatalytic activation by cleaving the fusion tag

Homo sapiens

P98073

718352