Application | Comment | Organism |
---|---|---|
molecular biology | the high specificity of the target site makes enterokinase an ideal tool for cleaving fusion proteins at defined cleavage sites | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
the synthetic gene encoding human enteropeptidase light chain with His-tag added at the C-terminus to facilitate protein purification is cloned into Pichia pastoris expression plasmids under the control of an inducible AOX1 or constitutive promoters GAP and AAC | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
x * 27000, deglycosylated recombinant enzyme, SDS-PAGE, x * 37000-72000, hyperglycosylated recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | Homo sapiens | activation by by endoproteolytic cleavage | trypsin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P98073 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | enterokinase is expressed in the proximal part, the heavy chain anchors the enzyme in the intestinal brush border membrane | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
trypsinogen + H2O | activation by by endoproteolytic cleavage | Homo sapiens | trypsin | - |
? | |
trypsinogen + H2O | the enzyme cleaves after lysine residue if the Lys is preceded by four Asp and not followed by a Pro, and it shows high specificity of the target site. The light chain represents the catalytic enzyme subunit | Homo sapiens | trypsin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27000, deglycosylated recombinant enzyme, SDS-PAGE, x * 37000-72000, hyperglycosylated recombinant enzyme, SDS-PAGE | Homo sapiens |
More | the mature active enzyme is comprised of two disulfide-linked polypeptide chains. The heavy chain anchors the enzyme in the intestinal brush border membrane, whereas the light chain represents the catalytic enzyme subunit | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
enterokinase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme converts the inactive form of trypsinogen to active trypsin by endoproteolytic cleavage | Homo sapiens |