Cloned (Comment) | Organism |
---|---|
cloned and overexpressed in Escherichia coli BL21(DE3)/pLysS with pKJ341 and in Escherichia coli BL21(DE3) harboring pSJS1240 | Methanococcus voltae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | archaeal enzyme cleaves the signal peptides from preproteins on the external face of the cytoplasmic membrane, thereby releasing the translocated protein from the membrane | Methanococcus voltae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21500 | - |
his-tagged version, overexpressed in Escherichia coli, Western blotting | Methanococcus voltae |
24600 | - |
deduced from DNA sequence | Methanococcus voltae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
signal peptides from preproteins + H2O | Methanococcus voltae | - |
mature proteins | - |
? | |
signal peptides from preproteins + H2O | Methanococcus voltae PS | - |
mature proteins | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanococcus voltae | - |
- |
- |
Methanococcus voltae PS | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Methanococcus voltae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Methanococcus voltae S-layer protein + H2O | truncated, his-tagged form | Methanococcus voltae | ? | - |
? | |
Methanococcus voltae S-layer protein + H2O | truncated, his-tagged form | Methanococcus voltae PS | ? | - |
? | |
signal peptides from preproteins + H2O | - |
Methanococcus voltae | mature proteins | - |
? | |
signal peptides from preproteins + H2O | - |
Methanococcus voltae PS | mature proteins | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Methanococcus voltae |