Application | Comment | Organism |
---|---|---|
additional information | utility of enterokinase light chain as a site-specific cleavage enzyme is hampered by sporadic cleavage at other sites than the canonical D4K recognition sequence | Bos taurus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enterokinase light chain in Escherichia coli strain Rosetta (DE3) | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | in order to produce more site-specific enterokinase light chain, EKL, several mutants are generated with substitutions at Tyr174 and Lys99 using the protein disulfide isomerase fusion system. Substitution of Tyr174 by basic residues confers higher specificity on EKL. Mutant enzyme substrate specificity and modeling, overview | Bos taurus |
Y174K | site-directed mutagenesis, | Bos taurus |
Y174K/K99M | site-directed mutagenesis, the mutant does not form the active structure | Bos taurus |
Y174K/K99Q | site-directed mutagenesis, the mutantion results in a more site-specific enterokinase light chain | Bos taurus |
Y174R | site-directed mutagenesis, | Bos taurus |
Y174R/K99M | site-directed mutagenesis, the mutant does not form the active structure | Bos taurus |
Y174R/K99Q | site-directed mutagenesis, the mutant does not form the active structure | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Bos taurus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | enterokinase light chain is a serine protease that recognizes Asp-Asp-Asp-Asp-Lys, D4K, sequence and cleaves the C-terminal peptide bond of the lysine residue | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | mucosa | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O | - |
Bos taurus | Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine | - |
? | |
Gly-Asp-Asp-Asp-Asp-Lys-Ile-Val-Gly-Gly + H2O | - |
Bos taurus | Gly-Asp-Asp-Asp-Asp-Lys + Ile-Val-Gly-Gly | - |
? | |
Gly-Gly-Asp-Asp-Asp-Lys-Ile-Val-Gly-Gly + H2O | - |
Bos taurus | Gly-Gly-Asp-Asp-Asp-Lys + Ile-Val-Gly-Gly | - |
? | |
Gly-Gly-Gly-Asp-Asp-Lys-Ile-Val-Gly-Gly + H2O | - |
Bos taurus | Gly-Gly-Gly-Asp-Asp-Lys + Ile-Val-Gly-Gly | - |
? | |
Gly-Gly-Gly-Gly-Asp-Lys-Ile-Val-Gly-Gly + H2O | - |
Bos taurus | Gly-Gly-Gly-Gly-Asp-Lys + Ile-Val-Gly-Gly | - |
? | |
additional information | enterokinase light chain is a serine protease that recognizes Asp-Asp-Asp-Asp-Lys, D4K, sequence and cleaves the C-terminal peptide bond of the lysine residue | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enterokinase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bos taurus |
General Information | Comment | Organism |
---|---|---|
additional information | enterokinase shows no strict cleavage site specificity because of sporadic cleavage at other sites, which consist of acidic and basic residues | Bos taurus |