3.4.21.89: Signal peptidase I

This is an abbreviated version!
For detailed information about Signal peptidase I, go to the full flat file.

Reaction

Cleavage of hydrophobic, N-terminal signal or leader sequences =

Synonyms

Bacterial leader peptidase 1, bacterial type I signal peptidase, big signal peptidase, canine signal peptidase complex, chloroplast processing peptidase, EC 3.4.99.36, Escherichia coli leader peptidase, Eukaryotic signal peptidase, Eukaryotic signal proteinase, hen oviduct signal peptidase, HOSP, imp1p, Leader peptidase, Leader peptidase I, Leader peptide hydrolase, Leader proteinase, LEP, LepB, PA1303, Pcp1, Peptidase, signal, Pilin leader peptidase, plastidic SPase I, plastidic SPase I 1, plastidic type I signal peptidase 1, Plsp1, Prokaryotic leader peptidase, Prokaryotic signal peptidase, Prokaryotic signal proteinase, Propeptidase, protease IV, Proteinase, eukaryotic signal, Proteinase, signal, PuIO prepilin peptidase, Rv2903c, SEC11, secretory protein 11, Signal peptidase, signal peptidase I, signal peptidase I-3, Signal peptidase IB, signal peptidase type I, Signal peptide hydrolase, Signal peptide peptidase, Signalase, Sip1, Sip2, Sip3, SipP (pTA1015), SipP (pTA1040), SipS, SipT, SipU, SipV, SipW, SipX, SipY, SipZ, SP, SP I, SP-I, SPase, Spase I, SPase I 1, SPase IB, SPaseI, SPC, Spc1, SPI, SPP, SpsB, thylakoidal processing peptidase, TPP, type I signal (leader) peptidase, type I signal peptidase, type I signal peptidase 1, type I signal peptidase Sec11a, type I signal peptidase Sec11b, type I SPase

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.21 Serine endopeptidases

                3.4.21.89 Signal peptidase I

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Results	in table
15	Activating Compound
75654	AA Sequence
48	Application
1	CAS Registry Number
61	Cloned(Commentary)
7	Crystallization (Commentary)
287	Disease/ Diagnostics
104	General Information
2	General Stability
14	IC50 Value
187	Inhibitors
2	kcat/KM [mM/s]
4	Ki Value [mM]
43	KM Value [mM]
116	Localization
93	Molecular Weight [Da]
73	Natural Substrates/ Products (Substrates)
251	Organism
29	PDB ID
12	pH Optimum
5	pH Range
2	pH Stability
4	pI Value
10	Posttranslational Modification
95	Protein Variants
41	Purification (Commentary)
7	Reaction
1	Reaction Type
201	Reference
1	Renatured (Commentary)
15	Source Tissue
7	Specific Activity [micromol/min/mg]
2	Storage Stability
248	Substrates and Products (Substrate)
42	Subunits
269	Synonyms
7	Temperature Optimum [°C]
1	Temperature Range [°C]
5	Temperature Stability [°C]
57	Turnover Number [1/s]

KM Value

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KM Value on EC 3.4.21.89 - Signal peptidase I

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.05

alkaline phosphatase signal peptide fused to full-length mammalian cytochrome b5

Escherichia coli

-

pH 7.5, 37°C

650357

-

0.988

decanoyl-LT-P-TAKA-A-SKIDD-OH

Escherichia coli

-

pH 8.5, 37°C

651183

0.029

decanoyl-LTPTAKAASKIDD-OH

Staphylococcus aureus

-

pH 8.5, 37°C

651183

0.43 - 0.8

FSASALAKI

19 entries

0.33 - 0.52

Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile

2 entries

1

Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile-NH2

Escherichia coli

-

-

29806

0.0166 - 0.044

preprotein substrate PONA

4 entries

-

0.0165

pro-ompA-nuclease

Escherichia coli

-

pH 8.0

653816

-

0.0165 - 0.032

pro-OmpA-nuclease A

2 entries

-

0.319

tosyl-Gly-Pro-Lys-p-nitroanilide

Pseudomonas aeruginosa

-

pH 10.0, 25°C

651885

0.727

Val-Leu-Lys-p-nitroanilide

Pseudomonas aeruginosa

-

pH 10.0, 25°C

651885

0.0006 - 0.11

Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2

2 entries

0.0328 - 0.0354

YFSASALA-4-methylcoumarin 7-amide

5 entries

additional information

additional information

2 entries

-

0.43

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R318A

667857

0.49

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R127A

667857

0.49

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R275A

667857

0.49

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R282A

667857

0.49

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R315K

667857

0.52

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R146A

667857

0.52

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R198A

667857

0.52

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R226A

667857

0.52

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R236A

667857

0.52

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R295A

667857

0.52

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, wild-type enzyme

667857

0.53

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R222K

667857

0.53

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R318A

667857

0.54

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R222A

667857

0.55

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R199A

667857

0.57

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R315A

667857

0.63

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R77K

667857

0.67

FSASALAKI

Escherichia coli

-

37°C, pH 7.7, mutant enzyme R77A

667857

0.8

FSASALAKI

Escherichia coli

-

pH 8.1, 37°C

649998

0.33

Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile

Escherichia coli

-

-

29806

0.52

Phe-Ser-Ala-Ser-Ala-Leu-Ala-Lys-Ile

Escherichia coli

-

-

29827

0.0166

preprotein substrate PONA

Bacillus subtilis

P28628

pH 8.0, 37°C, A14PONA mutant, SipS

652064

-

0.0192

preprotein substrate PONA

Escherichia coli

P00803

pH 8.0, 37°C, Lep

652064

-

0.0425

preprotein substrate PONA

Escherichia coli

P00803

pH 8.0, 37°C, A14PONA mutant, Lep

652064

-

0.044

preprotein substrate PONA

Bacillus subtilis

P28628

pH 8.0, 37°C, SipS

652064

-

0.0165

pro-OmpA-nuclease A

Escherichia coli

-

-

29825

-

0.032

pro-OmpA-nuclease A

Escherichia coli

-

-

29826

-

0.0006

Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2

Escherichia coli

-

pH 8.1, 37°C

649998

0.11

Y-NO2-FSASALAKIK-2-aminobenzoyl-NH2

Escherichia coli

-

pH 8.1, 37°C

649998

0.0328

YFSASALA-4-methylcoumarin 7-amide

Escherichia coli

-

pH 8.1, 37°C

649998

0.0339

YFSASALA-4-methylcoumarin 7-amide

Escherichia coli

-

pH 8.1, 37°C, nD2O 0.21

649998

0.034

YFSASALA-4-methylcoumarin 7-amide

Escherichia coli

-

pH 8.1, 37°C, nD2O 0.43

649998

0.0347

YFSASALA-4-methylcoumarin 7-amide

Escherichia coli

-

pH 8.1, 37°C, nD2O 0.64

649998

0.0354

YFSASALA-4-methylcoumarin 7-amide

Escherichia coli

-

pH 8.1, 37°C, nD2O 0.85

649998

additional information

additional information

Escherichia coli

-

effect of the length of the polypeptide substrate in the Km-value

29833

-

additional information

additional information

Staphylococcus aureus

-

enzyme follows simple first-order kinetics, pseudo-first-order rate constant, overview

708475

-