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3.4.21.9: enteropeptidase

This is an abbreviated version!
For detailed information about enteropeptidase, go to the full flat file.

Word Map on EC 3.4.21.9

Reaction

Activation of trypsinogen by selective cleavage of Lys6-/-Ile bond =

Synonyms

BEK, BEP, bovine enterokinase light chain, bovine enteropeptidase, Chinese bovine enterokinase, Chinese northern yellow bovine enterokinase catalytic subunit, EC 3.4.4.8, EK, EKL, EKL-His6, EKLC, enterokinase, enterokinase light chain, enteropeptidase, enteropeptidase light chain, EP 118-1035, EP-1, EPL, HEK, HEP, human enteropeptidase, L-BEP, L-HEK, L-HEP, native enterokinase, natural enteropeptidase, peptidase, entero-, porcine enterokinase, PRSS7, recombinant bovine enterokinase catalytic subunit protein, recombinant enterokinase light chain, recombinant His-tagged enterokinase light chain, rEKL, rEKL/His, sBEKLC, TMPRSS15

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.9 enteropeptidase

Purification

Purification on EC 3.4.21.9 - enteropeptidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
amylose affinity column chromatography
anion exchange chromatography
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by gel filtration
His-Tag affinity chromatography
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HisTrap chelating column chromatography
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HiTrap Q column chromatography
native enzyme from duodenal mucosa by anion exchange and benzoamidine affinity chromatography, followed by hydrophobic interaction chromatography and gel filtration
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Ni-NTA column chromatography,
Ni2+ affinity chromatography
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of the recombinant fusion protein by osmotic shock technique and nickel affinity column chromatography
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of the recombinant fusion protein using nickel affinity column chromatography
of the recombinant fusion protein using nickel affinity column chromatography, refolding and auto-catalytic cleavage
recombinant catalytic subunit
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refolded, soluble, and detagged recombinant enteropeptidase light chain by affinity chromatography on soybean trypsin inhibitor agarose
solubilization of inclusion bodies with 6 M guanidine-HCl, affinity chromatography on STI-agarose
soybean trypsin inhibitor-agarose column chromatography, and gel filtration
STI-agarose column chromatography
STI-agarose column chromatography and Superdex 200 gel filtration
ÄKTA-purifier system and a Sepharose QFF column (HiPrep 16/10 QFF)
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