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3.2.1.62: glycosylceramidase

This is an abbreviated version!
For detailed information about glycosylceramidase, go to the full flat file.

Word Map on EC 3.2.1.62

Reaction

a beta-D-galactosyl-N-acylsphingosine
+
H2O
=
a ceramide
+
beta-D-galactose

Synonyms

cerebrosidase, EGC, EGCII, EGCrP1, Egh1, endo-glycoceramidase II, endoglycoceramidase II, endoglycoceramidase-related protein 1, ergosteryl-beta-glucosidase, Gcase, glucocerebrosidase, glycosyl ceramide glycosylhydrolase, glycosyl-N-acylsphingosine glycohydrolase, glycosylceramidase, KLotho lactase phlorizin hydrolase, Klotho-related protein, lactase phlorizin hydrolase, lactase-phlorizin hydrolase, LCT, LPH, LPH1, phloretin-glucosidase, phloridzin glucosidase, phlorizin beta-glucosidase, phlorizin hydrolase, Yir007w

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.62 glycosylceramidase

Crystallization

Crystallization on EC 3.2.1.62 - glycosylceramidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the recombinant protein with the hanging drops by vapor diffusion method
-
by vapor diffusion in the presence of detergents like Triton X-100, octyl beta-D-glucopyranoside, decyl beta-D-maltopyranoside, and dodecyl beta-D-maltopyranoside, form, in complex with the ganglioside GM3
-
hanging drop vapor diffusion method, using 1% (v/v) Triton X-100, 10% (v/v) glycerol, and 25% (w/v) PEG