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Literature summary for 3.4.19.12 extracted from
- AMSH is an endosome-associated ubiquitin isopeptidase (2004), J. Cell Biol., 166, 487-492.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as GST-tagged enzyme | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D348A | site-directed mutagenesis, inactive mutant, leads to accumulation of ubiquitin on endosomes and the concomitant stabilization of an ubiquinated form of the signal transducing adeptor molecule, STAM | Homo sapiens |
| additional information | enzyme downregulation in HeLa cells by siRNA, enzyme inhibition influences the degradation of the EGF receptor | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is inhibited by zinc-chelating agents | Homo sapiens | |
| NEM | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endosome | associated to | Homo sapiens | 5768 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | AMSH contains the JAMM motif, i.e. JAB1/MPN/Mov34 metalloenzyme motif, in the active site | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | AMSH is a deubiquinating enzyme with functions at the endosome, it opposes the ubiquitin-dependent sorting of receptors to lysosomes, the enzyme influences the degradation of the EGF receptor by association to endosomes, model of the enzyme's physiological function | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| K48-linked tetraubiquitin + H2O | - |
Homo sapiens | K48-linked diubiquitin + diubiquitin | - |
? | |
| K63-linked polyubiquitin + H2O | - |
Homo sapiens | K63-linked polyubiquitin + ubiquitin | - |
? | |
| additional information | AMSH is a deubiquinating enzyme with functions at the endosome, it opposes the ubiquitin-dependent sorting of receptors to lysosomes, the enzyme influences the degradation of the EGF receptor by association to endosomes, model of the enzyme's physiological function | Homo sapiens | ? | - |
? | |
| additional information | AMSH interacts with the signal transducing adaptor molecule, STAM, via the SH3-domains | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | AMSH contains the JAMM motif, i.e. JAB1/MPN/Mov34 metalloenzyme motif, in the active site | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMSH | - |
Homo sapiens |
| associated molecule with the SH3-domain of STAM | - |
Homo sapiens |
| ubiquitin isopeptidase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8.3 | broad optimum | Homo sapiens |
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Release 2023.1 (January 2023)
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