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3.4.21.89: Signal peptidase I

This is an abbreviated version!
For detailed information about Signal peptidase I, go to the full flat file.

Word Map on EC 3.4.21.89

Reaction

Cleavage of hydrophobic, N-terminal signal or leader sequences =

Synonyms

Bacterial leader peptidase 1, bacterial type I signal peptidase, big signal peptidase, canine signal peptidase complex, chloroplast processing peptidase, EC 3.4.99.36, Escherichia coli leader peptidase, Eukaryotic signal peptidase, Eukaryotic signal proteinase, hen oviduct signal peptidase, HOSP, imp1p, Leader peptidase, Leader peptidase I, Leader peptide hydrolase, Leader proteinase, LEP, LepB, PA1303, Pcp1, Peptidase, signal, Pilin leader peptidase, plastidic SPase I, plastidic SPase I 1, plastidic type I signal peptidase 1, Plsp1, Prokaryotic leader peptidase, Prokaryotic signal peptidase, Prokaryotic signal proteinase, Propeptidase, protease IV, Proteinase, eukaryotic signal, Proteinase, signal, PuIO prepilin peptidase, Rv2903c, SEC11, secretory protein 11, Signal peptidase, signal peptidase I, signal peptidase I-3, Signal peptidase IB, signal peptidase type I, Signal peptide hydrolase, Signal peptide peptidase, Signalase, Sip1, Sip2, Sip3, SipP (pTA1015), SipP (pTA1040), SipS, SipT, SipU, SipV, SipW, SipX, SipY, SipZ, SP, SP I, SP-I, SPase, Spase I, SPase I 1, SPase IB, SPaseI, SPC, Spc1, SPI, SPP, SpsB, thylakoidal processing peptidase, TPP, type I signal (leader) peptidase, type I signal peptidase, type I signal peptidase 1, type I signal peptidase Sec11a, type I signal peptidase Sec11b, type I SPase

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.89 Signal peptidase I

Cloned

Cloned on EC 3.4.21.89 - Signal peptidase I

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
19 kDa subunit encoded by SEC11 gene
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cloned and overexpressed in Escherichia coli BL21(DE3)/pLysS with pKJ341 and in Escherichia coli BL21(DE3) harboring pSJS1240
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cloning of the WT and P2 domain of SipS, MBP-SipS fusion protein expressed in Escherichia coli
doubly 13C, 15N- and singly 15N-isotopically labeled C-terminal SPase I DELTA2-75 expressed in Escherichia coli BL21(DE3) cells
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expressed in Escherichia coli BL21 (DE3) cells transfected with the pET23b-coding SPase I plasmid
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expression in Escherichia coli
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expression of a soluble form of signal peptidase, which lacks the two transmembrane domains, SPase I DELTA2-75
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expression of His-tagged wild-type and mutant SpsBs in Escherichia coli strain BL21(DE3)
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expression of mutant enzymes
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expression of wild-type and mutant enzymes in Escherichia coli
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expression vector pET16b-spi directs synthesis of full length SPase I, transformed into Escherichia coli BL21(DE3)pLysS and overexpressed
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for purification purposes, tagged versions of the protein products of type I signal peptidase Sec11a and SEc11b are expressed in transformed Haloferax volcanii, with Sec11a or Sec11b being fused to a cellulose-binding domain capable of interaction with cellulose in hypersaline surroundings
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gene lepB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene sipM, library construction, DNA and amino acid sequence determination and analysis, bicistronic operon, genetic organization and transcription analysis, overview, functional heterologous overexpression by Bacillus megaterium protoplast transformation with glucanase used as an extracellular reporter
gene SppA similar to Escherichia coli signal peptide peptidase A
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genes sip2 and sip3, expression of His-tagged isozymes Sip2 and Sip3 in Escherichia coli strain BL21(DE3)
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isoenzyme protease IV overexpressed in Escherichia coli, gene sppA
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lepB gene cloned and sequenced
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lepB gene PCR amplified
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methods for the identification of the leader peptidase gene and genetic manipulations to determine the role of leader peptidase in cell growth
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overexpression in Escherichia coli
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plasmid pMC-Spase encoding signal peptidase transformed and amplified in Escherichia coli TB1
Sec11 gene product identified as 18 kDa subunit, cloning of the gene encoding imp1p
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sip(ba) gene cloned and expressed in Escherichia coli, complementation of LepBts mutant
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SPase I isozymes, expression of His-tagged Sip2 and Sip3 in Escherichia coli strain BL21(DE3), subcloning in strain TG1
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the type I signal peptidase is an intron-less and single-copy gene