3.4.19.12: ubiquitinyl hydrolase 1

This is an abbreviated version, for detailed information about ubiquitinyl hydrolase 1, go to the full flat file.

Reaction

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) =

Synonyms

A20, AD-019, AMSH, associated molecule with the SH3-domain of STAM, AT-3, ataxin-3, BAP1, BPLF1, BRCA1-associated protein-1, C-terminal hydrolases UCHL3, C-terminal ubiquitin hydrolase, Cezanne, CGI-70, CYLD, de-ubiquitinating enzyme, deubiquinating isopeptidase T, deubiquitinating enzyme, Doa4, Doa4 ubiquitin hydrolase, Doa4p ubiquitin isopeptidase, DUB, dUCH, esterase, ubiquitin thiol-, hydrolase, ubiquitin carboxyl-terminal, hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch), Iso-T, isopeptidase, isopeptidase T, More, Neuron cytoplasmic protein 9.5, neuronal-specific protein gene product 9.5, OTU-1, ovarian tumour 1, PA-700 associated isopeptidase, papain-like protease, PGP 9.5, PGP 9.5/UCHL1, PGP9.5, PGP9.5.1, PLpro, Ub isopeptidase, ubiquitin C terminal hydrolase 1, ubiquitin C-terminal hydrolase, ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch), ubiquitin C-terminal hydrolase 1, ubiquitin C-terminal hydrolase 37, ubiquitin C-terminal hydrolase 8, ubiquitin C-terminal hydrolase isoform L3, ubiquitin C-terminal hydrolase L-1, ubiquitin C-terminal hydrolase L1, ubiquitin C-terminal hydrolase L3, Ubiquitin C-terminal hydrolase UCH37, ubiquitin C-terminal hydrolase-1, ubiquitin C-terminal hydrolase-L1, ubiquitin C-terminal hydrolase-L3, ubiquitin C-terminal hydrolase-L5, ubiquitin C-terminal hydrorase-L1, ubiquitin carboxy terminal hydrolase-L1, ubiquitin carboxy terminal hydrolase-L3, ubiquitin carboxy-terminal hydrolase, ubiquitin carboxy-terminal hydrolase 1, ubiquitin carboxy-terminal hydrolase L1, ubiquitin carboxy-terminal hydrolase-L1, ubiquitin carboxy-terminal hydrolase-L3, ubiquitin carboxyhydrolase L3, ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase 1, ubiquitin carboxyl terminal hydrolase L1, ubiquitin carboxyl-terminal hydrolase, ubiquitin carboxyl-terminal hydrolase 1, ubiquitin carboxyl-terminal hydrolase 44, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal hydrolase L-1, ubiquitin carboxyl-terminal hydrolase L1, ubiquitin carboxyl-terminal hydrolase l3, ubiquitin carboxyl-terminal hydrolase L5, ubiquitin carboxyl-terminal hydrolase-L1, ubiquitin carboxyterminal hydrolase L1, ubiquitin carboxyterminal hydrolase L3, ubiquitin carboxyterminal hydrolase-L1, ubiquitin hydrolase, ubiquitin hydrolase Uch-L1, ubiquitin hydrolase UCH-L3, ubiquitin isopeptidase, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, Ubiquitin thiolesterase L3, Ubiquitin thiolesterase L4, Ubiquitin thiolesterase L5, ubiquitin-C-terminal hydrolase L1, ubiquitin-carboxyl-terminal hydrolase PGP-9.5, ubiquitin-specific protease 44, UBPY, UCH, UCH L-1, UCH L1, UCH-1, UCH-8, UCH-L1, UCH-L2, UCH-L3, UCH-L4, UCH-L5, UCH37, UCH54, UCHL-1, UCHL-3, UCHL1, UCHL1/PGP 9.5, UCHL2, Uchl3, UCHL5, UCHL5N240, USP19, USP22, USP44, yeast ubiquitin hydrolase, YUH, YUH1

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.12 ubiquitinyl hydrolase 1

Substrates Products

Substrates Products on EC 3.4.19.12 - ubiquitinyl hydrolase 1

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(His)8-Ub-CTAPIII + H2O
(His)8-Ub + CTAPII
show the reaction diagram
-
Ub i.e ubiquitin, CTAPII i.e. connective tissue-activating peptide
-
?
Ac-ALRGG-7-amido-4-trifluoromethylcoumarin
?
show the reaction diagram
Ac-LRGG-7-amido-4-trifluoromethylcoumarin
?
show the reaction diagram
Ac-RLRGG-7-amido-4-trifluoromethylcoumarin
?
show the reaction diagram
activity-based haemagglutinin-tagged ubiquitin + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-Tubulin + H2O
?
show the reaction diagram
-
-
-
-
?
beta-tubulin + H2O
?
show the reaction diagram
-
-
-
-
?
branched di-ubiquitin + H2O
ubiquitin
show the reaction diagram
branched polyubiquitin + H2O
ubiquitin
show the reaction diagram
conjugate of histone H2A and ubiquitin + H2O
ubiquitin + histone H2A
show the reaction diagram
di-ubiquitin + H2O
ubiquitin
show the reaction diagram
EBV ribonucleotide reductase
?
show the reaction diagram
is deubiquitinated by BPLF1 1-246
-
-
?
HCF-1 + H2O
?
show the reaction diagram
HCF-1 is the principal Bap1-interacting protein. It is K48- and K63-ubiquitinated. Endogenous HCF-1C is ubiquitinated at lysine-1807 and/or lysine-1808
-
-
?
head-to-tail polyubiquitin + H2O
ubiquitin
show the reaction diagram
-
recombinant substrate from expression in Escherichia coli
-
-
?
hemagglutinin-tagged ubiquitin-vinyl methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
inhibitor of kappa B-alpha + H2O
?
show the reaction diagram
-
UCHL1 deubiquitinates, thereby resulting in nuclear factor-kappa B inactivation
-
-
?
K48-linked tetraubiquitin + H2O
K48-linked diubiquitin + diubiquitin
show the reaction diagram
-
-
-
-
?
K63-linked polyubiquitin + H2O
K63-linked polyubiquitin + ubiquitin
show the reaction diagram
-
-
-
-
?
linear ubiquitin-beta-galactosidase + H2O
ubiquitin + protein
show the reaction diagram
monoubiquitin + H2O
?
show the reaction diagram
N2-L-Asp-ubiquitin + H2O
ubiquitin + L-Asp
show the reaction diagram
-
-
-
?
N2-Lys-ubiquitin + H2O
ubiquitin + Lys
show the reaction diagram
-
ubiquitin amide of the alpha amino group of lysine
-
?
N6-(N2-acetyl)Lys-ubiquitin + H2O
ubiquitin + N2-acetyl-Lys
show the reaction diagram
-
ubiquitin amide of the alpha amino group of lysine
-
?
N6-Lys-ubiquitin + H2O
ubiquitin + Lys
show the reaction diagram
Nalpha-(diubiquitin)-[L-Lys] + H2O
?
show the reaction diagram
-
K48 linked diubiquitin
-
-
?
Nalpha-ubiquitin-[MQIFVRPR] + H2O
ubiquitin + MQIFVRPR
show the reaction diagram
-
-
-
-
?
Nedd-8 + H2O
?
show the reaction diagram
NEDD8-protein + H2O
NEDD8 + protein
show the reaction diagram
enzyme shows dual specificity for ubiquitin-conjugates and NEDD8-conjugates
-
?
Nepsilon-(diubiquitin)-[Nalpha-actyl-L-Lys] + H2O
?
show the reaction diagram
-
K48 linked diubiquitin
-
-
?
poly-His tagged di-ubiquitin + H2O
ubiquitin + ?
show the reaction diagram
-
-
-
-
?
polyubiquitin + H2O
ubiquitin
show the reaction diagram
Smad2 + H2O
?
show the reaction diagram
-
weaker association
-
-
?
Smad3 + H2O
?
show the reaction diagram
-
weaker association
-
-
?
Smad7 + H2O
?
show the reaction diagram
-
association with Smad7, which can act as an adaptor able to recruit UCH37 to the type I TGF-beta receptor and reverses Smurf-mediated ubiquitination
-
-
?
spermidine-ubiquitin + H2O
ubiquitine + spermidine
show the reaction diagram
-
-
-
-
?
type I TGF-beta receptor + H2O
?
show the reaction diagram
ubiquitin + H2O
?
show the reaction diagram
ubiquitin + H2O
monoubiquitin
show the reaction diagram
-
UCH-L1 removes and recycles ubiquitin molecules from degraded proteins
-
-
?
ubiquitin 7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin C-terminal amide + H2O
ubiquitin + NH3
show the reaction diagram
-
-
-
-
?
ubiquitin C-terminal-4-methyl-7-amido-coumarin + H2O
ubiquitin + 4-methyl-7-amino-coumarin
show the reaction diagram
-
UCHL3 is 200fold more active than UCHL1/PGP 9.5
-
-
?
ubiquitin ethyl ester + H2O
ubiquitin + ethanol
show the reaction diagram
ubiquitin ethylester + H2O
ubiquitin + an alcohol
show the reaction diagram
-
synthetic substrate
-
-
?
ubiquitin fused to 60% of its N-terminus + H2O
ubiquitin + 60% of ubiquitin N-terminus
show the reaction diagram
-
recombinant substrate, expressed in Escherichia coli
-
?
ubiquitin glycine methyl ester + H2O
ubiquitin + methanol
show the reaction diagram
-
-
-
-
?
ubiquitin metallothionein + H2O
?
show the reaction diagram
ubiquitin thiol ester of dithiothreitol + H2O
ubiquitin + dithiothreitol
show the reaction diagram
-
-
-
-
?
ubiquitin-4-methylcoumarin 7-amide + H2O
ubiquitin + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
ubiquitin-4-methylcoumarine 7-amide + H2O
ubiquitin + 7-amino-4-methylcoumarine
show the reaction diagram
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
show the reaction diagram
ubiquitin-alcohol + H2O
ubiquitin + alcohol
show the reaction diagram
-
-
-
ir
ubiquitin-alpha-NH-dihydrofolate reductase + H2O
ubiquitin + dihydrofolate reductase
show the reaction diagram
-
-
-
-
?
ubiquitin-alpha-NH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
show the reaction diagram
-
-
-
?
ubiquitin-alphaNH-MHISPPEPESEEEEHYC + H2O
ubiquitin + MHISPPEPESEEEEHYC
show the reaction diagram
ubiquitin-AMC
?
show the reaction diagram
BPLF1 1-246 expresses deubiquitinating activity, cleaves ubiquitin chains linked by K48 and K63
-
-
?
ubiquitin-AMC + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-AW + H2O
?
show the reaction diagram
ubiquitin-B domain of staphylococcal protein-A + H2O
ubiquitin + B domain of staphylococcal protein-A
show the reaction diagram
-
and variants thereof, substrate is expressed as alpha-linked C-terminal fusion to ubiquitin
-
-
?
ubiquitin-beta1 domain of streptococcal protein G + H2O
ubiquitin + beta1 domain of streptococcal protein G
show the reaction diagram
-
and variants thereof, substrate is expressed as alpha-linked C-terminal fusion to ubiquitin
-
-
?
ubiquitin-carboxyl extension protein + H2O
ubiquitin + ubiquitin-carboxyl extension protein
show the reaction diagram
ubiquitin-CEP52 + H2O
ubiquitin + CEP52
show the reaction diagram
ubiquitin-CEP80 + H2O
ubiquitin + CEP80
show the reaction diagram
ubiquitin-Cys + H2O
ubiquitin + Cys
show the reaction diagram
-
-
-
?
ubiquitin-Cys-Cys + H2O
ubiquitin + Cys-Cys
show the reaction diagram
-
-
-
?
ubiquitin-Fur4p-conjugate + H2O
ubiquitin + Fur4p
show the reaction diagram
-
ubiquitin is bound to the Fur4p protein via its Lys63
-
?
ubiquitin-human carboxyl extension protein fusion protein + H2O
ubiquitin + human carboxyl extension protein
show the reaction diagram
ubiquitin-magainin + H2O
magainin + ubiquitin
show the reaction diagram
-
is efficiently cleaved at the carboxyl terminus of ubquitin, yielding recombinant magainin with high antimicrobial activity
-
-
?
ubiquitin-peptide + H2O
?
show the reaction diagram
ubiquitin-piscidin 1 + H2O
ubiquitin + piscidin 1
show the reaction diagram
-
-
-
?
ubiquitin-protein + H2O
ubiquitin + protein
show the reaction diagram
ubiquitin-rhodamine110-glycine + H2O
rhodamine110
show the reaction diagram
-
-
-
-
?
ubiquitin-W + H2O
?
show the reaction diagram
ubiquitin-W-G75A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-H68A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-I44A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-K11A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-K48A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-K63A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-K6A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-L71A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-L73A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-R42A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-R72A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-R74A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-W-V70A + H2O
?
show the reaction diagram
-
-
-
-
?
ubiquitin-WA + H2O
?
show the reaction diagram
ubiquitinyl-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
show the reaction diagram
ubiquitinyl-peptide + H2O
ubiquitin + peptide
show the reaction diagram
ubiquitinyldihydrofolate reductase + H2O
ubiquitin + dihydrofolate reductase
show the reaction diagram
-
UCH-1
-
?
Z-Arg-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-