Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.12 extracted from

  • Yu, H.A.; Kim, S.G.; Kim, E.J.; Lee, W.J.; Kim, D.O.; Park, K.; Park, Y.C.; Seo, J.H.
    Characterization of ubiquitin C-terminal hydrolase 1 (YUH1) from Saccharomyces cerevisiae expressed in recombinant Escherichia coli (2007), Protein Expr. Purif., 56, 20-26.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
two plasmids with two types of YUH1 with the 6 histidine tag at the N-terminus (H6YUH1) or C-terminus (YUH1H6) expressed in Escherichia coli DH5alpha Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Ni-NTA affinity chromatography, to more than 90% homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.61
-
YUH1H6 Saccharomyces cerevisiae
1.72
-
H6YUH1 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information H6YUH1 is unable to hydrolyze the linkage of ubiquitin with human growth hormone Saccharomyces cerevisiae ?
-
?
Z-Arg-Leu-Arg-Gly-Gly-7-amido-4-methylcoumarin + H2O
-
Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
ubiquitin C-terminal hydrolase 1
-
Saccharomyces cerevisiae
YUH1
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
-
Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
H6YUH1 shows 3.2 and 1.4 h of half lives at 4°C and 52°C, respectively. Immobilized YUH1 in 50% glycerol solution at -20°C results in 52% of decrease in specific activity for 7 days, corresponding to a 2.7fold increase compared with that of free YUH1 incubated in the same solution at 4°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Saccharomyces cerevisiae