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Literature summary for 3.4.21.89 extracted from
- Catalytic efficiency of signal peptidase I of Escherichia coli is comparable to that of members of the serine protease family (1997), Protein Eng., 10, 1057-1060.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | associated with the cytoplasmic membrane | Escherichia coli | 5737 | - |
| plasma membrane | associated with the cytoplasmic membrane | Escherichia coli | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
1 * 23000 + 1 * 19000, gp23 and p19 | Escherichia coli |
| 23000 | - |
1 * 23000 + 1 * 19000, gp23 and p19 | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| signal peptides from preproteins + H2O | Escherichia coli | - |
mature proteins | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | secretory proteins are synthesized with an aminoterminal extension, the signal peptide, signal peptidases are required for the removal of these extensions | Escherichia coli | ? | - |
? | |
| pro-ompA-nuclease + H2O | hybrid secretory precursor, best substrate in vitro, fusion protein consisting of the signal peptide of the E. coli outer membrane protein A OmpA attached to the Staphylococcus aureus nuclease A protein | Escherichia coli | ompA-nuclease + ? | - |
? | |
| signal peptides from preproteins + H2O | - |
Escherichia coli | mature proteins | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 23000 + 1 * 19000, gp23 and p19 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Spase I | - |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | signal peptidase I | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
after 40°c there is a marked decrease in the rate that is due to the denaturationof Spase I | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 1.97 | pro-ompA-nuclease | pH 8.0, 37°C | Escherichia coli | |
| 5 | - |
pro-ompA-nuclease | pH 8.0, 37.5°C | Escherichia coli | |
| 8.73 | - |
pro-ompA-nuclease | pH 8.0, 37°C | Escherichia coli |
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Release 2023.1 (January 2023)
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