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3.4.21.89: Signal peptidase I

This is an abbreviated version!
For detailed information about Signal peptidase I, go to the full flat file.

Word Map on EC 3.4.21.89

Reaction

Cleavage of hydrophobic, N-terminal signal or leader sequences =

Synonyms

Bacterial leader peptidase 1, bacterial type I signal peptidase, big signal peptidase, canine signal peptidase complex, chloroplast processing peptidase, EC 3.4.99.36, Escherichia coli leader peptidase, Eukaryotic signal peptidase, Eukaryotic signal proteinase, hen oviduct signal peptidase, HOSP, imp1p, Leader peptidase, Leader peptidase I, Leader peptide hydrolase, Leader proteinase, LEP, LepB, PA1303, Pcp1, Peptidase, signal, Pilin leader peptidase, plastidic SPase I, plastidic SPase I 1, plastidic type I signal peptidase 1, Plsp1, Prokaryotic leader peptidase, Prokaryotic signal peptidase, Prokaryotic signal proteinase, Propeptidase, protease IV, Proteinase, eukaryotic signal, Proteinase, signal, PuIO prepilin peptidase, Rv2903c, SEC11, secretory protein 11, Signal peptidase, signal peptidase I, signal peptidase I-3, Signal peptidase IB, signal peptidase type I, Signal peptide hydrolase, Signal peptide peptidase, Signalase, Sip1, Sip2, Sip3, SipP (pTA1015), SipP (pTA1040), SipS, SipT, SipU, SipV, SipW, SipX, SipY, SipZ, SP, SP I, SP-I, SPase, Spase I, SPase I 1, SPase IB, SPaseI, SPC, Spc1, SPI, SPP, SpsB, thylakoidal processing peptidase, TPP, type I signal (leader) peptidase, type I signal peptidase, type I signal peptidase 1, type I signal peptidase Sec11a, type I signal peptidase Sec11b, type I SPase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.89 Signal peptidase I

Purification

Purification on EC 3.4.21.89 - Signal peptidase I

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by centrifugation, gel filtration and on Ni-agarose resin
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by sonication, centrifugation and gel filtration
doubly 13C, 15N- and singly 15N-isotopically labeled C-terminal SPase I DELTA2-75
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for purification purposes, tagged versions of the protein products of type I signal peptidase Sec11a and SEc11b are expressed in transformed Haloferax volcanii, with Sec11a or Sec11b being fused to a cellulose-binding domain capable of interaction with cellulose in hypersaline surroundings
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isoenzyme protease IV
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Lbpro-C51A, Lbpro-C51A-C-133S, and sLbpro-C51A-C133S
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Lep and mutant delta2-75
MBP-SipS fusion protein, SipS, SipS-P2
recombimant His-tagged wild-type and mutant SpsBs from Escherichia coli strain BL21(DE3) by ultracentrifugation and nickel affinity chromatography
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged Sip2 and Sip3 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant protein
wild-type and mutant enzymes
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